A5009281496- Probiotics and Fermented Foods
- Gut microbiota and health
- Microfluidic and Capillary Electrophoresis Applications
- Microbial Metabolites in Food Biotechnology
- Cell death mechanisms and regulation
- Digestive system and related health
- Infant Nutrition and Health
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Food composition and properties
- Cardiac, Anesthesia and Surgical Outcomes
- Immune Cell Function and Interaction
- Global Health Workforce Issues
- Dental Education, Practice, Research
- Phagocytosis and Immune Regulation
- Immunotherapy and Immune Responses
- Electrowetting and Microfluidic Technologies
- Inflammatory Bowel Disease
- Hepatitis C virus research
- Thyroid Disorders and Treatments
- Education Systems and Policy
- Chemical Synthesis and Analysis
- Mycobacterium research and diagnosis
- Infant Health and Development
- Analytical Chemistry and Sensors
University of California, San Diego
2021
University College London
2021
University of Würzburg
2012-2016
Walter de Gruyter (Germany)
2014-2016
Friedrich-Alexander-Universität Erlangen-Nürnberg
2014-2016
Mercy University Hospital
1992-2014
Notre Dame of Dadiangas University
2014
University of California, Irvine
2003-2013
Center for Human Reproduction
2013
Biopico Systems (United States)
2013
Tumors escape immunological rejection by a diversity of mechanisms. In this report, we demonstrate that the colon cancer cell SW620 expresses functional Fas ligand (FasL), triggering agent receptor (FasR)-mediated apoptosis within immune system. FasL mRNA and surface were detected in cells using reverse transcription polymerase chain reaction (RT-PCR) immunohistochemical staining, respectively. We show kills Jurkat T Fas-mediated manner. FasR-specific antisense oligonucleotide treatment,...
ABSTRACT Spray drying of skim milk was evaluated as a means preserving Lactobacillus paracasei NFBC 338 and salivarius UCC 118, which are human-derived strains with probiotic potential. Our initial experiments revealed that is considerably more heat resistant in 20% (wt/vol) than 118 is; the comparable decimal reduction times were 11.1 1.1 min, respectively, at 59°C. An air outlet temperature 80 to 85°C optimal for spray drying; these conditions resulted powders moisture contents 4.1 4.2%...
Various cancer cell lines express Fas ligand (FasL) and can kill lymphoid cells by Fas-mediated apoptosis in vitro. FasL expression has been demonstrated several human malignancies vivo. We sought to determine whether esophageal carcinomas FasL, is associated with increased of tumor-infiltrating lymphocytes (TIL) vivo, thereby contributing the immune privilege tumor. Using situ hybridization immunohistochemistry, respectively, mRNA protein were colocalized neoplastic epithelial all...
Background: The enteric bacterial flora has been implicated in the pathogenesis of enterocolitis and colon cancer C57BL/6 IL‐10 knockout mice. Probiotic Lactobacilli modify are thought to have a beneficial effect on enterocolitis. We conducted controlled feeding trial mice using probiotic Lactobacillus salivarius ssp. s alivarius UCC118. Aim: To determine consumption gastrointestinal microflora, tumour development colitis Methods: Twenty were studied (10 consumed organisms milk 10 unmodified...
ABP-118, a small heat-stable bacteriocin produced by Lactobacillus salivarius subsp. UCC118, strain isolated from the ileal–caecal region of human gastrointestinal tract, was purified to homogeneity. Using reverse genetics, DNA fragment specifying part ABP-118 identified on 10769 bp chromosomal region. Analysis this revealed that Class IIb two-peptide composed Abp118α, which exhibited antimicrobial activity, and Abp118β, enhanced activity. The gene conferring UCC118 immunity action abpIM,...
ABSTRACT Cheddar cheese was manufactured with either Lactobacillus salivarius NFBC 310, 321, or 348 L. paracasei 338 364 as the dairy starter adjunct. These five strains had previously been isolated from human small intestine and have characterized extensively respect to their probiotic potential. Enumeration of these in mature cheese, however, complicated by presence high numbers (>10 7 CFU/g cheese) nonstarter lactic acid bacteria, principally composed lactobacilli which proliferate...
Summary A role for the intestinal microbiota is routinely cited as a potential aetiological factor in colorectal cancer initiation and progression. As majority of bacteria gut are refractory to culture we investigated this ecosystem subjects with adenomatous polyposis who at high risk developing cancer, using culture‐independent methods. Twenty 20 polypectomized volunteers were chosen analysis. An exploration diversity temporal stability dominant several bacterial subgroups was undertaken...
Lactobacillus salivarius subsp. strain UCC118 is a bacteriocin-producing with probiotic characteristics. The 2.13-Mb genome was shown by sequencing to comprise 1.83 Mb chromosome, 242-kb megaplasmid (pMP118), and two smaller plasmids. Megaplasmids previously have not been characterized in lactic acid bacteria or intestinal lactobacilli. Annotation of the sequence indicated an intermediate level auxotrophy compared other sequenced No single-copy essential genes were located on megaplasmid....
ABSTRACT The viability of the human probiotic strains Lactobacillus paracasei NFBC 338 and Bifidobacterium sp. strain UCC 35612 in reconstituted skim milk was assessed by confocal scanning laser microscopy using LIVE/DEAD Bac Light stain. technique rapid (<30 min) clearly differentiated live from heat-killed bacteria. microscopic enumeration various proportions viable to bacteria then compared with conventional plating on nutrient agar. Direct indicated that plate counting led an...
Continuous flow microreactors with an annular microchannel for cyclical chemical reactions were fabricated by either bulk micromachining in silicon or rapid prototyping using EPON SU-8. Fluid propulsion these unusual microchannels was achieved AC magnetohydrodynamic (MHD) actuation. This integrated micropumping mechanism obviates the use of moving parts acting locally on electrolyte, exploiting its inherent conductive nature. Both and SU-8 capable MHD actuation, attaining fluid velocities...
We previously reported the use of laser capture microdissection (LCM) and PCR to detect presence Mycobacterium paratuberculosis DNA in granulomas patients with Crohn's disease. While this does not imply a cause-effect relationship, it may influence disease process because bacterial has immunomodulatory effects. The aim study was determine whether from nonmycobacterial commensals, such as Escherichia coli, is also increased disease.Archival tissue 15 surgical cases 10 non-Crohn's...