A5068716378- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- Antibiotics Pharmacokinetics and Efficacy
- Protein Degradation and Inhibitors
- RNA Research and Splicing
- Parathyroid Disorders and Treatments
- Lipid Membrane Structure and Behavior
- Immune Cell Function and Interaction
- Analytical Chemistry and Chromatography
- NF-κB Signaling Pathways
- Iron Metabolism and Disorders
- Genomics and Chromatin Dynamics
- Child Nutrition and Water Access
- Chromatin Remodeling and Cancer
- Analytical Methods in Pharmaceuticals
- Peptidase Inhibition and Analysis
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Genetic and Kidney Cyst Diseases
- Cytokine Signaling Pathways and Interactions
- Genetics and Neurodevelopmental Disorders
Medical University of Vienna
2006-2021
Austrian Competence Centre of Food Safety
2021
University of Applied Sciences Technikum Wien
2021
Max Perutz Labs
2007-2010
Vienna Biocenter
2009-2010
Weizmann Institute of Science
2001-2004
Histone deacetylases (HDACs) catalyze the removal of acetyl groups from core histones.Because their capacity to induce local condensation chromatin, HDACs are generally considered repressors transcription.In this report, we analyzed role class I histone deacetylase HDAC1 as a transcriptional regulator by comparing expression profiles wild-type and HDAC1-deficient embryonic stem cells.A specific subset mouse genes (7%) was deregulated in absence HDAC1.We identified several putative tumor...
Histone deacetylases (HDACs) are chromatin-modifying enzymes that involved in the regulation of proliferation, differentiation and development. HDAC inhibitors induce cell cycle arrest, differentiation, or apoptosis tumor cells therefore promising antitumor agents. Numerous genes were found to be deregulated upon inhibitor treatment; however, relevant target still unidentified. HDAC1 is required for mouse development unrestricted proliferation embryonic stem cells. We show here reversibly...
Chromatin modifications, such as reversible histone acetylation, play a key role in the regulation of T cell development and function. However, individual deacetylases (HDACs) cells is less well understood. In this article, we show by conditional gene targeting that cell-specific loss HDAC1 led to an increased inflammatory response vivo allergic airway inflammation model. Mice with HDAC1-deficient displayed increase all critical parameters Th2-type asthma model, eosinophil recruitment into...
A subgroup of genes induced by IFN-γ requires both STAT1 and IRF1 for transcriptional activation. Using WT, stat1 −/− , or irf1 cells, we analyzed the changes in gbp2 promoter chromatin. associated with independently played an essential role ordered recruitment coactivator/histone acetyl transferase CREB-binding protein (CBP) histone deacetylase HDAC1. Hyperacetylation 4 also required STAT1. Phosphorylation at S727 transactivating domain increased activity In cells expressing a...
Histone deacetylases (HDACs) are negative regulators of gene expression and have been implicated in tumorigenesis tumor progression. Therefore, HDACs promising targets for anti-tumor drugs. However, the relevant isoforms 18 members encompassing HDAC family not identified. Studies utilizing either targeting or knockdown approaches reveal both specific redundant functions closely related class I HDAC1 HDAC2 control proliferation differentiation. Combined ablation different cell types led to a...
Histone deacetylase inhibitors induce cell cycle arrest and apoptosis in tumor cells are, therefore, promising anti-cancer drugs. The cyclin-dependent kinase inhibitor p21 is activated histone (HDAC) inhibitor-treated cells, its growth-inhibitory function contributes to the anti-tumorigenic effect of HDAC inhibitors. We show here that induction by trichostatin A involves MAP signaling. Activation signaling pathway growth factors or stress signals results H3 serine 10 phosphorylation at...
Erosion of the epigenetic DNA methylation landscape is a widely recognized hallmark aging. Emerging advances in high throughput sequencing techniques, particular data analysis, have resulted establishment precise human and murine age prediction tools. In vertebrates, cytosine at C5 position CpG dinucleotides executed by methyltransferases (DNMTs) whereas process enzymatic demethylation highly dependent on activity ten-eleven translocation methylcytosine dioxygenase (TET) family enzymes....
Aging is associated with profound changes in the epigenome, resulting alterations of gene expression, epigenetic landscape, and genome architecture. Class I Histone deacetylases (HDACs), consisting HDAC1, HDAC2, HDAC3, HDAC8, play a major role regulation chromatin structure transcriptional control, have been implicated as key players pathogenesis age-dependent diseases disorders affecting health longevity. Here, we report identification class Hdac orthologs their detailed spatio-temporal...
The founding member of the sirtuin family, yeast Sir2, was first evolutionarily conserved gene to be identified as a regulator longevity. Sirtuins constitute protein family metabolic sensors, translating changes in NAD + levels into adaptive responses, thereby acting crucial regulators network that controls energy homeostasis and such determines healthspan. In mammals comprises seven proteins, SIRT1-SIRT7, which vary tissue specificity, subcellular localization, enzymatic activity targets....
Prolonging the circulatory half-life of low mass protein drugs can be achieved either by administration a pro-drug or through co-injection with carrier that will slowly release active protein. The rate is concentration and affinity dependent. optimal relationship between these two in prolonging focus this work. Interferon (IFN) β one most widely used clinic. Here, we show IFNβ significantly extended co-administration extracellular domain IFN receptor ifnar2 (ifnar2-EC). To investigate...
Members of the Klotho gene family have been identified as modulators aging process. Deletion αklotho in mouse results a syndrome resembling rapid human aging. Conversely, overexpression extends mammalian lifespan. Here, we identify klotho orthologs vertebrate model Nothobranchius furzeri and provide detailed spatio-temporal expression profile both paralogs, α βklotho, from embryogenesis until old age spanning entire life cycle organism. Specifically, observe low levels paralogs during...
Actively transcribed regions of the genome have been found enriched for histone H3 variant H3.3. This is incorporated into nucleosomes throughout cell cycle whereas canonical isoforms are predominately deposited in association with replication. In order to obtain a global picture deposition pattern at single level we expressed H3.3 both normal and malignant human cells analyzed nuclei using conventional structured illumination imaging (SIM). We that distribution interphase differs from...