A5005308199- Neurobiology and Insect Physiology Research
- Invertebrate Immune Response Mechanisms
- Animal Behavior and Reproduction
- Genetics, Aging, and Longevity in Model Organisms
- Insect Resistance and Genetics
- Insect Utilization and Effects
- Insect and Arachnid Ecology and Behavior
- Plant Molecular Biology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Genetics and Reproduction
- Mitochondrial Function and Pathology
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- Developmental Biology and Gene Regulation
- Adipose Tissue and Metabolism
- Physiological and biochemical adaptations
- Epigenetics and DNA Methylation
- Estrogen and related hormone effects
- Lipid metabolism and biosynthesis
- Circadian rhythm and melatonin
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Retinoids in leukemia and cellular processes
- Antioxidant Activity and Oxidative Stress
- Nuclear Receptors and Signaling
- Silk-based biomaterials and applications
University of Utah
2015-2024
Howard Hughes Medical Institute
1997-2022
University of California, Los Angeles
2016
National Institutes of Health
2016
Biomedical Informatics Research Center Antwerp
1993-2011
Salk Institute for Biological Studies
2002
Stanford University
1986-1990
University of California, Berkeley
1981-1983
Pyruvate constitutes a critical branch point in cellular carbon metabolism. We have identified two proteins, Mpc1 and Mpc2, as essential for mitochondrial pyruvate transport yeast, Drosophila, humans. Mpc2 associate to form an ~150-kilodalton complex the inner membrane. Yeast Drosophila mutants lacking MPC1 display impaired metabolism, with accumulation of upstream metabolites depletion tricarboxylic acid cycle intermediates. Loss yeast results defective uptake, silencing or MPC2 mammalian...
ABSTRACT During insect metamorphosis, pulses of the steroid hormone 20-hydroxyecdysone (ecdysone) direct destruction obsolete larval tissues and their replacement by structures that form adult fly. We show here midgut salivary gland histolysis are stage-specific steroid-triggered programmed cell death responses. Dying nuclei become permeable to vital dye acridine orange DNA undergoes fragmentation, indicative apoptosis. Furthermore, these can be inhibited ectopic expression baculovirus...
Living organisms, from bacteria to humans, display a coordinated transcriptional response xenobiotic exposure, inducing enzymes and transporters that facilitate detoxification. Several transcription factors have been identified in vertebrates contribute this regulatory response. In contrast, little is known about pathway insects. Here we show the Drosophila Nrf2 (NF-E2-related factor 2) ortholog CncC (cap ‘n’ collar isoform-C) central regulator of detoxification responses. A binding site for...
Abstract Background The steroid hormone 20-hydroxyecdysone (20E) triggers the major developmental transitions in Drosophila , including molting and metamorphosis, provides a model system for defining molecular mechanisms of signaling. 20E acts via heterodimer two nuclear receptors, ecdysone receptor (EcR) Ultraspiracle, to directly regulate target gene transcription. Results Here we identify genomic transcriptional response as well those genes that are dependent on EcR their proper...
ABSTRACT During Drosophila third instar larval development, one or more pulses of the steroid hormone ecdysone activate three temporally distinct sets genes in salivary glands, represented by puffs polytene chromosomes. The intermolt are induced first, midthird larvae; these encode a protein glue used animal to adhere itself solid substrate for metamorphosis. repressed at puparium formation as high titer pulse directly induces small set early regulatory genes. both repress their own...