A5074612463- Cytomegalovirus and herpesvirus research
- Sarcoma Diagnosis and Treatment
- Herpesvirus Infections and Treatments
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Toxoplasma gondii Research Studies
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Cardiac tumors and thrombi
- Circular RNAs in diseases
- Cancer Genomics and Diagnostics
- interferon and immune responses
- Lymphoma Diagnosis and Treatment
- Vascular Tumors and Angiosarcomas
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- Autophagy in Disease and Therapy
- Angiogenesis and VEGF in Cancer
- Environmental remediation with nanomaterials
- CAR-T cell therapy research
- Advanced oxidation water treatment
- Clostridium difficile and Clostridium perfringens research
- Cancer, Hypoxia, and Metabolism
- Epilepsy research and treatment
University of Macau
2025
China Pharmaceutical University
2025
Westlake University
2022-2024
China Medical University
2013-2024
Shanghai Sixth People's Hospital
2014-2024
Guangdong University of Technology
2024
Shanghai University
2024
Taizhou People's Hospital
2017-2024
Nantong University
2017-2024
Fujian Medical University
2023-2024
Osteosarcoma is the most frequent primary bone tumor with poor prognosis. Through RNA-sequencing of 100,987 individual cells from 7 primary, 2 recurrent, and lung metastatic osteosarcoma lesions, 11 major cell clusters are identified based on unbiased clustering gene expression profiles canonical markers. The transcriptomic properties, regulators dynamics malignant together their microenvironment particularly stromal immune characterized. transdifferentiation osteoblastic chondroblastic...
Microbiome-gut-brain axis may be involved in the progression of age-related cognitive impairment and relevant brain structure changes, but evidence from large human cohorts is lacking. This study was aimed to investigate associations gut microbiome with based on multi-omics three independent populations.
Objective: Atherosclerosis is closely associated with ischemic stroke, and long noncoding RNA-H19 (lncRNA-H19) might be a potential target for treating atherosclerosis. The present study aimed to investigate the function of lncRNA-H19 in atherosclerosis explore novel therapeutic strategy stroke. Methods: Differentially expressed genes (DEGs) were screened by searching public database. In combination lncRNA-H19-knockout database, lncRNA-H19-mediated gene was retrieved their relationship...
Abstract Systemic inflammation responses have been associated with cancer development and progression. C-reactive protein (CRP), Glasgow prognostic score (GPS), neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte (PLR), lymphocyte-monocyte (LMR), neutrophil-platelet (NPS) shown to be independent risk factors in various types of malignant tumors. This retrospective analysis 162 osteosarcoma cases was performed estimate their predictive value survival osteosarcoma. All statistical analyses...
The aims of this study were to determine the effect curcumin on osteosarcoma (OS) cells due inactivation p-JAK2/p-STAT3 pathway and evaluate prognostic value in OS.We exposed a human OS cell line (MG-63) different concentrations curcumin. Then, we characterized effects MG-63 using assays (cell viability, colony formation, cycle, wound healing, invasion), flow cytometry, Western blot, immunohistochemical analyses, tumor xenograft.The half-maximal inhibitory for at 24 hours was 27.6 µM. number...
Circular RNA (circRNA) is highly expressed in the brain tissue, but its molecular mechanism cerebral ischemia–reperfusion remains unclear. Here, we explored role and underlying mechanisms of circRNA antisense non-coding INK4 locus (circ_ANRIL) oxygen–glucose deprivation reoxygenation (OGD/R)-induced cell injury. The expression circ_ANRIL OGD/R-induced human microvascular endothelial cells (HBMECs) was significantly up-regulated, while that miR-622 down-regulated. Overexpression inhibited...
The overall survival rate for metastatic osteosarcoma hovers around 20%. Responses to second-line chemotherapy, targeted therapies, and immunotherapies have demonstrated limited efficacy in osteosarcoma. Our objective is validate differentially expressed genes signaling pathways between non-metastatic osteosarcoma, employing single-cell RNA sequencing (scRNA-seq) additional functional investigations. We aim enhance comprehension of mechanisms potentially unveil a therapeutic target.
Abstract Background Interleukin-32 (IL-32) is an important factor in innate and adaptive immune responses, which activates the p38MAPK, NF-kappa B AP-1 signaling pathways. Recent reports have highlighted that IL-32 regulated during viral infection humans. Methods Enzyme-linked immunosorbent assays (ELISA) were carried out to detect levels serum samples. Detailed kinetics of transcription mRNA expression protein human cytomegalovirus (HCMV) determined by semi-quantitative RT-PCR western blot,...
Identifying prognostic factors for osteosarcoma (OS) aids in the selection of patients who require more aggressive management. CD133 has been found to be a factor certain tumor types. However, association between expression and prognosis OS remains unknown. In this study, we analyzed with clinical overall survival, further investigated its potential role metastasis vitro. We 65.7% (46/70) samples using immunohistochemistry, it was positively correlated lung by Chi-square test (P=0.002)...
Interferon regulatory factor 7 is an innate immune target for non–antibiotic-based immunotherapy bacterial infection.
Competing endogenous RNAs (ceRNAs) are a newly discovered class of molecular regulators involved in many diseases, especially tumors.Therefore, exploration the potential ceRNA regulatory network regarding occurrence and development pancreatic cancer will provide new theoretical basis for its diagnosis treatment.Based on above background, we applied bioinformatics approach to mine public database The Cancer Genome Atlas (TCGA) performed series subsequent biology assays confirm hypothesis that...
Abstract Background microRNAs (miRNAs) are a group of regulatory RNAs that regulate gene expression by binding to specific sequences on target mRNAs. However, functional identification mRNA targets is usually difficult and time consuming. Here we report hybrid-PCR as new rapid approach screen putative in vitro. Results Fifteen mRNAs for human cytomegalovirus (HCMV) miR-UL112-1, including previously confirmed HCMV IE72, were identified from mRNA-derived cDNAs using hybrid-PCR. Moreover,...
Ischemic stroke is a major cerebrovascular disease resulting from transient or permanent local reduction of cerebral blood flow. Angiogenesis plays an important role in microvascular repair after ischemic stroke. This study aimed at investigating the effect NF-E2-related factor 2 (Nrf2) on angiogenesis mouse endothelial bEnd.3 cells hypoxic environment. We found that Nrf2 expression was temporarily increased hypoxia-induced cells. Knockdown inhibited proliferation, migration, as well tube...
Chronic heart failure (CHF) is closely associated with inflammation and mitochondrial dysfunction in cardiomyocytes. This study attempts to investigate the effects of microRNA-21-3p (miR-21-3p) on macrophage polarization mitophagy CHF. Here we found miR-21-3p was upregulated CHF negatively correlated carnitine palmitoyl transferase 1A (CPT1A). L-palmitoyl (L-PC) exacerbated isoproterenol (ISO)-induced myocardial structural disruption fibrosis rats, which by miR-21-3p. Mechanistically,...
Human cytomegalovirus (HCMV) is a herpesvirus that causes congenital diseases and opportunistic infections in immunocompromised individuals. Its functional proteins microRNAs (miRNAs) facilitate efficient viral propagation by altering host cell behavior. The identification of target genes miRNAs an important step the study HCMV pathogenesis. encodes at least 14 miRNAs, including hcmv-mir-UL148D, which resides UL/b' region. hcmv-mir-UL148D only miRNA encoded region; however, its targets...
CD133 and CD44 are commonly used markers of cancer stem cells (CSCs), which characterized by their ability for self-renewal tumorigenicity. However, the clinical value significance in lung metastasis osteosarcoma (OS) remains controversial. The purpose this study was to investigate whether CD133(+) CD44(+) mediates OS. We identified metastatic lesions OS cell lines, next demonstrated were more aggressive sphere formation, migration invasiveness compared with CD44(-) , CD133(-) or cells....