Abstract Nitration of tyrosine in biological conditions represents a pathological event that is associated with several neurodegenerative diseases, such as amyotrophic lateral sclerosis, Parkinson's disease and Alzheimer's (AD). Increased levels nitrated proteins have been reported AD brain CSF, demonstrating the potential involvement reactive nitrogen species (RNS) neurodegeneration this disease. Reaction NO leads to formation peroxynitrite ONOO – , which following protonation, generates...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFerrocenyl iron as a donor group for complexed silver in ferrocenyldimethyl[2.2]cryptand: redox-switched receptor effective waterJulio C. Medina, Timothy T. Goodnow, Maria Rojas, Jerry L. Atwood, Bert Lynn, Angel E. Kaifer, and George W. GokelCite this: J. Am. Chem. Soc. 1992, 114, 26, 10583–10595Publication Date (Print):December 1, 1992Publication History Published online1 May 2002Published inissue 1 December...

Studies of oxidative damage during the progression Alzheimer's disease (AD) suggest its central role in pathogenesis. To investigate levels nucleic acid oxidation both early and late stages AD, multiple base adducts were quantified nuclear mitochondrial DNA from superior middle temporal gyri (SMTG), inferior parietal lobule (IPL), cerebellum (CER) age-matched normal control subjects, subjects with mild cognitive impairment, preclinical late-stage non-AD neurological disorders (diseased...

Lignin is a crucial component of plant matter; however, it also largely responsible for the recalcitrance lignocellulosic biomass when subjected to pretreatment processes. generated in large volumes as waste product from paper and pulping industry well cellulosic biorefineries. As result, lignin valorization critical successful implementation biofuels. To this end, we investigated interactions between three ionic liquids (ILs) lignolytic enzyme laccase toward biocatalytic conversion aromatic...

Abstract Ubiquitin carboxyl‐terminal hydrolase L‐1 (UCH L‐1) is a crucial enzyme for proteasomal protein degradation that generates free monomeric ubiquitin. Our previous proteomic study identified UCH as one specific target of oxidation in Alzheimer's disease (AD) brain, establishing link between the effect oxidative stress on and dysfunction AD. However, it unclear how affects function, owing to different responses proteins oxidation. Analysis systems which oxidized displays lowered or...

Acrolein is a highly reactive α,β-unsaturated aldehyde and known to react with DNA forming exocyclic acrolein−deoxyguanosine adducts (Acro−dG). These aldehyde−DNA lesions may play role in mutagenesis, carcinogenesis, neurodegenerative diseases. In the present work, we described development evaluation of sensitive selective capillary liquid chromatography nanoelectrospray isotope dilution tandem mass spectrometry method for quantitatively analyzing Acro−dG hydrolysates. This was achieved by...

Defects in assembly are suggested to signal the dissociation of faulty splicing complexes. A yeast genetic screen was performed identify components putative discard pathway. Weak mutant alleles SPP382 (also called NTR1) were found suppress defects two proteins required for spliceosome activation, Prp38p and Prp8p. Spp382p is shown necessary cellular splicing, with premRNA and, some alleles, excised intron, accumulating after inactivation. Like spp382-1, a allele AAR2 identified this...

A two-dimensional liquid chromatography separation scheme coupled to tandem mass spectrometry (2-D LC−MS/MS) was utilized profile the proteome of human CSF. Ventricular CSF samples acquired post-mortem from 10 cognitively normal elderly subjects (mean ± SEM Braak stage = 1.7 0.2) were analyzed determine their protein composition. Raw subjected an immuno-based processing method remove highly abundant albumin and immunoglobulin (Ig), allowing better detection lower-abundance proteins. Samples...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBrevetoxin B: Chemical Modifications, Synaptosome Binding, Toxicity, and an Unexpected Conformational EffectKathleen S. Rein, Bert Lynn, Robert E. Gawley, Daniel G. BadenCite this: J. Org. Chem. 1994, 59, 8, 2107–2113Publication Date (Print):April 1, 1994Publication History Published online1 May 2002Published inissue 1 April 1994https://pubs.acs.org/doi/10.1021/jo00087a028https://doi.org/10.1021/jo00087a028research-articleACS PublicationsRequest...

A sensitive and selective capillary liquid chromatography nanoelectrospray isotope dilution mass spectrometric method was developed to identify quantify the endogenous cyclic DNA adducts derived from trans-4-hydroxy-2-nonenal with 2'-deoxyguanosine (HNE-dG) in human brain tissues. Authentic 13C 15N stable isotope-labeled HNE-dG were synthesized serve as standards. The vitro HNE-modified calf-thymus well samples isolated tissues of normal Alzheimer's disease subjects enzymatically digested...

Previous epidemiologic studies suggest that antihypertensive drugs may be protective against cognitive decline. To determine if subjects enrolled in the University of Kentucky longitudinal aging study who used showed diminished progression to dementia, we a 3-parameter logistic regression model compare rate dementia for any five common categories those with similar demographic characteristics but did not use antihypertensives. Regression modeling calcium channel blockers (CCBs) other classes...

Converting lignin to value-added products at high yields provides an avenue for making ethanol biorefineries more profitable while reducing the carbon footprint of generally derived from petroleum. In this study, corn stover was depolymerized by catalytic transfer hydrogenolysis (CTH) in supercritical with a Ru/C catalyst. The lignin-derived bio-oil then sequentially extracted utilizing hexane, petroleum ether, chloroform, and ethyl acetate as solvents order less polar polar, subsequent...