A5065379475- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Connexins and lens biology
NIHR Great Ormond Street Hospital Biomedical Research Centre
2021
Great Ormond Street Hospital
2019-2021
University College London
2019-2021
Organoid cultures represent a unique tool to investigate the developmental complexity of tissues like human retina. NRL is transcription factor required for specification and homeostasis mammalian rod photoreceptors. In Nrl-deficient mice, photoreceptor precursor cells do not differentiate into rods, instead follow default pathway generate S-cone-like cells. To whether this genetic switch mechanism conserved in humans, we used CRISPR/Cas9 gene editing engineer an NRL-deficient embryonic stem...
Abstract Irreversible photoreceptor cell death is a major cause of blindness in many retinal dystrophies. A better understanding the molecular mechanisms underlying progressive loss cells remains therefore crucial. Abnormal expression microRNAs (miRNAs) has been linked with aetiology number However, their role during degenerative process poorly understood. Loss cone photoreceptors human macula greatest impact on sight as these provide high acuity vision. Using Chrnb4-cre; Dicer flox/flox...