A5004863273- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Insect behavior and control techniques
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Insect and Pesticide Research
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Genetic and Mutation Studies
- Plant Pathogens and Fungal Diseases
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Agricultural pest management studies
- Mycorrhizal Fungi and Plant Interactions
- Insect Pest Control Strategies
- Growth and nutrition in plants
- Genetic diversity and population structure
- Banana Cultivation and Research
- Papaya Research and Applications
- Genetically Modified Organisms Research
- Plant biochemistry and biosynthesis
- Agricultural Economics and Practices
Indian Institute of Horticultural Research
2014-2025
Indian Council of Agricultural Research
2020-2024
Chhattisgarh Kamdhenu Vishwavidyalaya
2024
GVK (India)
2024
In-Q-Tel
2004
RNA interference (RNAi) is a potential tool for functional characterization of genes and also in the management insect pests. Accumulated literature reveals that RNAi efficiency varies among species reported to be less efficient lepidopteran insects.We attempted enhance by concatemerizing short double-stranded (dsRNA) sequence. Then effectiveness concatemerized dsRNAs (C-dsRNAs) was compared with non-concatemerized long dsRNA (NCL-dsRNA) silencing acetylcholinesterase (AChE) diamondback...
Abstract The Oriental fruit fly, Bactrocera dorsalis (Hendel), is a highly invasive pest of quarantine importance affecting the global trade. In managing B. , methods like cultural, biological, chemical, sterile insect technique (SIT), and semiochemical‐mediated attract‐and‐kill are in use with varying success. SIT approach method choice for chemical‐free, long‐term suppression followed many countries across globe. nonspecific mutations caused by irradiation affect overall fitness flies,...
Insects have a rich diversity of RNA viruses that can either cause acute infections or persist in host populations without visible symptoms. The melon fly, Zeugodacus cucurbitae (Tephritidae) causes substantial economic losses through infestation diverse cucurbit and other crops. Of Indomalayan origin, it is now established many tropical regions the world. virome Z. largely unknown across large parts its distribution, including Indian subcontinent. We analysed three transcriptomes each one...
ABSTRACT The Oriental fruit fly, Bactrocera dorsalis ( B. ) is a highly invasive, widely distributed notorious pest restricting global trade immensely. There are several approaches to managing this pest, still require newer approaches. In regard, recently novel approach called precision‐guided sterile insect technique (pgSIT) gaining momentum in inducing both female sex elimination or conversion and male sterility at one go. Developing species‐specific pgSIT system requires validation of...
Abstract Ribonucleic acid interference (RNAi) is a sequence-specific gene silencing mechanism induced by double-stranded RNA (dsRNA). Recently, RNAi has gained popularity as reverse genetics tool owing to its tremendous potential in insect pest management, which includes Helicoverpa armigera . However, efficiency mainly governed dsRNA concentration, frequency of application, target gene, etc. Therefore, obtain robust response H. , we evaluated various concentrations and applications...
Identification of novel approaches for managing the global pest, Fall armyworm, Spodoptera frugiperda, is need hour, as it defies many management strategies including synthetic chemicals, Bt transgenics, and so on. Recently CRISPR/Cas9-based genome editing opened up newer avenues to design pest such precision-guided sterile insect technique (pgSIT). In this regard, genes governing sex determination, egg reproduction, spermatogenesis could be prime targets editing. This requires validation...
Invasive insect pests, currently, pose a serious economic threat to several staple crops all over the world, one such being fall armyworm, Spodoptera frugiperda. It was first observed in Africa since 2016, outside of its natural habitat Americas. Subsequently, it invaded countries South and East Asia also very recently Australia. In newly regions, maize is principal crop attacked causing concern poor farmers, particularly developing countries. Owing innate genetic ability, defies many...
Abstract Pest management based on CRISPR/Cas9-mediated site-specific mutations is an effective and environmentally safer strategy to suppress the pest population. However, potential of this approach yet be tested many important agricultural pests such as Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), a fit candidate for area-wide management. Therefore, in present study, 2 spermatogenesis-related genes viz. Testis-specific zinc finger protein (topi) serine kinase 1 (Tssk1) B. were...
Abstract Molecular diversity of genes, namely actin, glutathione-S-transferase (gst), cytochrome P450, chymotrypsin and serine protease from the interspecific populations fruit borer Helicoverpa armigera was studied to understand sequence polymorphism. These genes were cloned, sequenced analysed employing editor BioEdit MEGA 4.0. Sequence comparisons showed variation as follows: Actin – 35 positions, 3.09%, gst 60 9.66%, P450 64 8.11%, 36 4.02%, 119 15.31%. Phylogenetic analysis concluded...
Abstract The oriental fruit fly ( Bactrocera dorsalis ) is an exceptionally hazardous pest that can cause up to 100% loss. Male annihilation using male attractant methyl eugenol (ME) the most adopted control measure of this insect. molecular process underlying perception insects remains unexplored. In study, we focus on gene functionality odorant‐binding protein 13 (OBP13) in ME by knockout CRISPR/Cas9 mediated embryonic editing through microinjection. electrophysiological study mutant flies...
Helicoverpa armigera (Hubner) is a polyphagous pest causing severe yield loss in many important crops. Host plants produce allelochemicals to deter insect pests and response, insects deploy cytochrome P450 monooxygenases (P450s) detoxify allelochemicals. Understanding the response of P450s allelochemical exposure key effective management. We studied seven H. isoforms different concentrations three allelochemi-cals (gossypol, tomatine xanthotoxin) their effects on growth survival....