Regina Kleespies

ORCID: 0000-0001-8549-9928
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Research Areas
  • Entomopathogenic Microorganisms in Pest Control
  • Insect Resistance and Genetics
  • Insect symbiosis and bacterial influences
  • Insect and Pesticide Research
  • Forest Insect Ecology and Management
  • Viral Infectious Diseases and Gene Expression in Insects
  • Insect-Plant Interactions and Control
  • Insect behavior and control techniques
  • Insect Pest Control Strategies
  • Plant Virus Research Studies
  • Plant and fungal interactions
  • Nematode management and characterization studies
  • Entomological Studies and Ecology
  • Plant Pathogens and Fungal Diseases
  • Genomics and Phylogenetic Studies
  • Insect and Arachnid Ecology and Behavior
  • Insects and Parasite Interactions
  • Parasitic Infections and Diagnostics
  • Parasite Biology and Host Interactions
  • Identification and Quantification in Food
  • Insect Utilization and Effects
  • Biological Control of Invasive Species
  • Bacteriophages and microbial interactions
  • Vector-borne infectious diseases
  • Viral Infections and Vectors

Julius Kühn-Institut
2014-2024

Institut für biologische Forschung
2008

HUN-REN Szegedi Biológiai Kutatóközpont
1995

Abstract According to the Convention on Biological Diversity, by 2020 invasive alien species (IAS) should be identified and their impacts assessed, so that can prioritized for implementation of appropriate control strategies measures put in place manage invasion pathways. For one quarter IAS listed as “100 world's worst” environmental are linked diseases wildlife (undomesticated plants animals). Moreover, a significant source “pathogen pollution” defined human‐mediated introduction pathogen...

10.1111/conl.12297 article EN cc-by Conservation Letters 2016-08-25

Abstract Rapid and reliable detection of pathogens is crucial to complement the growing industry mass-reared insects, in order safeguard insect colonies from outbreak diseases, which may cause significant economic loss. Current diagnostic methods are mainly based on conventional PCR microscopic examination, requiring prior knowledge disease symptoms limited identifying known pathogens. Here, we present a rapid nanopore-based metagenomics approach for detecting entomopathogens European house...

10.1038/s41598-024-58768-3 article EN cc-by Scientific Reports 2024-04-12

Studies on blastospore production in different liquid media were conducted with three strains of Metarhizium anisopliae var. (M. a.) derived from various countries a. 43: Austria, M. 57: Brazil, 97: Philippines). Variation six fermentation parameters (cornsteep products, carbohydrates, pH values, temperature, Tween 80, and polyethyleneglycol (PEG) 200) disclosed that the differed their growth pattern physiology. In standard medium all tests, 57 produced highest number blastospores invariably...

10.1080/09583159209355226 article EN Biocontrol Science and Technology 1992-01-01

The Gryllus bimaculatus nudivirus (GbNV) infects nymphs and adults of the cricket (Orthoptera: Gryllidae). GbNV other nudiviruses such as Heliothis zea 1 (HzNV-1) Oryctes rhinoceros (OrNV) were previously called "nonoccluded baculoviruses" they share some similar structural, genomic, replication aspects with members family Baculoviridae. Their relationships to each baculoviruses are elucidated by sequence complete genome GbNV, which is 96,944 bp, has an AT content 72%, potentially contains...

10.1128/jvi.02781-06 article EN Journal of Virology 2007-03-15

Generating complete, high-quality genome assemblies is key for any downstream analysis, such as comparative genomics. For bacterial assembly, various algorithms and fully automated pipelines exist, which are free-of-charge easily accessible. However, these assembly tools often cannot unambiguously resolve a genome, example due to the presence of sequence repeat structures on chromosome or plasmids. Then, more sophisticated approach and/or manual curation needed. Such modifications can be...

10.1099/mgen.0.001173 article EN cc-by Microbial Genomics 2024-01-10

Hard ticks (Acari: Ixodidae) are known to harbour intracellular bacteria from several phylogenetic groups that can develop both mutualistic and pathogenic relationships the host. This is of particular importance for public health as tick derived potentially be transmitted mammals, including humans, where e.g. Rickettsia or Coxiella act severe pathogens. Exact molecular taxonomic identification associated prokaryotes a necessary prerequisite investigation their relationship possible...

10.1371/journal.pone.0038062 article EN cc-by PLoS ONE 2012-05-31

Bacillus thuringiensis subsp. tenebrionis (Btt) produces a coleopteran-specific crystal protoxin protein (Cry3Aa δ-endotoxin). After its discovery in 1982, the strain NB125 (DSM 5526) was eventually registered 1990 to control Colorado potato beetle (Leptinotarsa decemlineata). Gamma-irradiation of resulted NB176-1 5480) that exhibited higher cry3Aa production and became active ingredient plant protection product Novodor® FC. Here, we report comparative genome analysis parental NB125,...

10.3389/fcimb.2023.1129177 article EN cc-by Frontiers in Cellular and Infection Microbiology 2023-03-20

The iridovirus isolate termed cricket (CrIV) was isolated in 1996 from Gryllus campestris L. and Acheta domesticus (both Orthoptera, Gryllidae). CrIV DNA shows distinct restriction patterns different those known for Insect iridescent virus type 6 (IIV-6). This observation led to the assumption that might be a new species within family Iridoviridae. can transmitted perorally orthopteran species, resulting specific, fatal diseases. These include bimaculatus African migratory locust Locusta...

10.1099/0022-1317-83-2-463 article EN Journal of General Virology 2002-02-01

The genus Rickettsiella (class Gammaproteobacteria; order Legionellales; family Coxiellaceae) comprises intracellular bacterial pathogens of a wide range arthropods that are currently classified in the three recognized species, popilliae, grylli, and chironomi. bacteria contain type IVB secretion system (T4SS) known to be key virulence factor related Legionella. Providing first respective sequence information for nomenclatural R. T4SS components DotA, IcmB, IcmQ were used as phylogenetic...

10.1111/j.1574-6968.2007.01025.x article EN FEMS Microbiology Letters 2008-02-01

Studies on the blastospore production of Metarhizium anisopliae var. were conducted in Adamek's medium used as a standard, enriched with lecithin, collagen, lactic acid or polyethylene glycol 200 (PEG 200) to increase spore yield and suppress mycelial pellet formation. The addition 5% lecithin resulted significant 10-fold up 1.9 108 blastospores/ml compared 107 spores/ml standard medium. Collagen (3%) increased number blastospores 3.7-fold, (1.5%) two-fold. A reduction formation favour was...

10.1080/09583159830270 article EN Biocontrol Science and Technology 1998-06-01

'Rickettsiella melolonthae' is an intracellularly multiplying bacterial pathogen of European cockchafers, Melolontha melolontha (Linnaeus, 1758) and hippocastani (Fabricius, 1801) (Coleoptera: Scarabaeidae). We report the first determination nucleotide sequences from this organism, i.e. 16S rRNA encoding rrs gene, chaperonin groEL gene mucZ orthologue a capsule synthesis-inducing factor Coxiella burnetii. Within genus Rickettsiella, pathotype currently classified as synonym nomenclatural...

10.1099/ijs.0.65359-0 article EN INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 2008-04-01
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