A5053081524- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Oral and Maxillofacial Pathology
- Proteoglycans and glycosaminoglycans research
- Monoclonal and Polyclonal Antibodies Research
- Signaling Pathways in Disease
- Animal Genetics and Reproduction
- Toxin Mechanisms and Immunotoxins
- Connexins and lens biology
- Xenotransplantation and immune response
- dental development and anomalies
- Cell Adhesion Molecules Research
- Bone and Dental Protein Studies
- RNA Interference and Gene Delivery
- Retinoids in leukemia and cellular processes
- Enzyme Production and Characterization
- Salivary Gland Tumors Diagnosis and Treatment
- Bone Tumor Diagnosis and Treatments
- Coronary Interventions and Diagnostics
- Tumors and Oncological Cases
- Endodontics and Root Canal Treatments
- Galectins and Cancer Biology
- Infant Nutrition and Health
- Peptidase Inhibition and Analysis
- Head and Neck Surgical Oncology
Tokyo Dental College
2016-2025
Fujita Health University Hospital
2023-2025
Fujita Health University Okazaki Medical Center
2024
Fujita Health University
2022
Shimadzu (Japan)
2021
NYU Langone’s Laura and Isaac Perlmutter Cancer Center
2021
Mitsubishi Heavy Industries (Japan)
2011-2019
Tokyo Medical University Hospital
2019
Nagoya University
2004-2016
Tsurumi University
2012-2016
Using [3H]-labeled oligosaccharides, we found that the presence of at least two αmannosyl residues with free hydroxyl groups C-3, 4, and 6 is required for oligosaccharides to be retained by a concanavalin A-Sepharose column. This finding also applicable N-[14C]acetylated glycopeptides. Thus, column might become useful tool structural studies glycopeptides their fractionation. Glycopeptides prepared from trypsinate rat fibroblasts, which had been purified paper electrophoresis, were further...
Abstract Vascular endothelial growth factor (VEGF) plays a critical role during normal embryonic angiogenesis and also in the pathological that occurs number of diseases, including cancer. We developed novel VEGF blockade system using RNA interference. The small interfering (siRNA) targeting human almost completely inhibited secretion prostate cancer cell line, PC-3, whereas control scramble siRNA showed no effects. with atelocollagen dramatically suppressed tumor PC-3 s.c. xenograft model....
Abstract A purified ovalbumin glycopeptide, Asn-(GlcNAc)2(Man)5 was [14C]acetylated in the asparagine moiety. Using glycopeptide as a substrate, an endo-β-N-acetylglucosaminidase 2400-fold from culture fluid of Diplococcus pneumoniae. The enzyme preparation practically free various exoglycosidases and proteases. cleaved di-N-acetylchitobiose structure yielding equimolar amounts [14C]acetyl-Asn-GlcNAc (Man)5(GlcNAc). had pH optimum 6.5, with Km 0.25 mm Vmax 4.67 µmoles per mg protein min...
Background: Body fat percentage (BF%) determined by bioelectrical impedance analysis is widely used at home and in medical check-ups. However, the clinical significance of measuring BF% has not been studied detail. Methods Results: A cross-sectional study was carried out on a cohort 10,774 middle-aged Japanese men who had undergone an annual check-up 2008. Cut-off points were evaluated for body mass index (BMI), waist circumference (WC), detecting participants with cardiovascular disease...
A newly identified gene MK is transiently expressed in early stages of retinoic acid-induced differentiation embryonal carcinoma cells (Kadomatsu, K., M. Tomomura, and T. Muramatsu, 1988. Biochem. Biophys. Res. Commun. 151:1312-1318). has been predicted to code a polypeptide that rich basic amino acids cysteine not related any other peptides so far reported. In the present study, we investigated expression during mouse embryogenesis by situ hybridization. The transcript was detected all over...
The neural retina expresses multiple monocarboxylate transporters (MCTs) that are likely to play a key role in the metabolism of outer retina. Recently, it was reported targeting MCT1 and -4 plasma membrane requires association with 5A11/basigin (CD147). In present study, hypothesis reduced amplitudes electroretinograms null mouse (Bsg(-/-)) may be linked altered expression MCTs studied.The subcellular distribution Bsg(-/-) mice analyzed by immunofluorescence microscopy isoform-specific...
From embryonal carcinoma cells labeled with fucose, two main classes of glycopeptide products Pronase digestion can be distinguished by Sephadex G-50 column chromatography: one eluted near the excluded volume and a smaller one. The large fucosyl-glycopeptides are scarcely present in differentiated derived from (i.e., fibroblastlike cells, myoblasts, parietal yolk-sac carcinoma). During vitro differentiation these glycopeptides disappear almost completely. small were analyzed paper...
A newly identified gene, MK, is transiently expressed in the early stages of embryonal carcinoma cell differentiation and mid-gestation period mouse embryogenesis (Kadomatsu, K., Tomomura, M., Muramatsu, T. (1988) Biochem. Biophys. Res. Commun. 151, 1312-1318). Analysis various MK cDNA clones revealed differences 5'-region. So far three classes (MK1, MK2, MK3) have been identified; they were different 5'-untranslated region but shared rest sequence. Ribonuclease protection, RNA blotting,...
The distribution of binding sites for two lectins with different specificities was studied in adult mice by staining paraffin sections labeled either fluorescein isothiocyanate or horseradish peroxidase. Binding Dolichos biflorus agglutinin, a lectin specific to terminal alpha-N-acetylgalactosamine residue, were detected only several restricted regions, such as collecting tubules and Bowman's capsules the kidney, bile ducts, pancreatic sperms, oocytes, some secreting cells, secreted mucin...
Mechanisms underlying coffee's beneficial actions against cardiovascular disease and glucose metabolism are not well understood. Little information is available regarding association between coffee consumption adipocytokines.We investigated potential associations adiponectin, leptin, markers for subclinical inflammation, metabolism, lipids liver enzymes. We then whether adipocytokines played a role in the these markers.This cross-sectional study comprising 2554 male 763 female Japanese...
Midkine (MK) is a new member of the heparin-binding neurotrophic factor family. MK plays important roles in development and carcinogenesis has several biological effects, including promotion neurite extension neuronal survival. However, mechanism by which exerts its actions on neurons not been elucidated to date. We have established an apoptosis induction system serum deprivation primary cultures isolated from mouse cerebral cortices. Neuronal induced was accompanied activation caspase-3....
Basigin belongs to the immunoglobulin superfamily and may be related primordial form of superfamily. Human basigin cDNA was isolated sequenced, predicted protein structure compared with that mouse two molecules, embigin chicken blood-brain barrier antigen HT7. Between human basigin, 58% amino acids were identical 80% changes conservative. A stretch 29 acid residues in transmembrane cytoplasmic domains conserved not only but also HT7 antigen. The required for interaction a membranous protein....
Background : Midkine (MK) is a growth factor implicated in the development and repair of various tissues, especially neural tissues. However, its vivo function has not been clarified. Results Knockout mice lacking MK gene ( Mdk ) showed no gross abnormalities. We closely analysed postnatal brain (–/–) using calcium binding proteins as markers to distinguish neuronal subpopulations. Intense prolonged calretinin expression was found dentate gyrus granule cell layer hippocampus infant mice. In...
Abstract Midkine (MK) is the product of a retinoic acid responsive gene and member new family heparin‐binding growth factors. Neurotrophic effects MK were examined using cultured spinal cord dorsal root ganglion (DRG) neurons derived from fetal mouse. MK, which was added to culture medium at concentrations 1–100 ng/ml, promoted survival both types approximately 5‐fold after 7 days in culture. For neurons, increased reflected an increase choline acetyltransferase activity. also neurite...