A5113622972- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Phytase and its Applications
- Plant Reproductive Biology
- Transgenic Plants and Applications
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Redox biology and oxidative stress
- Seed Germination and Physiology
- Plant Micronutrient Interactions and Effects
- Endoplasmic Reticulum Stress and Disease
- Pineapple and bromelain studies
- Heat shock proteins research
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- GABA and Rice Research
- Biochemical and Structural Characterization
- Enzyme Structure and Function
- Enzyme Production and Characterization
- Ubiquitin and proteasome pathways
- Fungal and yeast genetics research
- Plant biochemistry and biosynthesis
Gyeongsang National University
2013-2023
Pusan National University
2008
University of Missouri
2008
University of Georgia
2008
Biotechnology Research Center
1997-2003
Korea Research Institute of Bioscience and Biotechnology
1999-2002
Catholic University of Pusan
2002
Korea Advanced Institute of Science and Technology
2002
Dong-A University
2002
Chinju National University of Education
1992
NDP kinases (NDPKs) are multifunctional proteins that regulate a variety of eukaryotic cellular activities, including cell proliferation, development, and differentiation. However, much less is known about the functional significance NDPKs in plants. We show here NDPK associated with H 2 O -mediated mitogen-activated protein kinase signaling stress strongly induces expression NDPK2 gene Arabidopsis thaliana ( AtNDPK2 ). Proteins from transgenic plants overexpressing showed high levels...
Abstract The Ca2+-binding protein calmodulin mediates cellular Ca2+ signals in response to a wide array of stimuli higher eukaryotes. Plants express numerous CaM isoforms. Transcription one soybean (Glycine max) isoform, SCaM-4, is dramatically induced within 30 min pathogen or NaCl stresses. To characterize the cis-acting element(s) this gene, we isolated an approximately 2-kb promoter sequence gene. Deletion analysis revealed that 130-bp region located between nucleotide positions −858 and...
Cold stress on plants induces changes in the transcription of cold response genes. A cDNA clone encoding C2H2-type zinc finger protein, SCOF-1, was isolated from soybean. The SCOF-1 is specifically induced by low temperature and abscisic acid (ABA) but not dehydration or high salinity. Constitutive overexpression cold-regulated (COR) gene expression enhanced tolerance non-acclimated transgenic Arabidopsis tobacco plants. localized to nucleus did bind directly either C-repeat/dehydration...
Calmodulin (CaM), a ubiquitous calcium-binding protein, regulates diverse cellular functions by modulating the activity of variety enzymes and proteins. Plants express numerous CaM isoforms that exhibit differential activation and/or inhibition CaM-dependent in vitro. However, specific biological plant are not well known. In this study, we isolated cDNA encoding binding transcription factor, MYB2, expression salt- dehydration-responsive genes Arabidopsis. This was achieved using...
Mitogen-activated protein kinase (MAPK) cascades are known to transduce plant defense signals, but the downstream components of MAPK have as yet not been elucidated. Here, we report an from rice (Oryza sativa), BWMK1, and a transcription factor, OsEREBP1, phosphorylated by kinase. The carries TDY phosphorylation motif instead more common TEY in its domain has unusually extended C-terminal that is essential activity translocation nucleus. phosphorylates OsEREBP1 binds GCC box element...
Calmodulin (CaM) is a ubiquitous Ca 2+ ‐binding protein known to regulate diverse cellular functions by modulating the activity of various target proteins. We isolated cDNA encoding AtWRKY7, novel CaM‐binding transcription factor, from an Arabidopsis expression library with horseradish peroxidase‐conjugated CaM. CaM binds specifically ‐dependent domain (CaMBD) as shown site‐directed mutagenesis, gel mobility shift assay, split‐ubiquitin and competition assay using /CaM‐dependent enzyme....
In order to study molecular interactions that occur between rice and blast fungus upon infection, we isolated fungal elicitor-responsive genes from (Oryza sa-tiva cv. Milyang 117) suspension-cultured cells treated with elicitor prepared the (Magnaporthe grisea) employing a method combined mRNA differential display cDNA library screening. Data base searches clones revealed OsERG1 OsERG2 cDNAs share significant similarities mammalian Ca 2+ -dependent lipid binding (C2) domains. The OsCPX1 is...
Calmodulin (CaM) regulates diverse cellular functions by modulating the activities of a variety enzymes and proteins. However, direct modulation transcription factors CaM has been poorly understood. In this study, we isolated putative factor screening rice cDNA expression library using CaM:horse-radish peroxidase as probe. This factor, which have designated OsCBT (Oryza sativa CaM-binding factor), structural features similar to Arabidopsis AtSRs/AtCAMTAs encodes 103-kDa protein because it...
The H 2 O ‐catabolizing peroxidase activity of human peroxiredoxin I (hPrxI) was previously shown to be regulated by phosphorylation Thr 90 . Here, we show that hPrxI forms multiple oligomers with distinct secondary structures. HPrxI is a dual function protein, since it can behave either as or molecular chaperone. effects on its protein structure and functions were determined using site‐directed mutagenesis, in which the site substituted aspartate mimic phosphorylated status (T90D‐hPrxI)....
Calmodulin (CaM), a ubiquitous calcium-binding protein, regulates diverse cellular functions by modulating the activity of variety proteins. However, little is known about how CaM directly transcription. Screening an Arabidopsis cDNA expression library using horseradish peroxidase-conjugated calmodulin as probe identified calmodulin-binding NAC protein (CBNAC). Using gel overlay assays, Ca2+-dependent CaM-binding domain was in C terminus this protein. Specific binding to corroborated...
Dehydration-responsive element-binding proteins (DREBs)regulate plant responses to environmental stresses. In the current study, transcription of DREB2C, a class 2 Arabidopsis DREB, was induced by superoxide anion propagator, methyl viologen (MV). The oxidative stress tolerance DREB2C-overexpressing transgenic plants significantly greater than that wild-type plants, as measured ion leakage and chlorophyll fluorescence under light conditions. transcriptional activity several ascorbate...
Heat shock factors (Hsfs) are central regulators of abiotic stress responses, especially heat in plants. In the current study, we characterized activity Hsf gene HsfA3 Arabidopsis under oxidative conditions. transcription seedlings was induced by reactive oxygen species (ROS), exogenous hydrogen peroxide (H 2 O ), and an endogenous H propagator, 2,5dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB). HsfA3-overexpressing transgenic plants exhibited increased tolerance compared to...
Ca2+-ATPases are key regulators of Ca2+ ion efflux in all eukaryotes. Animal cells have two distinct families pumps, with calmodulin-stimulated pumps (type IIB pumps) found exclusively at the plasma membrane. In plants, no equivalent type pump located membrane has been identified molecular level, although related isoforms non–plasma locations. Here, we identify a plant cDNA, designated SCA1 (for soybean Ca2+-ATPase 1), that encodes and is The localization was determined by sucrose gradient...
Abstract LONG HYPOCOTYL5 (HY5) is a bZIP (basic leucine zipper) transcription factor that activates photomorphogenesis and root development in Arabidopsis (Arabidopsis thaliana). Previously, STF1 (soybean [Glycine max] TGACG-motif binding 1), homologous legume protein with RING-finger motif domain, was reported soybean. To investigate the role of STF1, phenotypes transgenic plants overexpressing HY5 were compared. In addition, DNA-binding properties extensively studied using random site...
The phytocystatins of plants are members the cystatin superfamily proteins, which potent inhibitors cysteine proteases. Arabidopsis genome encodes seven phytocystatin isoforms (AtCYSs) in two distantly related AtCYS gene clusters. We selected AtCYS1 and AtCYS2 as representatives for each cluster then generated transgenic expressing GUS reporter under control promoter. These were used to examine expression at various stages plant development response abiotic stresses. Histochemical analysis...
Many plant hormones are involved in coordinating the growth responses of plants under stress. However, not many mechanistic studies have explored how maintain balance between and stress responses. Brassinosteroids (BRs), plant-specific steroid hormones, affect aspects development over a plant's lifetime. In this study we determined that exogenous treatment BR helped overcome cold condition only when pretreated with less than 1 nM, brassinosteroid-insensitive (bri1) mutation, which results...
Possible functions that have been proposed for the plant 1Cys‐peroxiredoxin, include activity as a dormancy regulator and an antioxidant. The transcript level of ice ys‐ eroxi edo in ( R1C‐Prx ) rapidly decreased after imbibition rice seeds, but protein was detected 15 days imbibition. To investigate function this protein, we generated transgenic tobacco plants constitutively expressing gene. showed germination frequency similar to control plants. However, lines exhibited higher resistance...