A5080681841- Heavy metals in environment
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Wastewater Treatment and Reuse
- Heavy Metals in Plants
- Protein Hydrolysis and Bioactive Peptides
- Phytase and its Applications
- Heavy Metal Exposure and Toxicity
- Plant Micronutrient Interactions and Effects
- Cancer and Skin Lesions
- Fish Biology and Ecology Studies
- Aquaculture Nutrition and Growth
- Mitochondrial Function and Pathology
- Agricultural Science and Fertilization
- Genetic Neurodegenerative Diseases
- Microbial Metabolites in Food Biotechnology
- Global Maternal and Child Health
- Gastric Cancer Management and Outcomes
- Plant-Microbe Interactions and Immunity
- Genetic and rare skin diseases.
- Alzheimer's disease research and treatments
- Advanced Drug Delivery Systems
- Livestock Management and Performance Improvement
University of Sargodha
2015-2025
University of Central Punjab
2018-2024
University of Agriculture Faisalabad
2021-2024
University of Kashmir
2023
University of Faisalabad
2023
Northwest A&F University
2023
National University of Medical Sciences
2022
Sher-i-Kashmir Institute of Medical Sciences
2021
University of the Punjab
2019-2021
University of Lahore
2019-2021
HIV/AIDS has emerged as a serious public health issue across the globe, and particularly in developing countries. Comprehensive knowledge positive attitudes are cornerstones for prevention, control treatment of HIV/AIDS. However, there various misconceptions associated with transmission, which lead to negative towards people living AIDS. The present study aims explore effects these determinants, related socio-demographic characteristics autonomy, on women's overall regarding Pakistan.
Nickel (Ni) is essential for plant metabolism but dangerous to most plants and other organisms when present in large concentrations. In this study, nickel was chosen as an important environmental pollutant toxic substance due its widespread availability, high persistence, causing soil pollution serious problems. The main aim of the study determine Ni content various wheat varieties grown water regimes (sewage water, groundwater industrial water) Punjab, Pakistan. concentrations were...
Abstract Present study concerned with expression and biochemical characterization of lipase enzyme for potential use in detergent formulation. Lipase gene (1242 bp) Bacillus thuringensis was cloned expressed Escherichia coli BL21 strain using pET‐21a(+) vector. Maximum obtained at 37°C an induction 0.4 mM IPTG (Isopropyl ß‐D‐1‐thiogalactopyranoside) after 4 h induction. Recombinant purified to homogeneity immobilized metal ion affinity chromatography carrying 109.80 U/mg specific activity...
A low temperature alkali (LTA) pretreatment method was used to treat wheat straw.
The present study describes the cloning, expression, purification and characterization of a xylosidase gene from Clostridium clariflavum into E. coli BL21 (DE3) using expression vector pET-21a(+) for utilization in biofuel production.
Various field trials were performed at different locations of Punjab, Pakistan to study the effect trace metals on uptake iron (Fe) and wheat productivity as well health risk assessment by ingestion grains grown with application irrigation sources. The experimental design was laid out in RCBD having four replications. Soil samples taken before sowing crop from 0 15 cm depth Triticum astivum after through water treatments examined for pollution load index (PLI), bioaccumulation factor (BAF),...
Background: Bronchiectasis is a chronic respiratory disorder characterized by irreversible bronchial dilation, impaired mucociliary clearance, and recurrent infections, leading to progressive pulmonary dysfunction. Airway clearance techniques play crucial role in managing symptoms improving function. The active cycle of breathing technique (ACBT) widely used physiotherapy intervention, often combined with postural drainage (PD) or autogenic (AD). However, comparative evidence regarding their...
This research tries to quantify awareness, integration, and uptake of genetic ideas in Type 2 Diabetes (T2D) management by Punjab general practitioners (GPs). The used a quantitative design, whereby self-report questionnaire was collect data from 110 GPs via snowball sampling. explores three main aims: (1) measure the awareness with regard cause T2D, (2) extent which considerations are incorporated into T2D management, (3) determine predictors information management. Descriptive statistics,...
Soil pH is a critical characteristic that regulates the abundance of essential nutrients in soil system. Low reduces supply secondary macronutrients, whereas higher restricts micronutrient availability. In addition, nutrient sources such as organic and inorganic fertilizers by plants require an adequate for optimum plant growth productivity. The regarded "master indices", which has role to play controlling biogeochemical cycles influence growth. It also enormous on microbial biodiversity...
In this study, the industrial applications of a highly thermostable α-amylase as desizer in textile industry was evaluated. The cloned gene expressed different media (ZBM, LB, ZYBM9, and ZB) with IPTG (isopropyl β-d-1-thiogalactopyranoside) used an inducer. Lactose also alternate inducer for T7 promoter system E. coli. For large-scale production enzyme, parameters were optimized. maximum enzyme achieved when volume medium 70% total fermenter 2.0 vvm air supply 20% dissolved oxygen at 200 rpm...
The present study describes the cloning of cellobiohydrolase gene from a thermophilic bacterium Clostridium clariflavum and its expression in Escherichia coli BL21(DE3) utilizing vector pET-21a(+). optimization various parameters (pH, temperature, isopropyl β-d-1-thiogalactopyranoside (IPTG) concentration, time induction) was carried out to obtain maximum enzyme activity (2.78 ± 0.145 U ml-1) recombinant enzyme. obtained at pH 6.0 70 °C respectively. Enzyme purification performed by heat...
The β-xylanase gene (DCE06_04615) with 1041 bp cloned from Thermotoga naphthophila was expressed into E. coli BL21 DE3. covalently bound to iron oxide magnetic nanoparticles coated silica utilizing carbodiimide. size of the immobilized MNPs (50 nm) and their binding were characterized by Fourier-transform electron microscopy (FTIR) (a change in shift particularly C-O C-N) transmission (TEM) (spherical shape 50 nm diameter). results showed that enzyme activity (4.5 ± 0.23 U per mL),...
Huntington's disease is a dominant autosomal inherited neurodegenerative that results in progressive impairment, characterized by dementia, chorea, and behavioral cognitive decline. The objective of this study was to investigate the potential activity metalloproteins against huntingtin protein using various insertion-based engineering computational methods. Metalloproteins, metal complexes involved important biochemical physiological processes, were explored as drug candidates for disease.A...
Thermostable alkaline serine protease gene of Geobacillus stearothermophilus B‐1172 was cloned and expressed in Escherichia coli BL21 (DE3) using pET‐22b(+), as an expression vector. The growth conditions were optimized for maximal production the variable fermentation parameters, i.e., pH, temperature, addition inducer. Protease, thus produced, purified by ammonium sulfate precipitation followed ion exchange chromatography with 13.7‐fold purification, specific activity 97.5 U mg −1 , a...
Background: Wound infection is the cause of mortality and morbidity on a global scale. Microorganisms infecting wounds can multiply colonize in wound, resulting host tissue damage. Objectives: The present study was conducted to identify Proteus mirabilis patients Quetta district. Methods: This from June 2017 2018 at Center for Advanced Studies Vaccinology Biotechnology, University Balochistan, Quetta. Overall, 480 different wound samples were collected admitted Bolan Medical Complex...