A5039032890- Immune Cell Function and Interaction
- Melanoma and MAPK Pathways
- Cancer Mechanisms and Therapy
- Microtubule and mitosis dynamics
- Immunotherapy and Immune Responses
- Advanced Fluorescence Microscopy Techniques
- Brain Metastases and Treatment
- Gold and Silver Nanoparticles Synthesis and Applications
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Nanomaterials for catalytic reactions
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Cancer-related Molecular Pathways
- Platelet Disorders and Treatments
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Hemoglobin structure and function
- Near-Field Optical Microscopy
- Force Microscopy Techniques and Applications
- NF-κB Signaling Pathways
- Cancer therapeutics and mechanisms
- Blood properties and coagulation
- Endoplasmic Reticulum Stress and Disease
Yale Cancer Center
2023-2025
Yale University
2023-2024
University of New Mexico
2017-2022
Peter Doherty Institute
2020-2022
The University of Melbourne
2020-2022
Fundación Ciencia and Vida
2017-2019
Advanced Center for Chronic Diseases
2017
University of Chile
2017
Tissue-resident cells exhibit tissue-specific overlays to the T RM cell residency program that includes flexible FABP isoform selection tailored tissue of lodgment.
Colorectal cancer (CRC) development and progression is associated with chronic inflammation. We have identified the MAPK-activated protein kinase 2 (MK2) pathway as a primary mediator of inflammation in CRC. MK2 signaling promotes production proinflammatory cytokines IL-1β, IL-6 TNF-α. These been implicated tumor growth, invasion metastasis. For first time, we investigate whether inhibition can improve outcome two mouse models In our azoxymethane/dextran sodium sulfate (AOM/DSS) model...
The antigen-presenting molecule MR1 (MHC class I-related protein 1) presents metabolite antigens derived from microbial vitamin B
Abstract The incidence of HPV-associated (HPV+) head and neck squamous cell carcinoma (HNSCC) has been increasing in recent decades. EGFR inhibitor cetuximab (CTX) is less active HPV+ than HPV- HNSCC. HPV oncoproteins E6 E7 induce HER3 overexpression, EGFR-HER3 heterodimer-driven survival signaling via PIK3, AKT ERK1-2 contributes to resistance the erlotinib. We demonstrate that although panHER inhibitors afatinib dacomitinib more effectively inhibit growth HNSCC cells do erlotinib or...
Highly elevated expression of the oncogene Aurora kinase A (AURKA) occurs in numerous human cancers harboring defective p53, nominating AURKA as a potential vulnerability TP53-mutated cancer. However, clinical trials have indicated modest monotherapy activity inhibitors. Here, we demonstrate that inhibition promotes phosphorylation Replication Protein (RPA), resulting stalled DNA replication fork progression and eliciting stress response multiple models, creating druggable dependence on...
Abstract Single-molecule localization microscopy super-resolution methods rely on stochastic blinking/binding events, which often occur multiple times from each emitter over the course of data acquisition. Typically, events are treated as independent without an attempt to assign them a particular emitter. Here, we describe Bayesian method inferring positions tagged molecules by exploring possible grouping and combination localizations events. The results position estimates that have improved...
Hypercoagulable state is linked to cancer progression; however, the precise role of coagulation cascade poorly described. Herein, we examined contribution a hypercoagulative through administration intravenous Coagulation Factor Xa (FXa), on growth solid human tumors and experimental metastasis B16F10 melanoma in mouse models. FXa increased tumor volume lung, liver, kidney lymph node tail-vein injected cells. Concentrating model, upon coadministration anticoagulant Dalteparin, lung was...
MR1 is a highly conserved microbial immune-detection system in mammals. It captures vitamin B–related metabolite antigens from diverse microbes and presents them at the cell surface to stimulate MR1-restricted lymphocytes including mucosal-associated invariant T (MAIT) cells. presentation MAIT recognition mediate homeostasis through host defense tissue repair. The cellular mechanisms regulating expression are critical its function recognition, yet they poorly defined. Here, we report that...
Dendritic cell (DC) trafficking from peripheral tissues to lymph nodes is a key step required initiate T responses against pathogens as well tumors. In this context, cellular membrane protrusions and the actin cytoskeleton are essential guide DC migration towards chemotactic signals. Caveolin-1 scaffolding protein that modulates signaling pathways leading remodeling of enhanced cancer cells. However, whether caveolin-1 relevant for function specifically unknown. Here we show expression...
Hummingbirds show remarkable adaptation to high altitude hypoxia whereas humans are imperfectly adapted living. Here we compare hemoglobin levels and metabolism derived from growth rhythms in hummingbirds humans. To rhythms, analyzed intervals hummingbird tail feathers human growing tissues such as hair. We find that were higher (P<0.001) than humans, but the influence of on was more pronounced (slope, steeper with increasing altitude, P<0.001), for both taxa converge at extreme...
Abstract Despite gold‐based nanomaterials having a unique role in nanomedicine, among other fields, synthesis limitations relating to reaction scale‐up and control result prohibitively high gold nanoparticle costs. In this work, new preparation procedure for lipid bilayer‐coated nanoparticles water is presented, using sodium oleate as reductant capping agent. The seed‐free not only allows size precision (8–30 nm) but also remarkable particle concentration (10 m Au). These efficiencies allow...
Gold Nanoparticles In article number 2309919, Julie B. Zimmerman and co-workers present a new multiplexable synthesis of gold nanoparticles in an aqueous system using sodium oleate as reductant coating agent. Using this approach, arrays size-controlled (8–30 nm) nanoparticles, up to 10 mM Au concentration, were synthesized 96-well plates thermal cycler. Synthesis scale-up 0.5 g was demonstrated.
Abstract Human papillomavirus (HPV)-negative head and neck squamous cell carcinoma (HNSCC) lung cancer mainly harbor disruptive mutations in TP53 and/or CDKN2A tumor suppressor genes, which are associated with refractoriness to treatment as well poor prognosis survival. Mutational loss of function fosters elevated expression Aurora kinase A (AURKA) that plays a crucial role mitotic progression at G2/M stabilizes DNA replication forks S phase. We have previously demonstrated combined...