A5068698824- Advanced Electron Microscopy Techniques and Applications
- RNA and protein synthesis mechanisms
- Electron and X-Ray Spectroscopy Techniques
- RNA modifications and cancer
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Virus-based gene therapy research
- RNA Research and Splicing
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Force Microscopy Techniques and Applications
- Bacteriophages and microbial interactions
- Alzheimer's disease research and treatments
- Viral Infections and Immunology Research
- Integrated Circuits and Semiconductor Failure Analysis
- Peptidase Inhibition and Analysis
- Endoplasmic Reticulum Stress and Disease
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Microtubule and mitosis dynamics
- Protein Structure and Dynamics
- Molecular Sensors and Ion Detection
Florida State University
2016-2025
The University of Queensland
2020
Georgia Institute of Technology
2003-2018
Scripps Research Institute
2005-2012
Torrey Pines Institute For Molecular Studies
2006
Health & Safety Laboratory
2005
University of Alabama at Birmingham
2000-2003
University of California, Davis
2001
Cancer Research Center
2001
University of Warwick
1997
The chaperonin GroEL assists the folding of nascent or stress-denatured polypeptides by actions binding and encapsulation. ATP initiates a series conformational changes triggering association cochaperonin GroES, followed further large movements that eject substrate polypeptide from hydrophobic sites into GroES-capped, hydrophilic chamber. We used cryo-electron microscopy, statistical analysis, flexible fitting to resolve set distinct GroEL-ATP conformations can be ordered trajectory domain...
Bacterial and archaeal clustered regularly interspaced short palindromic repeat (CRISPR) loci capture virus plasmid sequences use them to recognize eliminate these invaders. CRISPR RNAs (crRNAs) containing the acquired are incorporated into effector complexes that destroy matching invader nucleic acids. The multicomponent Cmr complex cleaves RNA targets complementary crRNAs. Here, we report cryoelectron microscopy reconstruction of a functional bound with target at ∼12 Å. Pairs Cmr4 Cmr5...
In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for deposition and validation of cryoEM structures, with focus on single-particle analysis. The meeting attended by 45 experts in processing, model building refinement, validation, archiving such structures. This report describes the workshop's motivation history, topics discussed, consensus recommendations resulting from workshop. Some challenges future methods-development efforts this area are also...
Adeno-associated virus (AAV) vectors are preeminent in emerging clinical gene therapies. Generalizing beyond the most tractable genetic diseases will require modulation of cell specificity and immune neutralization. Interactions AAV with its cellular receptor, AAVR, key to understanding cell-entry trafficking rigor needed engineer tissue-specific vectors. Cryo-electron tomography shows ordered binding part flexible receptor viral surface, distal domains multiple conformations. Regions close...
Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell between organelles. Proteins included clathrin-coated (CCVs) category include clathrin heavy chain (CHC), light (CLC), a variety adaptor protein complexes. Much is known about structures individual CCV components, but data are lacking fully assembled complexes together with membrane complex cargo. Here, we determined natively CCVs geometries. We show that β2 appendages crosslink adjacent CHC β-propellers...
The Cmr complex is the multisubunit effector of type III-B clustered regularly interspaced short palindromic repeats (CRISPR)-Cas immune system. recognizes a target RNA through base pairing with integral CRISPR (crRNA) and cleaves at multiple spaced locations within complementary region. To understand molecular basis function this complex, we have assembled information from electron microscopic X-ray crystallographic structural studies mutagenesis complete Pyrococcus furiosus complex. Our...
Bacterial ribosome rescue pathways that remove ribosomes stalled on mRNAs during translation have been proposed as novel antibiotic targets because they are essential in bacteria and not conserved humans. We previously reported the discovery of a family acylaminooxadiazoles selectively inhibit trans-translation, main pathway bacteria. Here, we report optimization pharmacokinetic properties acylaminooxadiazoles, producing MBX-4132, which clears multiple-drug resistant Neisseria gonorrhoeae...