A5043733417- Health Systems, Economic Evaluations, Quality of Life
- Cancer Genomics and Diagnostics
- Genomics and Rare Diseases
- Economic and Financial Impacts of Cancer
- BRCA gene mutations in cancer
- Economic and Environmental Valuation
- Genetic factors in colorectal cancer
- Pharmaceutical Economics and Policy
- Patient-Provider Communication in Healthcare
- Healthcare Policy and Management
- Plant tissue culture and regeneration
- Ethics in Clinical Research
- Biomedical Ethics and Regulation
- CAR-T cell therapy research
- Statistical Methods in Clinical Trials
- Innovation Policy and R&D
- Cytomegalovirus and herpesvirus research
- Palliative Care and End-of-Life Issues
- Lymphoma Diagnosis and Treatment
- Genomic variations and chromosomal abnormalities
- Autoimmune and Inflammatory Disorders Research
- Adolescent and Pediatric Healthcare
- Plant-Microbe Interactions and Immunity
- Childhood Cancer Survivors' Quality of Life
- HIV Research and Treatment
University of British Columbia
2016-2025
Canadian Centre for Applied Research in Cancer Control
2014-2024
Cancer Research Institute
2024
BC Cancer Agency
2013-2023
McGill University
2023
Health Net
2023
University Health Network
2023
Cancer Research UK
2021
Spinal Cord Injury BC
2020
Okanagan University College
2020
Recent advances are enabling delivery of precision genomic medicine to cancer clinics. While the majority approaches profile panels selected genes or hotspot regions, comprehensive data provided by whole-genome and transcriptome sequencing analysis (WGTA) present an opportunity align a much larger proportion patients therapies.Samples from 570 with advanced metastatic diverse types enrolled in Personalized OncoGenomics (POG) program underwent WGTA. DNA-based data, including mutations, copy...
The complete nucleotide sequence of an infectious clone simian immunodeficiency virus macaques, SIVmac239, has been determined. Virus produced from this molecular causes AIDS in rhesus monkeys a time frame suitable for laboratory investigation. proviral genome including both long terminal repeats is 10,279 base pairs length and contains open reading frames gag, pol, vif, vpr, vpx, tat, rev, env. nef gene in-frame premature stop after the 92nd codon. At level, SIVmac239 closely related to...
The so-called "nonessential" genes of primate lentiviruses can be deleted without abrogating the ability virus to replicate under at least some cell culture conditions. In SIVmac, these are vif, vpx, vpr, and nef. Sequences in upstream region U3 LTR have also been shown dispensable for replication culture. We report here construction characterization a panel 40 single combination deletion mutants derived from pathogenic molecular clone SIVmac239. Deletion vpr gene caused little or no change...
The virulence genes of the Agrobacterium tumefaciens Ti plasmid are grouped into six transcription units and direct transfer T-DNA plant cells.We report here nucleotide sequence largest vir operon, virB, from pTiA6NC.This operon contains 11 open reading frames, 7 which show evidence translational coupling.trpE:virB gene fusions were used to confirm frames virB2, 4, 5 , 6, 7, 8, 10, 11.In addition, native products virB6 virB9 identified using maxicell in vitro transcriptiontranslation...
The production of cytokinins by plant-associated bacteria was examined radioimmunoassay. Strains producing trans-zeatin were identified in the genera Agrobacterium and Pseudomonas. tumefaciens strains containing nopaline tumor-inducing plasmids, A. Lippia isolates, rhizogenes produced culture at 0.5 to 44 micrograms/liter. Pseudomonas solanacearum syringae pv. savastanoi levels up 1 mg/liter. In vitro cytokinin biosynthetic activity measured for representative found correlate with...
<h3>Background:</h3> An important challenge with the application of next-generation sequencing technology is possibility uncovering incidental genomic findings. A paucity evidence on personal utility for findings has hindered clinical guidelines. Our objective was to estimate complex information derived from <h3>Methods:</h3> We used a discrete-choice experiment evaluate participants’ following attributes: disease penetrance, treatability, severity, carrier status and cost. Study...
<h3>Background:</h3> The choice between palliative chemotherapy (defined as the use of cytotoxic medications delivered intravenously for purpose our study) and supportive care alone is one most difficult decisions in pediatric oncology, yet little known about preferences parents health professionals. We compared strength these by considering children's quality life survival time key attributes. In addition, we identified factors associated with reported preferences. <h3>Methods:</h3>...
Abstract The purpose of this study was to develop a brief instrument, the Feelings About genomiC Testing Results (FACToR), measure psychosocial impact returning genomic findings patients in research and clinical practice. To create FACToR, we modified augmented Multidimensional Impact Cancer Risk Assessment (MICRA) questionnaire based on from literature review, two focus groups ( N = 12), cognitive interviews 6). We evaluated data 122 participants referred for evaluation inherited colorectal...
Purpose: To determine real-world diagnostic rates, cost trajectories, and cost-effectiveness of exome sequencing (ES) genome (GS) for children with developmental and/or seizure disorders in British Columbia, Canada.Methods: Based on medical records review, we estimated costs outcomes 491 patients who underwent standard care (SOC) testing at BC Children's Hospital.Results informed a state-transition Markov model examining three competing strategies: (1) SOC last-tier access to ES; (2)...
Auxiliary genes that are not essential for viral replication in cell culture found all known lentiviruses. The "nonessential" auxiliary of HIV-1 vif, vpr, vpu, and nef. Sequences within the upstream region U3 LTR also required virus culture. We constructed a panel 23 mutants with single combination deletions these regions wild-type infectious molecular clone NL4-3. Deletion nef sequence (US), individually or combinations, did appreciably alter either chimpanzee PBMCs, human B/T hybrid line...
The so-called "nonessential" genes of primate lentiviruses can be deleted without abrogating the ability virus to replicate under at least some cell culture conditions. In SIVmac, these are vif, vpx, vpr, and nef. Sequences in upstream region U3 LTR have also been shown dispensable for replication culture. We report here construction characterization a panel 40 single combination deletion mutants derived from pathogenic molecular clone SIVmac239. Deletion vpr gene caused little or no change...
Several loci on the tumor-inducing plasmid from Agrobacterium tumefaciens were transcriptionally activated in presence of wounded plant tissue or extracts. The inducible virulence required for efficient tumor formation. In contrast, plant-inducible locus pinF was not observed to be absolutely essential virulence. Mutants showed an attenuated a variety dicotyledonous hosts, and this attenuation became more pronounced with decreasing numbers bacterial cells inoculum. DNA sequence 5.5-kilobase...
Abstract Background Limited data exist on the real‐world costs of applying whole‐genome analysis ( WGA ) in a clinical setting. We estimated to guide treatments for patients with advanced cancers and characterized how evolve over time. Methods The setting is British Columbia Cancer Agency Personalized OncoGenomics POG program Columbia, Canada. Cost were obtained who enrolled from 2012 2015. mean using bootstrapping. applied time series produced 10‐year forecasts determine when are expected...