A5068952473- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Microbial Natural Products and Biosynthesis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Pluripotent Stem Cells Research
- Hydrogen Storage and Materials
- Renal and related cancers
- Antibiotic Resistance in Bacteria
- Radar Systems and Signal Processing
- Nuclear Materials and Properties
- Advanced SAR Imaging Techniques
- Genomics and Phylogenetic Studies
- Ammonia Synthesis and Nitrogen Reduction
- Xenotransplantation and immune response
- Sperm and Testicular Function
- Music and Audio Processing
- Seed Germination and Physiology
- Virus-based gene therapy research
- Seaweed-derived Bioactive Compounds
- Epigenetics and DNA Methylation
- Fatigue and fracture mechanics
- Marine Sponges and Natural Products
- Chemical Synthesis and Analysis
Johns Hopkins University
2011-2025
Nanjing Medical University
2015-2024
Chinese Academy of Sciences
2009-2024
Qingdao National Laboratory for Marine Science and Technology
2024
Institute of Oceanology
2012-2024
Hangzhou Institute of Applied Acoustics
2023
South China Institute of Collaborative Innovation
2022-2023
Beijing University of Posts and Telecommunications
2016-2023
Computer Network Information Center
2023
Guizhou University
2022
As nitric oxide (NO) plays significant roles in a variety of physiological processes, the capability for real-time and accurate detection NO live organisms is great demand. Traditional assessments rely on indirect colorimetric techniques or electrochemical sensors that often comprise rigid constituent materials can hardly satisfy sensitivity spatial resolution simultaneously. Here, we report flexible highly sensitive biosensor based organic transistors (OECTs) capable continuous wireless...
Abstract Neuromorphic electronics has demonstrated great promise in mimicking the sensory and memory functions of biological systems. However, synaptic devices with desirable sensitivity, selectivity, operational voltage imitating olfactory system have rarely been reported. Here, a flexible biomimetic synapse based on an organic electrochemical transistor (OECT) coupled breath‐figure derived porous solid polymer electrolyte (SPE) is proposed. The device demonstrates excellent sensitivity...
Abstract Efficient isolation of embryonic stem (ES) cells from pre-implantation porcine embryos has remained a challenge. Here, we describe the derivation stem-like (pESLCs) by seeding isolated inner cell mass (ICM) in vitro -produced blastocyst into α-MEM with basic fibroblast growth factor (bFGF). The pESL kept normal karyotype and displayed flatten clones, similar phenotype to human (hES cells) rodent epiblast cells. These exhibited alkaline phosphatase (AP) activity expressed...
The principal cause of bacterial resistance to penicillin and other β-lactam antibiotics is the acquisition plasmid-encoded β-lactamases, enzymes that catalyze hydrolysis bond render these inactive. Clavulanic acid a potent inhibitor β-lactamases has proven clinically effective in combating resistant infections. Although clavulanic share marked structural similarities, biosyntheses their bicyclic nuclei are wholly dissimilar. In contrast efficient iron-mediated oxidative cyclization...
The 26S proteasome, which is a multi-subunit protease with specificity for substrate proteins that are postranslationally modified by ubiquitination, has been implicated in acrosomal function and sperm-zona pellucida (ZP) penetration during mammalian fertilization. Ubiquitin C-terminal hydrolases (UCHs) responsible the removal of polyubiquitin chains priming proteasomal proteolysis. inhibition deubiquitination increases rate Consequently, we have hypothesized sperm acrosome-borne UCHs...
Nuclear transfer efficiency in the pig is low and thought to be caused by inadequate nuclear reprogramming. The objective of this study was identify differentially represented transcripts vivo derived (BLIVV), vitro fertilized (BLIVF), or (NT, three different activation methods) blastocyst stage embryos donor cell line microarray analysis, determine if treatment reconstructed with histone deacetylase inhibitor, Scriptaid (NTS), for 14 h postactivation would correct gene expression a subset...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTOrigin of the β-Lactam Carbons in Clavulanic Acid from an Unusual Thiamine Pyrophosphate-Mediated ReactionNusrat Khaleeli, Rongfeng Li, and Craig A. TownsendView Author Information Department Chemistry, The Johns Hopkins University Baltimore, Maryland 21218 Cite this: J. Am. Chem. Soc. 1999, 121, 39, 9223–9224Publication Date (Web):September 16, 1999Publication History Received6 July 1999Published online16 September inissue 1 October...
It has been notoriously difficult to successfully cryopreserve swine embryos, a task that even more for in vitro-produced embryos. The first reproducible method of cryopreserving vivo-produced embryos was after centrifugation and removal the lipids. Here we report adaptation similar process permits cryopreservation somatic cell nuclear transfer (SCNT) These develop blastocyst stage survive cryopreservation. Transfer 163 cryopreserved SCNT two surrogates produced 10 piglets. Application this...
Abstract The following experiments compared the efficiency of three fusion/activation protocols somatic cell nuclear transfer (SCNT) with porcine cells transfected enhanced green fluorescent protein driven by chicken β‐actin/rabbit β‐globin hybrid promoter (pCAGG‐EGFP). included electrical (NT1), followed treatment a reversible proteasomal inhibitor MG132 (NT2) and fusion in low Ca 2+ chemical activation thimerosal/dithiothreitol (NT3). Data were collected at Days 6, 12, 14, 30, 114...
ABSTRACT Miniature pigs have advantages over rodents in modeling atherosclerosis because their cardiovascular system and physiology are similar to that of humans. Apolipoprotein E (ApoE) deficiency has long been implicated disease To establish an improved large animal model familial hypercholesterolemia atherosclerosis, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) was used disrupt ApoE gene Bama miniature pigs. Biallelic-modified...
Myostatin (Mstn) is an inhibitor of myogenesis, regulating the number and size skeletal myocytes. In addition to its myogenic regulatory function, Mstn plays important roles in development adipose tissues metabolism. present study, knockout rat model was generated using zinc finger nuclease (ZFN) technique order further investigate function mechanism The possesses a frame shift mutation resulting early termination codon truncated peptide 109 amino acids rather than full 376 acids. absence...
(5R)-Carbapen-2-em-3-carboxylic acid is the simplest structurally among naturally occurring carbapenem beta-lactam antibiotics. It co-occurs with two saturated (3S,5S)- and (3S,5R)-carbapenam carboxylic acids. Confusion persists in literature about signs of rotation absolute configurations these compounds that resolved this paper. (3S,5S)-Carbapenam was prepared from L-pyroglutamic to unambiguously establish its configuration as identical natural product isolated Serratia marcescens...