A5030729464- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- Virus-based gene therapy research
- Membrane Separation Technologies
- Bone and Dental Protein Studies
- Biomedical Research and Pathophysiology
- Biopolymer Synthesis and Applications
- Phytochemical and Pharmacological Studies
- Nanopore and Nanochannel Transport Studies
- Oral microbiology and periodontitis research
- Immune Response and Inflammation
- Barrier Structure and Function Studies
- Retinal Diseases and Treatments
- Phytochemistry and Biological Activities
- Glaucoma and retinal disorders
- Monoclonal and Polyclonal Antibodies Research
- Histone Deacetylase Inhibitors Research
- Phytochemicals and Antioxidant Activities
Hoshi University
2020-2024
Ebara (Japan)
2022-2024
Shanghai First People's Hospital
2018-2021
Shanghai Jiao Tong University
2021
Nanjing Medical University
2018
Coconut Research Institute
2012
Chinese Academy of Tropical Agricultural Sciences
2012
Formulation of cationic liposomes is a key factor that determine the gene knockdown efficiency by liposomes/siRNA complexes (siRNA lipoplexes). Here, to optimal combination lipid and phospholipid in for vitro vivo using siRNA lipoplexes, three types were used, namely 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), dimethyldioctadecylammonium bromide (DDAB) 11-[(1,3-bis(dodecanoyloxy)-2-((dodecanoyloxy)methyl)propan-2-yl)amino]-N,N,N-trimethyl-11-oxoundecan-1-aminium (TC-1-12). Thereafter,...
Messenger RNA (mRNA)-based therapies are a novel class of therapeutics used in vaccination and protein replacement for monogenic diseases. Previously, we developed modified ethanol injection (MEI) method small interfering (siRNA) transfection, which cationic liposome/siRNA complexes (siRNA lipoplexes) were prepared by mixing lipid-ethanol solution with siRNA solution. In this study, applied the MEI to prepare mRNA lipoplexes evaluated vitro vivo expression efficiencies. We selected six...
In this paper, a method incorporating ultrasound technology has been developed to extract the phenolic compounds from areca husk. The experiments were carried out according three-variable, five-level central composite rotatable design, and parameters of ethanol, extraction temperature time under optimum ultrasound-mediated condition optimized by response surface methodology. conditions as follows: ethanol concentration, 41%; temperature, 53C; time, 38 min. concentration was most significant...
The expression of secreted protein acidic and rich in cysteine (SPARC) has been recently identified to be associated with the pathology diabetic retinopathy. Therefore, present study aimed evaluate regulatory role SPARC human retinal capillary endothelial cells (HRCECs), following exposure a high glucose environment vitro. cell viability, migration, angiogenesis, permeability levels HRCECs were measured treatment different concentrations (25, 50 or 100 mM). Lentiviral vectors...
In this study, we prepared small interfering RNA (siRNA)/cationic liposome complexes (lipoplexes) modified with folate (FA)-polyethylene glycol (PEG, MW 2000, 3400 or 5000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) to facilitate their uptake into tumor cells via receptor (FR), and PEG1600-cholesterol (PEG1600-Chol) PEG2000-chondroitin sulfate conjugate (PEG2000-CS), enhance systemic stability. Among the FA-PEG-modified siRNA lipoplexes, 0.5 mol% FA-PEG5000-DSPE-modified...
Previously, using three types of cationic lipids, the effect phospholipids in liposomal formulations on gene-knockdown efficacy was determined after vitro and vivo transfection with small interfering RNA (siRNA)/cationic liposome complexes (siRNA lipoplexes) containing various lipids phospholipids. In present study, six other namely N,N-dimethyl-N-tetradecyltetradecan-1-aminium bromide, N-hexadecyl-N,N-dimethylhexadecan-1-aminium bromide (DC-1-16),...
Our previous study reported that reverse (Rev)‑transfection with small interfering RNA (siRNA)/cationic liposome complexes (siRNA lipoplexes) freeze‑dried in trehalose or sucrose solution resulted high gene‑silencing activity cells. The current investigated whether pre‑freezing saccharide types present during the freeze‑drying of siRNA lipoplexes affected cells after Rev‑transfection. Three cationic cholesterol derivatives and three dialkyl trialkyl lipids were used for preparation...
Recently, small interfering RNA (siRNA)/cationic liposome complexes (siRNA lipoplexes) have become a crucial research tool for studying gene function. Easy and reliable siRNA transfection with large set of siRNAs is required the successful screening Reverse (Rev)‑transfection freeze‑dried lipoplexes validated in multi‑well plate. In our previous study, it was shown that Rev‑transfection disaccharides or trisaccharides resulted long‑term stability high level gene‑knockdown activity. present...
Previously, we demonstrated that cationic liposomes comprised of
We have previously demonstrated that messenger RNA (mRNA) lipoplexes composed of
Secreted protein acidic and rich in cysteine (SPARC) may play a notable role aqueous humor outflow through the trabecular meshwork (TM). SPARC is potential therapeutic target glaucoma, mechanism by which it regulates intraocular pressure remains unclear. The present study aimed to observe effects of human TM cells (HTMCs) <em>in vitro</em>. was downregulated recombinant lentiviral vectors HTMCs, subsequent levels F‑actin expression, zonula occludens‑1 (ZO‑1) expression cellular phagocytosis...