A5066770861- Bacterial Genetics and Biotechnology
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Botanical Research and Chemistry
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Signaling Pathways in Disease
- Ion Channels and Receptors
- Influenza Virus Research Studies
- Advanced Biosensing Techniques and Applications
Case Western Reserve University
2023-2025
University School
2023-2024
Calcium signaling is a fundamental molecular means of cellular regulation. Store operated calcium entry (SOCE) major intracellular module, wherein release from the endoplasmic reticulum triggers transmembrane STIM1 proteins to conformationally shift and oligomerize prompt influx extracellular environment. senses ER concentrations with its canonical EF-hand domain, missense variants can dysregulate SOCE cause Tubular Aggregate Myopathy, Stormorken Syndrome or immunodeficiency. Few are...
Typical mammalian overexpression systems test protein sequence variants with little control over expression levels and steady-state abundances, hindering interpretations of how converge to yield phenotypic outcomes. We explored the translation initiation sequence, commonly referred as Kozak a means modulate abundance cellular function. performed sort-seq on randomized library 6 nucleotides preceding start codon, amounting 4,042 sequences. Calibrating scores revealed ~100-fold range...
The Bxb1 bacteriophage serine DNA recombinase is an efficient tool for engineering recombinant into the genomes of cultured cells. Generally, a single engineered "landing pad" site introduced cell genome, permitting integration transgenic circuits or libraries transgene variants. While sufficient many studies, extent genetic manipulation possible with limiting and insufficient more complex cell-based assays. Here, we harnessed two orthogonal sites to enable alternative avenues using...