A5044020651- Adhesion, Friction, and Surface Interactions
- Tribology and Lubrication Engineering
- Iterative Learning Control Systems
- Lysosomal Storage Disorders Research
- Metal Forming Simulation Techniques
- Engineering Applied Research
- Molecular Sensors and Ion Detection
- Laser and Thermal Forming Techniques
- Structural Load-Bearing Analysis
- Bone Metabolism and Diseases
- Trypanosoma species research and implications
- Advanced Surface Polishing Techniques
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Bone health and treatments
- Structural Response to Dynamic Loads
- Advancements in Photolithography Techniques
- Geotechnical Engineering and Underground Structures
- Metal Alloys Wear and Properties
- Fatigue and fracture mechanics
- Parvovirus B19 Infection Studies
- Advanced optical system design
- Analytical Chemistry and Sensors
- Gastroesophageal reflux and treatments
- Wnt/β-catenin signaling in development and cancer
Comprehensive Cancer Center Vienna
2025
Medical University of Vienna
2020-2025
Nagasaki Institute of Applied Science
2004-2024
Spanish National Cancer Research Centre
2018
Centro de Investigación del Cáncer
2018
National Center for Geriatrics and Gerontology
2013-2018
Tokushima University
2006-2011
National Institute of Biomedical Innovation, Health and Nutrition
2011
Japan Science and Technology Agency
2006-2010
Nippon Medical School
1990-2006
Bone remodeling is characterized by the sequential, local tethering of osteoclasts and osteoblasts key to maintenance bone integrity. While matrix-mobilized growth factors, such as TGF-β, are proposed regulate remodeling, no in vivo evidence exists that an osteoclast-produced molecule serves a coupling factor for resorption formation. We found CTHRC1, protein secreted mature bone-resorbing osteoclasts, targets stromal cells stimulate osteogenesis. Cthrc1 expression was robustly induced when...
ABSTRACT Bone remodeling is regulated by a coupling of resorption to subsequent formation; however, the “coupling factor” and underlying mechanism are not fully understood. Here, we found that condition medium (CM) mature osteoclasts contains humoral factor stimulates differentiation primary osteoblasts, as determined alkaline phosphatase (ALP) activity. We purified osteoblastogenesis-stimulating activity from 3 L osteoclast CM through successive ion exchange chromatographies monitoring ALP...
Abstract Osteosarcoma (OS) is the most frequent primary malignant bone tumor in urgent need of better therapies. Using genetically modified mouse models (GEMMs), we demonstrate that Wnt signaling promotes c-Fos-induced OS formation via actions collagen-modifying enzyme Loxl2. c-Fos/AP-1 directly regulates expression ligands Wnt7b and Wnt9a cells through promoter binding, turn promote Loxl2 murine human transcription factors Zeb1 Zeb2. Concordantly, inhibition ligand secretion by inactivating...
The activator protein-1 (AP-1) transcription factor component c-Fos regulates chondrocyte proliferation and differentiation, but its involvement in osteoarthritis (OA) has not been functionally assessed.c-Fos expression was evaluated by immunohistochemistry on articular cartilage sections from patients with OA mice subjected to the destabilisation of medial meniscus (DMM) model OA. Cartilage-specific knockout (c-FosΔCh) were generated crossing c-fosfl/fl Col2a1-CreERT mice. Articular...
Genetically engineered mouse models (GEMMs) are instrumental for modelling local and systemic features of complex diseases such as cancer. Non-invasive, longitudinal cell detection monitoring in tumors, metastases and/or the micro-environment is paramount to achieve a better spatiotemporal understanding cancer progression evaluate therapies preclinical studies. Bioluminescent fluorescent reporters marking tumor cells or their microenvironment valuable non-invasive vivo. Here we report...
Abstract Objective: Novel recombinant human lysosomal β‐hexosaminidase A (HexA) was developed for enzyme replacement therapy (ERT) Tay‐Sachs and Sandhoff diseases, ie, autosomal recessive GM2 gangliosidoses, caused by HexA deficiency. Methods: ( Om4 HexA) with a high mannose 6‐phosphate (M6P)‐type‐N‐glycan content, which produced methylotrophic yeast strain, Ogataea minuta , overexpressing the OmMNN4 gene, intracerebroventricularly (ICV) administered to disease model mice Hexb −/− mice) at...
To develop a novel enzyme replacement therapy for neurodegenerative Tay-Sachs disease (TSD) and Sandhoff (SD), which are caused by deficiency of β-hexosaminidase (Hex) A, we designed genetically engineered HEXB encoding the chimeric human β-subunit containing partial amino acid sequence α-subunit structure-based homology modeling. We succeeded in producing modified HexB Chinese hamster ovary (CHO) cell line stably expressing HEXB, can degrade artificial anionic substrates GM2 ganglioside...
To find a new biomarker of Tay-Sachs disease and Sandhoff disease. The lyso-GM2 ganglioside (lyso-GM2) levels in the brain plasma mice were measured by means high performance liquid chromatography effect modified hexosaminidase (Hex) B exhibiting Hex A-like activity was examined. Then, concentrations human samples determined. apparently increased compared with those wild-type mice, they decreased on intracerebroventricular administration B. patients increased, increase associated decrease A...
Osteosarcoma (OS) is a rare tumor of the bone occurring mainly in young adults accounting for 5% all childhood cancers. Because limited therapeutic options, there has been no survival improvement OS patients past 40 years. The epidermal growth factor receptor (EGFR) highly expressed OS; however, its clinical relevance unclear. Here, we employed an autochthonous c-Fos-dependent mouse model (H2-c-fosLTR) and human biopsies preclinical studies aimed at identifying novel biomarkers benefits...
ABSTRACT The osteoclast-derived collagen triple helix repeat containing 1 (CTHRC1) protein stimulates osteoblast differentiation, but the underlying mechanism remains unclear. Here, we identified Wnt-activated inhibitory factor (WAIF1)/5T4 as a cell-surface binding CTHRC1. WAIF1-encoding Trophoblast glycoprotein (Tpbg) gene, which is abundantly expressed in brain and bone not other tissues, showed same expression pattern Cthrc1. Tpbg downregulation marrow stromal cells reduced CTHRC1...
Hard disk drives (HDD) are improving rapidly in recording density and transfer rate every year. A significant factor contributing to their evolution is the spindle motor. Development of a motor using fluid dynamic bearing (FDB) desirable as conventional motors incorporating ball bearings expected reach limitation technically near future. In this paper we discuss trends HDD, requirements for HDD motors, construction, problems relation magnetic circuits, constituent FDB technologies.
A new dual-stage actuator (DSA) for positioning a magnetic head high-density hard disk drives was proposed and developed. This DSA device is characterized by applying sputter-deposited thin-film lead-zirconate-titanate material (PZT), instead of the previously bulk type actuator. The performance found to be outstanding with peak-to-peak displacement 1.2 /spl mu/m at an applied voltage plusmn/5 V, suspension resonance frequency higher than 15 kHz, yielding servo bandwidth more several kHz.
Human lysosomal beta-hexosaminidase A is a heterodimer composed of alpha- and beta-subunits encoded by HEXA HEXB, respectively. We genetically introduced an additional N-glycosylation sequon into HEXA, which caused amino acid substitutions (S51 to N A53 T) at homologous positions N84 T86 in the beta-subunit. The mutant HexA (NgHexA) obtained from Chinese hamster ovary (CHO) cell line co-expressing mutated wild-type HEXB complementary DNAs was demonstrated contain mannose-6-phosphate...
A new dual-stage actuator for positioning a magnetic head high-density hard disk drives was proposed and developed. This device is characterized by the sputter-deposited thin-film PZT, featuring large displacement of 1.2 /spl mu/m high-resonance frequency over 15 kHz. In this paper, reliability against temperature, humidity, mechanical shock investigated to ensure practical application drive products.
We randomly administered cephalosporins or macrolides to 365 pediatric patients with influenza-like symptoms and compared the clinical course complication rate of pneumonia. One hundred fifty-four received (Group 1) 211 2). There were no significant differences in age, male/female ratio body weight between two groups. Macrolides alleviated fever significantly faster than cephalospoins (3.8± 1.4 days vs 4.3± days), though maximum temperature showed difference Thirty-nine underwent laboratory...
In this paper, we propose a novel shockproof head gimbals assembly (HGA) structure incorporated with "a balanced-type suspension" for 0.85-in hard disk drives (HDDs), and investigated its performance on external shock resistance during read/write operations. Experimental drop impact test results confirm that the drive equipped new suspension is durable over 2000 G (1.0 ms duration) of shock, ensuring no slider and/or surface damage. According to computer simulation experimental results,...
Abstract Background: Local force distribution supporting the bodyweight of infants with Down syndrome (DS) appears to be different from that healthy controls. The purpose present study was establish methods assess this and allow therapeutic evaluation neurological development in DS prior walking. Methods: Contact pressure patterns supine prone positions were measured by photoelastic compared between group included eight subjects, seven regular trisomy 21, one a Robertson translocation....
An automatic design method for all types of brushless DC motors VCRs was developed. Quick and easy motor with no trial manufacture realized, enabling a minimum size to achieve maximum efficiency. In carrying out this work, the authors systematized parameters governing characteristics theoretically analyzed clarified correlations between characteristics. Various experiments were conducted increase reliability theoretical analysis magnetic circuit. Some rules vital in identified used devise an...