A5048458683- Amyotrophic Lateral Sclerosis Research
- RNA Research and Splicing
- Neurogenetic and Muscular Disorders Research
- Bioactive Compounds and Antitumor Agents
- Prion Diseases and Protein Misfolding
- Psidium guajava Extracts and Applications
- Morinda citrifolia extract uses
- Parkinson's Disease Mechanisms and Treatments
- Nuclear Structure and Function
- Plant Pathogenic Bacteria Studies
- Insect symbiosis and bacterial influences
- Genetic Neurodegenerative Diseases
- Bacterial biofilms and quorum sensing
- Essential Oils and Antimicrobial Activity
- Aluminum toxicity and tolerance in plants and animals
- RNA modifications and cancer
- Chemistry and Chemical Engineering
- Legume Nitrogen Fixing Symbiosis
- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Cellular Mechanics and Interactions
- RNA regulation and disease
University of Zurich
2018-2024
Quantitative BioSciences
2020-2024
Association pour la Recherche de Thérapeutiques Innovantes en Cancérologie
2022
Max Planck Institute of Biochemistry
2018-2021
Abstract Aggregation of the RNA‐binding protein TAR DNA‐binding 43 (TDP‐43) is key neuropathological feature neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). In physiological conditions, TDP‐43 predominantly nuclear, forms oligomers, contained in biomolecular condensates assembled by liquid–liquid phase separation (LLPS). disease, cytoplasmic or intranuclear inclusions. How transitions from to pathological states remains...
Human cellular models of neurodegeneration require reproducibility and longevity, which is necessary for simulating age-dependent diseases. Such systems are particularly needed TDP-43 proteinopathies
Mutations disrupting the nuclear localization of RNA-binding protein FUS characterize a subset amyotrophic lateral sclerosis patients (ALS-FUS). regulates RNAs, but its role at synapse is poorly understood. Using super-resolution imaging we determined that within synapses occurs predominantly near vesicle reserve pool presynaptic sites. CLIP-seq on synaptoneurosomes, identified synaptic RNA targets, encoding proteins associated with organization and plasticity. Significant increase during...
The global rise of antibiotic resistance calls for new drugs against bacterial pathogens. A common approach is to search natural compounds deployed by microbes inhibit competitors. Here, we show that the iron-chelating pyoverdines, siderophores produced environmental Pseudomonas spp., have strong antibacterial properties inducing iron starvation and growth arrest in screen 320 isolates used 12 human pathogens uncovered several pyoverdines with particularly high distinct chemical...
The most frequent genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia is a G 4 C 2 repeat expansion in the C9orf72 gene. This gives rise to translation aggregating dipeptide (DPR) proteins, including poly-GA as abundant species. However, gain toxic function effects have been attributed either DPRs or pathological RNA. Here, we analyzed cellular model relative toxicity Cytoplasmic aggregates, generated absence RNA, interfered with nucleocytoplasmic protein transport,...
Article22 November 2021Open Access Transparent process FTLD-TDP assemblies seed neoaggregates with subtype-specific features via a prion-like cascade Pierre De Rossi orcid.org/0000-0002-2356-4269 Department of Quantitative Biomedicine, University Zurich, Switzerland Search for more papers by this author Amanda J Lewis orcid.org/0000-0001-7257-5848 Laboratory Biological Electron Microscopy, Institute Physics, SB, EPFL, Fundamental Microbiology, Faculty Biology and Medicine, UNIL, Lausanne,...
The global rise of antibiotic resistance calls for new drugs against bacterial pathogens. A common approach is to search natural compounds deployed by microbes inhibit competitors. Here we show that the iron chelating pyoverdines, siderophores produced environmental Pseudomonas spp., have strong antibacterial properties inducing starvation and growth arrest in screen 320 isolates used 12 human pathogens uncovered several pyoverdines with particularly high distinct chemical characteristics....
Hexanucleotide G 4 C 2 repeat expansions in the C9orf72 gene are most common genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia. Dipeptide proteins (DPRs) generated by translation repeat-containing RNAs show toxic effects vivo as well vitro key targets for therapeutic intervention. We human antibodies that bind DPRs with high affinity specificity. Anti-GA engaged extra- intra-cellular poly-GA reduced aggregate formation a overexpressing cell line. However, antibody...
Abstract Aggregation of the RNA-binding protein TDP-43 is main common neuropathological feature proteinopathies. In physiological conditions, predominantly nuclear and contained in biomolecular condensates formed via liquid-liquid phase separation (LLPS). However, disease, depleted from these compartments forms cytoplasmic or, sometimes, intranuclear inclusions. How transitions to pathological states remains poorly understood. Here, we show that self-oligomerization RNA binding cooperatively...
Abstract The global rise of antibiotic resistance calls for new drugs against bacterial pathogens. A common approach is to search natural compounds deployed by microbes inhibit competitors. Here we show that the iron chelating pyoverdines, siderophores produced environmental Pseudomonas spp., have strong antibacterial properties inducing starvation and growth arrest in screen 320 isolates used 12 human pathogens uncovered several pyoverdines with particularly high distinct chemical...
The global rise of antibiotic resistance calls for new drugs against bacterial pathogens. A common approach is to search natural compounds deployed by microbes inhibit competitors. Here we show that the iron chelating pyoverdines, siderophores produced environmental Pseudomonas spp., have strong antibacterial properties inducing starvation and growth arrest in screen 320 isolates used 12 human pathogens uncovered several pyoverdines with particularly high distinct chemical characteristics....
The global rise of antibiotic resistance calls for new drugs against bacterial pathogens. A common approach is to search natural compounds deployed by microbes inhibit competitors. Here, we show that the iron-chelating pyoverdines, siderophores produced environmental Pseudomonas spp., have strong antibacterial properties inducing iron starvation and growth arrest in screen 320 isolates used 12 human pathogens uncovered several pyoverdines with particularly high distinct chemical...
Abstract FUS is a primarily nuclear RNA-binding protein with important roles in RNA processing and transport. mutations disrupting its localization characterize subset of amyotrophic lateral sclerosis (ALS-FUS) patients, through an unidentified pathological mechanism. regulates RNAs, but role at the synapse poorly understood. Here, we used super-resolution imaging to determine physiological extranuclear, neuronal found it predominantly near vesicle reserve pool presynaptic sites. Using...
Abstract The most frequent genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia is a G 4 C 2 repeat expansion in the C9orf72 gene. This gives rise to translation aggregating dipeptide (DPR) proteins, including poly-GA as abundant species. However, gain toxic function effects have been attributed either DPRs or pathological RNA. Here we analyzed cellular model relative toxicity Cytoplasmic aggregates, generated absence RNA, interfered with nucleocytoplasmic protein...
Abstract Hexanucleotide G 4 C 2 repeat expansions in the C9ORF72 gene are most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Dipeptide proteins (DPRs) generated by translation repeat-containing RNAs show toxic effects vivo as well vitro key targets for therapeutic intervention. We human antibodies that bind DPRs with high affinity specificity. Anti-GA engaged extra- intracellular poly-GA reduced aggregate formation a over-expressing cell line....