A5088910380- Protein Structure and Dynamics
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Alzheimer's disease research and treatments
- Computational Drug Discovery Methods
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Cellular transport and secretion
- Protein Interaction Studies and Fluorescence Analysis
- Bioinformatics and Genomic Networks
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Cholinesterase and Neurodegenerative Diseases
- Cardiac electrophysiology and arrhythmias
- HIV/AIDS drug development and treatment
- interferon and immune responses
- Trace Elements in Health
- Microbial Metabolic Engineering and Bioproduction
- Acute Myocardial Infarction Research
- Protein Kinase Regulation and GTPase Signaling
- Biodiesel Production and Applications
- Nanoplatforms for cancer theranostics
- Metabolomics and Mass Spectrometry Studies
- HIV Research and Treatment
- Analytical Chemistry and Chromatography
- Glycosylation and Glycoproteins Research
University of Coimbra
2016-2025
Instituto Pedro Nunes
2017-2025
Hospital de Santo António
2023
Centro Hospitalar do Porto
2023
University of Aveiro
2022
Instituto Português de Oncologia de Coimbra Francisco Gentil
2019
Universidade Federal Rural do Rio de Janeiro
2016
Brandeis University
2015
Division of Chemistry
2015
Biocant
2012
Food processing and storage cause natural food color to diminish, leading the addition of synthetic dyes by industry. Unfortunately, these have potential health risks, such as accumulation in blood plasma interaction with human serum albumin (HSA). To address this issue, present study utilized spectroscopic techniques silico calculations report biophysical profile HSA:Amaranth HSA:New coccine interactions. The showed that HSA:Dye is spontaneous (ΔG° < 0) driven enthalpy (ΔH° ≈ −12 kJ mol–1)...
Amyloid fibril formation and deposition is a common feature of wide range fatal diseases including spongiform encephalopathies, Alzheimer's disease, familial amyloidotic polyneuropathies (FAP), among many others. In certain forms FAP, the amyloid fibrils are mostly constituted by variants transthyretin (TTR), homotetrameric plasma protein. Recently, we showed that in solution may undergo dissociation to non-native monomer, even under close physiological conditions temperature, pH, ionic...
Drug design is an important area of study for pharmaceutical businesses. However, low efficacy, off-target delivery, time consumption, and high cost are challenges can create barriers that impact this process. Deep Learning models emerging as a promising solution to perform de novo drug design, i.e., generate drug-like molecules tailored specific needs. stereochemistry was not explicitly considered in the generated molecules, which inevitable targeted-oriented molecules. This paper proposes...
In amyloidosis, normally innocuous soluble proteins polymerize to form insoluble fibrils. Amyloid fibril formation and deposition have been associated with a wide range of diseases, including spongiform encephalopathies, Alzheimer's disease, familial amyloid polyneuropathies (FAP). certain forms FAP, the fibrils are mostly constituted by variants transthyretin (TTR), homotetrameric plasma protein implicated in transport thyroxine retinol. The most common amyloidogenic TTR variant is...
Crithmum maritimum L. (sea fennel), an edible xerophyte of coastal habitats, is considered emerging cash crop for biosaline agriculture due to its salt-tolerance ability and potential applications in the agri-food sector. Here, nutritional value bioactive properties sea fennel are described. Sea leaves, flowers, schizocarps composed carbohydrates (>65%) followed by ash, proteins, lipids. fennel’s salty, succulent leaves a source omega-6 omega-3 polyunsaturated fatty acids, especially...
Amyloid fibril formation and deposition are the basis for a wide range of diseases, including spongiform encephalopathies, Alzheimer's familial amyloidotic polyneuropathies. However, molecular mechanisms amyloid still poorly characterised. In certain forms polyneuropathy (FAP), fibrils mostly constituted by variants transthyretin (TTR). V30M-TTR is most frequent variant, L55P-TTR variant associated with aggressive form amyloidosis. Here, we report gel filtration chromatography experiments to...
Helichrysum italicum (H. italicum) is a halophyte shrub with bright yellow flowers strong curry-like aroma. The essential oils of H. have been used in the production cosmetics and pharmaceuticals, due to their antiallergic anti-inflammatory properties. In agri-food sector, can be for seasoning flavoring food, as natural food preservatives. Here, we report on composition, bioactive compounds, nutritive value flowers. Flowers were mainly composed carbohydrates (>80 % dry weight), followed by...
Steinernema carpocapsae is an entomopathogenic nematode widely used for the control of insect pests due to its virulence, which mainly attributed ability parasitic stage has overcome defences. To identify mechanisms underlying such a characteristic, we studied novel serpin-like inhibitor (sc-srp-6) that was detected in transcriptome analysis. Recombinant Sc-SRP-6 produced Escherichia coli had native fold serpins belonging α-1-peptidase family and exhibited inhibitory activity against trypsin...
AIP56 (apoptosis-inducing protein of 56 kDa) is a major virulence factor Photobacterium damselae piscicida (Phdp), Gram-negative pathogen that causes septicemic infections, which are among the most threatening diseases in mariculture. The toxin triggers apoptosis host macrophages and neutrophils through process that, vivo, culminates with secondary necrosis apoptotic cells contributing to necrotic lesions observed diseased animals. Here, we show NF-κB p65-cleaving zinc-metalloprotease whose...
A unique feature of plant aspartic proteinase precursors is the presence an internal domain, known as plant-specific insert, whose function not completely understood. The three-dimensional structure insert resembles that saposin-like proteins, a group lipid-binding proteins involved in variety physiological processes. Here we show recombinant able to interact with phospholipid vesicles and induce leakage their contents pH- lipid-dependent manner. activity higher at pH 4.5 requires acidic...
Inherited human long-QT2 syndrome (LQTS) results from mutations in the gene encoding HERG channel. Several LQT2-associated have been mapped to amino terminal cytoplasmic Per-Arnt-Sim (PAS) domain of HERG1a channel subunit. Here we characterized trafficking properties some PAS mutants and analyzed rescue by low temperature (27°C) or pore blocker drug E4031. We show that display molecular are distinct trans-membrane region. Unlike latter, many tested do not result deficiency Moreover, majority...
The kinetics of transthyretin aggregation from acid-unfolded monomers to amyloid fibrils was studied by several biophysical techniques and may be described as a two-step process with transient accumulation oligomeric species.
Supramolecular hydrogels rely on small molecules that self-assemble in water as a result of the cooperative effect several relatively weak intermolecular interactions. Peptide-based low molecular weight hydrogelators have attracted enormous interest owing to simplicity combined with versatility and biocompatibility peptides. In this work, naproxen, well known non-steroidal anti-inflammatory drug, was N-conjugated various dehydrodipeptides give aromatic peptide amphiphiles resist proteolysis....
Success stories of citizen science projects widely demonstrate the value this open paradigm and encourage organizations to shift towards new ways doing research. While benefits for researchers are clear, outcomes individuals participating in these not easy assess. The wide spectrum volunteers collaborating greatly contributes difficulty evaluation projects' outcomes. Given strong links between many education, work we present an experience with hundreds students (aged 15–18) two different...