A5015291771- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Antibiotics Pharmacokinetics and Efficacy
- Pneumonia and Respiratory Infections
- Bacterial biofilms and quorum sensing
- Bacterial Identification and Susceptibility Testing
- Salmonella and Campylobacter epidemiology
- Streptococcal Infections and Treatments
- Antibiotic Use and Resistance
- Bacteriophages and microbial interactions
- Antifungal resistance and susceptibility
- Pharmaceutical and Antibiotic Environmental Impacts
- Urinary Tract Infections Management
- Vibrio bacteria research studies
- Infections and bacterial resistance
- Fungal Infections and Studies
- Mycobacterium research and diagnosis
- Enterobacteriaceae and Cronobacter Research
- Tuberculosis Research and Epidemiology
- Bacterial Genetics and Biotechnology
- Plant Pathogens and Fungal Diseases
- Nosocomial Infections in ICU
- Reproductive tract infections research
- Yersinia bacterium, plague, ectoparasites research
- Bacterial Infections and Vaccines
IASO General Hospital
2016-2021
Iaso Children’s Hospital
2016
National and Kapodistrian University of Athens
2005-2015
Eginition Hospital
2015
General Hospital of Athens G. Genimatas
2009
Essen University Hospital
2009
Oita University
2009
University General Hospital Attikon
2006-2009
Athens Medical Center
1998-2007
Piedmont Athens Regional
1994-2006
Pulsed-fieldgel electrophoresis (PFGE) is the most common genotypic method used in reference and clinical laboratories for typing methicillin-resistant Staphylococcus aureus (MRSA). Many different protocols have been developed that extensive experience with technique established national databases. However, comparabilities of European PFGE MRSA various clones themselves had not addressed until now. This multinational Union (EU) project has first time a database representative epidemic...
To evaluate the prevalence of extended-spectrum beta-lactamase (ESBL)-producing strains among species Enterobacteriaceae, a microdilution susceptibility test was performed with Klebsiella pneumoniae, Escherichia coli, Proteus mirabilis, and Salmonella that were isolated as part SENTRY project. The highest percentage ESBL phenotype (defined minimum inhibitory concentration [MIC] > or =2 microg/mL for ceftazidime, ceftriaxone, aztreonam) detected K. pneumoniae from Latin America (45%),...
Twenty well-characterized isolates of methicillin-resistant Staphylococcus aureus were used to study the optimal resolution and interlaboratory reproducibility pulsed-field gel electrophoresis (PFGE) DNA macrorestriction fragments. Five identical (one PFGE type), 5 that produced related subtypes, 10 with unique patterns analyzed blindly in 12 different laboratories by in-house protocols. In several a standardized protocol commercial kit was applied successfully as well. Eight centers...
ABSTRACT Human brucellosis poses a significant public health problem in many developing countries and requires fast accurate diagnosis. A PCR assay amplifying part of the 31-kDa Brucella abortus antigenic protein gene sequence was developed applied to whole-blood serum samples from 31 patients 45 healthy individuals. All except one had detectable DNA either whole blood or (combined sensitivity, 97%), but sensitivity higher with (94%) than (61%). The specificity excellent (100%). confirmatory...
To investigate the resistance mechanisms and genetic relationship of imipenem-resistant Acinetobacter baumannii isolates recovered in intensive care unit (ICU) a tertiary hospital.Imipenem-resistant A. clinical environmental were collected ICU Red Cross General Hospital, Athens, Greece between March October 2002. The tested by Etest MBL, PCR, RT-PCR sequencing for carbapenemase-encoding genes, PFGE synergy experiments using meropenem efflux pump inhibitor carbonyl cyanide...
We analyzed a representative sample of methicillin-resistant Staphylococcus aureus (MRSA) from 11 European countries (referred to as the HARMONY collection) using three molecular typing methods used within group examine their usefulness for large, multicenter MRSA surveillance networks that use these different laboratory methodologies. isolates were collected based on prevalence in each center and genetic diversity, assessed by pulsed-field gel electrophoresis (PFGE). PFGE groupings (< or =...
Cefiderocol (S-649266), a siderophore cephalosporin, utilizes novel mechanism of entry into the periplasmic space Gram-negative bacteria and is broadly stable to ESBLs carbapenemases.A collection carbapenem-resistant isolated from clinical specimens in 18 Greek hospitals was tested for susceptibility cefiderocol, meropenem, ceftazidime, cefepime, ceftazidime/avibactam, ceftolozane/tazobactam, aztreonam, amikacin, ciprofloxacin, colistin tigecycline. Broth microdilution plates were used...
ABSTRACT Seventeen Klebsiella pneumoniae clinical isolates carrying the bla VIM-1 metallo-β-lactamase gene were collected in intensive care units of three hospitals Athens, Greece, 2002. They exhibited various carbapenem resistance levels (Etest MICs imipenem ranged from 4 to 32 μg/ml). All gave positive results by imipenem-EDTA synergy Etest. The classified into four main types pulsed-field gel electrophoresis; majority (5 and 10 isolates) belonged two types. cassette was part variable...
Although a growing number of reports have described inflammatory bowel disease (IBD) complicated with cytomegalovirus (CMV) infection, there are limited molecular studies that investigate CMV genome in intestinal sections patients IBD.A cross-sectional prospective study was conducted between September 2000 and June 2003 cohort 85 diagnosed IBD (58 ulcerative colitis 27 Crohn's disease) two adult gastrointestinal referral centers Athens, Greece. Prevalence infection estimated by pathologic...
We report the first case of Cryptococcus laurentii meningitis and a rare albidus cryptococcaemia in AIDS patients. Both infections were treated with amphotericin B flucytosine. The C. was controlled after 2 weeks treatment no evidence infection 20 months later. patient cryptococcaemia, despite B/flucytosine combination therapy, died on 14th day treatment. minimum inhibitory concentrations (MICs) for laurentii, as determined by Etest RPMI 1640 agar, 0.25 microg ml(-1) B, 1.25 flucytosine, 4...
Background. BK virus‐associated nephropathy (BKVAN) can be diagnosed only with renal graft biopsy. Definitive diagnosis of BKVAN requires demonstration virus (BKV) replication in allograft tissues. Non‐invasive analysis urine and blood is considered essential screening transplant recipients. Patients methods. This study evaluated prospectively the BKV plasma qualitative quantitative real‐time polymerase chain reaction 32 de novo (group A) 34 chronic B) recipients long‐term impact on...
Abstract In 2004 and 2005, 5 metallo-β-lactamase (MBL)-positive Acinetobacter baumannii isolates were found in 2 Greek hospitals. Isolates unrelated carried blaVIM-1 a class 1 integron; blaOXA-51- blaOXA-58-like carbapenemase genes also detected. VIM-1 MBL spp. causes concern, given the increasing resistance of this species.
Objectives: To investigate the resistance mechanisms of meropenem-resistant, ceftazidime-susceptible Pseudomonas aeruginosa isolates, in a clinical setting where VIM-2 or VIM-4 metallo-β-lactamase (MBL)-producing pseudomonads are common.
Carbapenem resistant Acinetobacter baumannii (CRAB) represents one of the most challenging pathogens in clinical settings. Colistin is routinely used for treatment infections by this pathogen, but increasing colistin resistance has been reported. We obtained 122 CRAB isolates from nine Greek hospitals between 2015 and 2017, those (ColR; N = 40, 32.8%) were whole genome sequenced, also including two susceptible (ColS) comparison. All ColR characterized a previously described mutation,...
ABSTRACT Rapid and efficient epidemiologic typing systems would be useful to monitor transmission of methicillin-resistant Staphylococcus aureus (MRSA) at both local interregional levels. To evaluate the intralaboratory performance interlaboratory reproducibility three recently developed repeat-element PCR (rep-PCR) methods for MRSA, 50 MRSA strains characterized by pulsed-field gel electrophoresis (PFGE) ( Sma I) analysis epidemiological data were blindly typed inter-IS 256 , 16S-23S...
A significant increase in the frequency of isolation Salmonella enteritidis has been observed during recent years Greece, parallelled by an increasing rate resistance this organism to antibiotics. substantial proportion ampicillin- and doxycycline-resistant isolates exhibited cross-resistance drugs other classes, such as sulfonamides streptomycin. Isolates human origin were overall less resistant than those animal or food-feed origin. Indeed, strains associated with infections characterized...
ABSTRACT Twelve Salmonella typhimurium strains resistant to broad-spectrum cephalosporins were isolated from cases of gastroenteritis during 1996 1998 in Russia, Hungary, and Greece. Resistance was due the production CTX-M-type extended-spectrum β-lactamases encoded by similar 12-kb plasmids. By pulsed-field gel electrophoresis, all shared same chromosomal type. These data suggest that an S. clone is present at least three European countries.
The treatment of community-acquired pneumonia is empirical in most cases and must cover a wide range potential pathogens, such as Streptococcus pneumoniae, including penicillin-resistant strains, Haemophilus influenzae intracellular microorganisms. objective this double-blind, randomized, parallel group study was to compare the efficacy safety sparfloxacin (400 mg loading dose, followed by 200 o.d.) with that oral amoxycillin-clavulanic acid (500/125 t.i.d.) or erythromycin (1 g b.i.d.),...
Analysis with a double-disc synergy test (DDST) of clinical isolates Klebsiella pneumoniae and Escherichia coli during the period October 1988-September 1989 revealed that 24% former 4% latter, mainly isolated from urine, possessed an extended-spectrum beta-lactamase. During this no DDST was positive for other enterobacterial species. Transfer ceftazidime resistance demonstrated six K. two E. resistant to third generation cephalosporins aztreonam. Apart one strain pneumoniae, these strains...