A5016409850- Salmonella and Campylobacter epidemiology
- Animal Nutrition and Physiology
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Microbial infections and disease research
- Food Safety and Hygiene
- Meat and Animal Product Quality
- Listeria monocytogenes in Food Safety
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Leptospirosis research and findings
- Livestock and Poultry Management
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Metabolism and Applications
- Molecular Biology Techniques and Applications
- Plant Pathogenic Bacteria Studies
- Herpesvirus Infections and Treatments
- Animal Genetics and Reproduction
- Enterobacteriaceae and Cronobacter Research
- Animal Behavior and Welfare Studies
- Animal Disease Management and Epidemiology
- Biosensors and Analytical Detection
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Mycobacterium research and diagnosis
Brazilian Agricultural Research Corporation
2010-2024
Concordia University Wisconsin
2012-2021
Universidade Federal do Rio Grande do Sul
2004-2018
Pontifícia Universidade Católica do Paraná
2004
The present study analyzes the characteristics of Salmonella spp. from broiler chicken farms in Brazil. In total, 82 strains were characterized by serotyping, determining susceptibility to antimicrobials, and using pulsed-field gel electrophoresis (PFGE). Fifteen serotypes identified, among which Minnesota (40.24%), Infantis (14.63%), Heidelberg (7.31%), Senftenberg (6.09%), Mbandaka (6.09%) most frequent. occurred mostly state Mato Grosso do Sul one companies surveyed. Approximately 60%...
To investigate antimicrobial resistance, 96 Salmonella enterica subspecies serovar Enteritidis strains isolated from salmonellosis outbreaks and poultry-related products obtained in southern Brazil were analyzed. Macrorestriction patterns, by pulsed-field gel electrophoresis phage types, assessed. Although 43.75% of samples sensitive to all drugs tested, resistance sulfonamide (34.37%), trimethoprim-sulfamethoxazole (25.00%), nalidixic acid (14.58%), streptomycin (2.08%), gentamicin,...
Two experiments were undertaken to evaluate the bacterial load in recycled litter between broiler flocks following addition of quicklime (T1), windrowing (T2), shallow fermentation (T3), and control (no intervention, T4). The first experiment was developed field conditions which houses accompanied by 6 consecutive effect treatments assessed on enterobacteria aerobic mesophiles. second conducted an experimental house with for assessment Salmonella Enteritidis phage type 4 (SE PT4). In study,...
Conventional culturing methods enable the detection of Campylobacter in broiler flocks. However, laboratory culture is laborious because its fastidious behavior and presence competing nontarget bacteria. This study evaluated different protocols to isolate from litter, feces, cloacal drag swabs. Samples taken commercial Brazilian flocks were directly streaked onto Preston agar (PA), Campy-Line (CLA), modified charcoal cefoperazone deoxycholate (mCCDA) also enriched blood-free Bolton broth...
A study using sentinel broiler chickens was performed to address Campylobacter persistence in litter that reused for successive flocks. Cloacal swabs, litter, drag darkling beetles, feed, and drinking water were weekly sampled analyzed by standard microbiological procedures. Thermotolerant isolated strains confirmed polymerase chain reaction subtyped pulsed-field gel electrophoresis analysis. not detected samples collected immediately after downtime between However, Campylobacter-positive...
1. This study determined the antimicrobial resistance profile and biofilm-forming ability of Salmonella enterica strains isolated from commercial broiler houses over a three-year period in southern Brazil.2. Of 720 drag swabs analysed, 37 (5%) tested positive for non-typhoidal spp. S. Heidelberg was most frequent serovar.3. Among resistant (83.8%; 31/37), common to tetracycline, ampicillin nalidixic acid. Multidrug found 65% (24/37) isolates, with large proportion multidrug (81%; 13/16).4....
Salmonella enterica subsp. (S.) serovar Enteritidis is one of the main pathogens involved in food-borne diseases worldwide. In epidemiological investigations food-related salmonellosis, subtyping necessary to improve preventive and control measures. Single-enzyme amplified fragment length polymorphism (SE-AFLP) analysis a modified AFLP that uses only restriction enzyme produce DNA fragments are selectively by PCR. order assess applicability SE-AFLP S. typing, hundred eight strains isolated...
Campylobacter is not routinely tested in foodborne disease investigations Brazil. Here, we studied the occurrence of among other food-related bacteria commonly found outbreaks reported Rio Grande do Sul State, Southern Seventy-two food samples were analyzed by using culture-based detection methods during 18-month investigation 36 outbreaks. The sampled foods from all negative for . However, at least one investigated foodborne-related was detected 29.17% (21/72) samples. Taken together, these...
Chicken is a leading source of thermotolerant Campylobacter, which triggers human foodborne enteritis. This study evaluated Campylobacter contamination retail chicken in southern Brazil, using qualitative and quantitative analyses. Selective enrichment Bolton broth for 24 48 h after plating onto modified charcoal–cefoperazone–deoxycholate (mCCD) agar Preston was assessed. The combined results the detection enumeration methods revealed frequency 70% occurrence samples. enumerated 60% samples,...
Salmonella enterica sorovar (S.) Enteritidis é uma das principais bactérias envolvidas em surtos de infecção alimentar humanos decorrentes do consumo produtos origem animal. Nas investigações epidemiológicas, a subtipificação fenotípica e genotípica permitem verificar diferenças ou similaridades entre linhagens, as quais podem indicar sua origem, auxiliando os programas controle prevenção patógeno. No presente trabalho, foram analisados padrões resistência antimicrobianos, fagotipificação,...
A pleuropneumonia suína (PPS) provoca prejuízos significativos na suinocultura no mundo. O agente etiológico é a bactéria Actinobacillus pleuropneumoniae (App), que apresenta 15 sorotipos descritos, os quais variam consideravelmente em relação sua patogenicidade. Nesse sentido, precisa caracterização patotípica desta de grande importância para adoção medidas controle e profilaxia. diagnóstico sorotipificação deste patógeno são realizados pelas técnicas microbiológicas convencionais....
We studied the on-farm variety of thermotolerant Campylobacter strains isolated from field samples received over a 12-mo period four broiler-producing companies in southern Brazil. In total, 515 807 (63.8%) analyzed were found to be Campylobacter-positive. jejuni and C. coli detected; first one being more frequently (98.2%). The highest antimicrobial resistance levels subset 80 against enrofloxacin (89.5%) ciprofloxacin (83.3%). Most 28 DNA macrorestriction profiles identified unique each...