A5038037395- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Cell death mechanisms and regulation
- Phagocytosis and Immune Regulation
- Nerve injury and regeneration
- Immune cells in cancer
- Parkinson's Disease Mechanisms and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Ion Channels and Receptors
- Biomedical Ethics and Regulation
- Thermal Regulation in Medicine
- Telomeres, Telomerase, and Senescence
- Dermatology and Skin Diseases
- Cytokine Signaling Pathways and Interactions
- Glaucoma and retinal disorders
- Mast cells and histamine
- Biochemical Analysis and Sensing Techniques
- Retinal Diseases and Treatments
- RNA regulation and disease
- Respiratory and Cough-Related Research
- Virus-based gene therapy research
Boehringer Ingelheim (India)
2025
Boehringer Ingelheim (Germany)
2017-2024
Novartis Institutes for BioMedical Research
2011
Novartis (Switzerland)
2011
Heidelberg University
2000-2005
German Cancer Research Center
2000-2005
Interactions between the immune system and malignant cells play an important role in tumorigenesis. Failure of to detect reject transformed may lead cancer development. Tumors use multiple mechanisms escape from immune-mediated rejection. Many these are now known on a cellular molecular level. Despite this knowledge, immunotherapy is still not established treatment clinic. This review discusses used by tumors with emphasis related apoptosis.
Progressive accrual of senescent cells in aging and chronic diseases is associated with detrimental effects tissue homeostasis. We found that fibroblasts epithelia were not only refractory to macrophage-mediated engulfment removal, but they also paralyzed the ability macrophages remove bystander apoptotic corpses. Senescent cell-mediated efferocytosis suppression (SCES) was independent senescence-associated secretory phenotype (SASP) instead required direct contact between cells. SCES...
Hepatocellular carcinoma (HCC) and solid cancers with liver metastases are indications high unmet medical need. Interleukin-12 (IL-12) is a proinflammatory cytokine substantial anti-tumor properties, but its therapeutic potential has not been realized due to severe toxicity. Here, we show that orthotopic tumors in mice can be treated by targeting hepatocytes via systemic delivery of adeno-associated virus (AAV) vectors carrying the murine IL-12 gene. Controlled production was achieved vivo...
<title>Abstract</title> Macrophages are the major immune cell population in most human solid cancers. Therefore, we hypothesized that appropriate activation of macrophages tumors could convert an immunosuppressive into a proinflammatory tumor microenvironment and, thus, enable T cell-mediated killing cells. To identify activating receptors, selected putative agonists from literature and used them for stimulation primary monocyte-derived as single agents or combination. Stimulated were...
The ocular half-life of intravitreally (IVT) injected drugs is major relevance for the suitability a drug intended chronic intraocular treatment, as determines dosing frequency. Thus, extension principles are very attractive they can reduce IVT In this study, we investigated pharmacokinetics (PK) Nanobody® BI-X and whether noncovalent binding to vitreous albumin could increase its half-life.Wistar rats were dosed with 3 μg alone or coadministered human serum (HSA), exposure was measured in...
CD95 (APO-/Fas) ligand (CD95L) is a member of the TNF family predominantly expressed by activated T and NK cells but also tumors diverse cellular origin. CD95L trimerizes surface target that subsequently undergo apoptosis. The role CD95/CD95L system in down-regulation an immune response (activation-induced cell death) established. However, it so far unclear why express CD95L. To investigate whether use to down-regulate anti-tumor response, we established transgenic (tg) mouse model...
Abstract Many tumors express CD95L (CD178, FasL, APO‐1L) and may thus kill tumor‐infiltrating lymphocytes, a phenomenon called tumor counterattack. However, presently it is not clear whether counterattack relevant immune escape mechanism. To characterize the effect of expression cells on tumor‐specific T cells, we established an in vitro system with TCR tg model antigen. Preactivated antitumor were able to − + cells. killed activated inhibited expansion cytotoxic mixed lymphocyte reactions....
Regulatory T cells (Treg) play a critical role in controlling immune responses diseases such as cancer or autoimmunity. Activated Treg express the membrane protein GARP (LRRC32) complex with latent form of immunosuppressive cytokine TGF-β (L-TGF-β). In this study, we confirmed that active was generated from its an integrin-dependent manner and induced receptor signaling activated human Treg. We studied series Abs targeting L-TGF-β/GARP distinct binding modes. found could be inhibited by...
Many tumors express the death ligand CD95L (CD178, APO-1L, FasL) and can kill activated T cells in vitro. This may enable tumor to suppress anti-tumor immune responses, a phenomenon called "tumor counterattack". Preliminary evidence of counterattack human exists. However, CD95L-expressing are rapidly rejected mice. In order clarify this controversial situation we investigated whether level or time point expression might be critical factors determining versus rejection. We generated...
Prion-like transmission of pathology in α-synucleinopathies like Parkinson's disease or multiple system atrophy is increasingly recognized as one potential mechanism to address progression. Active and passive immunotherapies targeting insoluble, aggregated α-synuclein are already being actively explored the clinic with mixed outcomes so far. Here, we report identification 306C7B3, a highly selective, aggregate-specific antibody picomolar affinity devoid binding monomeric, physiologic...
There is a rising need for biomaterial in dermatological research with regard to both quality and quantity. Research biobanks as organized collections of biological material associated personal clinical data are increasing importance. Besides technological/methodological legal aspects, the willingness donate samples by patients healthy volunteers key success factor. To analyze theoretical blood skin samples, we developed distributed questionnaire. Six hundred nineteen questionnaires were...
Inflammatory skin diseases have a high prevalence in Western countries and pharmaceutical companies spend increasing amounts of money to develop drugs for these disorders. However, their complex pathophysiology is only partially reflected classical rodent models, limiting predictivity, new compounds frequently fail clinical trials. Therefore, there an urgent need more predictive reliable animal models. Humanised mouse models seem combine the convenience small with good correlation situation...
One important prerequisite for developing a therapeutic monoclonal antibody is to evaluate its in vivo efficacy. We tested the potential of an anti-CD96 alone or combination with anti-PD-1 mouse colon cancer model. Early treatment significantly decreased tumor growth and further increased benefit, while had no effect. In late settings, resulted enhanced CD8+ T cell infiltration tumors CD8/Treg ratio. Measured concentrations were as expected animals treated alone, but lower at later time...
Abstract Prion-like transmission of pathology in α-synucleinopathies like Parkinson’s disease or multiple system atrophy is increasingly recognized as one potential mechanism to address progression. Active and passive immunotherapies targeting insoluble, aggregated α-synuclein are already being actively explored the clinic with mixed outcomes so far. Here, we report identification 306C7B3, a highly selective, aggregate-specific antibody picomolar affinity devoid binding monomeric,...