A5000712798- Vector-borne infectious diseases
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Vector-Borne Animal Diseases
- Genomics and Phylogenetic Studies
- Bartonella species infections research
- Plant and Fungal Interactions Research
Suffolk University
2017-2019
Ticks carry a wide range of known human and animal pathogens are postulated to others with the potential cause disease. Here we report discovery effort wherein unbiased high-throughput sequencing was used characterize virome 2,021 ticks, including Ixodes scapularis (n = 1,138), Amblyomma americanum 720), Dermacentor variabilis 163), collected in New York, Connecticut, Virginia 2015 2016. We identified 33 viruses, 24 putative novel viral species. The most frequently detected viruses were...
We employed high throughput sequencing to survey the microbiomes of Ixodes scapularis collected in New York and Connecticut. examined 197 individual I. adults pools from 132 nymphs. detected Borrelia burgdorferi sensu stricto 56.3% ticks, Anaplasma phagocytophilum 10.6%, miyamotoi 5%, Babesia microti 7.6%, Powassan virus 3.6%. did not detect mayonii, Ehrlichia muris eauclairensis, Bartonella spp. or pathogenic species other than B. microti. The most abundant bacterium (65%), only rickettsial...
The understanding of pathogen prevalence is an important factor in the determination human risks for tick-borne diseases and can help guide diagnosis treatment. implementation our assay addresses a critical need surveillance diseases, through generation comprehensive assessment I. scapularis . Our finding high frequency ticks infected with Babesia microti Suffolk County, NY, implicates this agent as probable frequent cause non-Lyme disease area.
A mixed infection of a single tick or host by Lyme disease spirochetes is common and unique challenge for the diagnosis, treatment, surveillance disease. Here, we describe novel protocol differentiating strains on basis deep sequencing hypervariable outer surface protein C locus (ospC). Improving upon traditional DNA-DNA hybridization method, next-generation sequencing-based high throughput, quantitative, able to detect new pathogen strains. We applied method more than one hundred infected...
Abstract Mixed infection of a single tick or host by Lyme disease spirochetes is common and unique challenge for diagnosis, treatment, surveillance disease. Here we describe novel protocol differentiating strains based on deep sequencing the hypervariable outer-surface protein C locus ( ospC ). Improving upon traditional DNA-DNA hybridization method, next-generation sequencing-based high-throughput, quantitative, able to detect new pathogen strains. We applied method over one hundred...