A5051917256- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Health and Technologies
- Ovarian function and disorders
- Assisted Reproductive Technology and Twin Pregnancy
- Hormonal and reproductive studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sexual Differentiation and Disorders
- Reproductive biology and impacts on aquatic species
- Genital Health and Disease
- Effects and risks of endocrine disrupting chemicals
- Reproductive Physiology in Livestock
- Hepatitis B Virus Studies
- Healthcare Systems and Technology
- BRCA gene mutations in cancer
- Geochemistry and Elemental Analysis
- Mercury impact and mitigation studies
- Reproductive System and Pregnancy
- Pharmacological Effects and Assays
- Organic Chemistry Cycloaddition Reactions
- Marine Biology and Environmental Chemistry
- Electronic Health Records Systems
- Environmental DNA in Biodiversity Studies
- Heavy metals in environment
- Bioenergy crop production and management
Antwerp University Hospital
2012-2023
University of Antwerp
2019-2023
Ghent University Hospital
1982-1984
Summary Semen analysis is difficult to standardize, quality control and assurance are necessary ensure that results accurate precise. This Belgian EQA survey over a 15‐year period, involving 121 laboratories, attempted reduce interlaboratory variability at the same time, encouraged participating laboratories implement correct techniques as advised by WHO. Over total median coefficient of variation (CV) for sperm count, irrespective method used was 19.2%, while using improved Neubauer chamber...
PROBLEM: The potential value of assessment cytokine concentrations for the diagnosis certain pathological conditions male reproduction has not been fully evaluated. METHOD: interleukin 6 (IL‐6, pg/mL), its soluble receptor (IL‐6 sR, ng/mL), and 1β (IL‐1β, pg/mL) have measured in semen samples 114 men 12 corresponding blood sera. RESULTS: concentration IL‐6 was unrelated to that receptor. Both sR were higher first (mean: 69 31 resp.) than second fraction (39 13) split ejaculates, within...
Both plasma 5α-androstane-3α,17β-diol (3αdiol) concentrations and urinary 5α-androstane-3α,17β-diol-glucuronide (3αdiol G) as well 5β-androstane-3α,17β-diol-glucuronide (5β-3αdiol excretion levels were measured in 27 males 22 females. To study the influence of age on these values, either 3αdiol or 3α- 5β-3αdiol G determined an additional number subjects. significantly higher young (<30 yr old; 25.1 ± 1.5 ng/dl) than elderly (>60 12.6 1.2 (3.3 0.4 postmenopausal women (2.4 ng/dl). Similarly,...
Sperm morphology has always been considered an important tool in evaluating a man's fertilizing potential. The objective of this multicentric study was to evaluate intra- and interindividual variability between-laboratory variation using the same or different criteria sperm assessment. Semen samples were obtained from 20 males 32 smears made all samples. Eighty coded (4 per patient) sent 8 laboratories for centers applied classification systems (strict criteria, WHO 1987, Dusseldorf...
Journal Article Comparison between different pre-treatment techniques for sperm recovery prior to intrauterine insemination, GIFT or IVF Get access U. Punjabi, Punjabi 1 Fertility Clinic, University Hospital of Antwerp2520 Edegem, Belgium 1To whom correspondence should be addressed Search other works by this author on: Oxford Academic PubMed Google Scholar J. Gerris, Gerris J.van Bijlen, Bijlen L. Delbeke, Delbeke M. Gielis, Gielis Ph. Buytaert Human Reproduction, Volume 5, Issue 1, January...
Endogenous and exogenous factors can severely affect the integrity of genetic information by inducing DNA damage impairing genome stability. The extent to which men with without subfertility are exposed several adverse lifestyle impact on sperm fragmentation (SDF), chromatin maturity (condensation decondensation), stability (hypo- hypercondensation) aneuploidy assessed in this study. Standardized assays employing flow cytometry were used detect instability 556 samples. Semen parameters...
The present study evaluated the effects of field metal contamination on sperm motility and RNA/DNA ratio in echinoderms. Populations Asterias rubens Echinus acutus that occur naturally along a gradient sediments by cadmium, copper, lead, zinc Norwegian fjord (the Sørfjord) were studied. Sperm motility, measure quality, was quantified using computer-assisted analysis system. ratio, protein synthesis, assessed one-dye (ethidium bromide)/one-enzyme (RNase), 96-well microplate fluorometric...
A recent meta-regression analysis reported a temporal trend in sperm count showing significant decline between 1973 and 2011. This is thought to affect fecundity. Moreover, semen quality considered of key interest public health given its association with all-cause male morbidity/mortality. The issue requires ongoing investigation due geographical variation methodological errors analysis.To study whether there Belgian candidate donors donors' fertility potential.Retrospective samples provided...
Semen parameters are unable to inform on the function or fertilizing capacity of male gamete. Standardized methods provided by WHO but, lower reference limits have reduced sensitivity predict chances conception. Subfertile men may be falsely classified as “normal” and a factor contributing genome instability overlooked. parameters, sperm DNA fragmentation (SDF), chromatin maturity stability, aneuploidy were assessed in fertile (F), subfertile normozoospermic (SN) non-normozoospermic males...
Antioxidant therapy should be reserved for infertile patients who actually exhibit signs of oxidative stress (OS). Nevertheless, there is no consensus regarding the measure primary endpoint and assay that used. The formation 8-hydroxy-2′-deoxyguanosine (8-OHdG), an early marker sperm DNA oxidation (SDO), was analyzed using flow cytometry, in men at a University hospital setup infertility treatment. Similar to conventional semen parameters, 8-OHdG validated on fresh samples reduce variability...
To validate semen preparation via density gradient centrifugation, we took into account the input sample, action generated by technical and equipment characteristics output measured level of performance. Longer periods abstinence reduced % yield, but increased viscosity incomplete samples collected had no effect. Under controlled characteristics, precision reproducibility were validated for gradient. Additionally, as a good laboratory practice, internal external quality control measures...
Journal Article The value of intrauterine insemination with washed husband's sperm in the treatment infertility Get access Jan M. Gerris, Gerris 1 Department Gynaecology and Obstetrics, University Hospital AntwerpWilrijkstraat, 10, Edegem, Belgium 1To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Luc O. Delbeke, Delbeke Usha Punjabi, Punjabi Philippe Buytaert Human Reproduction, Volume 2, Issue 4, May 1987, Pages...
The biological variability of semen and sperm DNA fragmentation (SDF) parameters in a longitudinal intrauterine insemination (IUI) trial over multiple IUI cycles was investigated. A TUNEL assay used for SDF testing, both before after density gradient centrifugation. significant age effect observed: while deteriorated with advancing age, on average, higher values were observed older males. There quite some variables. Using fertile threshold values, three patient categories distinguished:...