A5087880318- Ichthyology and Marine Biology
- Fish Ecology and Management Studies
- Fish biology, ecology, and behavior
- Fish Biology and Ecology Studies
- Genomics and Phylogenetic Studies
- Paleontology and Evolutionary Biology
- Advanced biosensing and bioanalysis techniques
- Virtual Reality Applications and Impacts
- Marine Ecology and Invasive Species
- Action Observation and Synchronization
- Parasitic infections in humans and animals
- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Insect and Arachnid Ecology and Behavior
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Osaka Science Museum
2017-2025
Enoshima Aquarium
2023
Modern cartilaginous fishes are divided into elasmobranchs (sharks, rays and skates) chimaeras, the lack of established whole-genome sequences for former has prevented our understanding early vertebrate evolution unique phenotypes elasmobranchs. Here we present de novo assemblies brownbanded bamboo shark cloudy catshark an improved assembly whale genome. These relatively large genomes (3.8-6.7 Gbp) contain sparse distributions coding genes regulatory elements exhibit reduced molecular...
Background: Studying cartilaginous fishes (chondrichthyans) has helped us understand vertebrate evolution and diversity. However, resources such as genome sequences, embryos, detailed staging tables are limited for species within this clade. To overcome these limitations, we have focused on a species, the brownbanded bamboo shark ( Chiloscyllium punctatum ), which is relatively common aquarium that lays eggs continuously throughout year. In addition, because of its small size, promising...
Karyotyping, traditionally performed using cytogenetic techniques, is indispensable for validating genome assemblies whose sequence lengths can be scaled up to chromosome sizes modern methods. Karyotype reports of chondrichthyans are scarce because the difficulty in cell culture. Here, we focused on carpet shark species and culture conditions fibroblasts lymphocytes. The utility cultured cells enabled high-fidelity characterization their karyotypes, namely 2n = 102 whale (Rhincodon typus)...
<ns4:p>The taxon Elasmobranchii (sharks and rays) contains one of the long-established evolutionary lineages vertebrates with a tantalizing collection species occupying critical aquatic habitats. To overcome current limitation in molecular resources, we launched Squalomix Consortium 2020 to promote genome-wide array approaches, specifically targeting shark ray species. Among various bottlenecks working elasmobranchs are their elusiveness low fecundity as well large highly repetitive genomes....
ABSTRACT Little is known about the reproductive biology of endangered spinetail devil ray ( Mobula mobular ). Here, we describe behavior, estimates age, size at maturity, embryonic development in uterine cavity, and morphology a neonate produced by male female kept Osaka Aquarium KAIYUKAN for 7.5 years. The behaviors observed included initiation, endurance, evasion, pre‐copulation positioning, copulation, separation. disc widths (DW) rays displaying were 203 204 cm, respectively. was...
Sex determination has been investigated across vertebrate lineages to reveal stepwise evolution of sex chromosomes and diversity responsible molecular mechanisms. However, these studies hardly encompass cartilaginous fishes deeply isolated from the rest vertebrates, which hinders comprehensive view determination. Here, we produced chromosome-scale genome assemblies egg-laying shark species comparatively sequences transcriptome profiles diverse fishes. chromosome identification, supported by...
Computed tomography (CT) is used in veterinary medicine for the diagnosis of bones and soft tissue diseases various species. In addition, CT has recently been to diagnose aquatic animals, including Selachimorpha, which are difficult out water. However, because Selachimorpha do not have adipose coelomic cavity, organs cannot be fully identified using non-contrast (NCCT). The aim this study present anatomical features cadaver, NCCT, contrast-enhanced (CECT) as well change values musculature...
Abstract The pyjama shark ( Poroderma africanum ) is a Scyliorhinid species endemic to South Africa. Even though it kept in captivity many aquariums, there lack of research on its growth and development. In this study, we investigated the fertilization rate eggs, growth, age at female sexual maturation captive sharks, compared their that wild individuals. 319 eggs was 18.8%, total length L t body weight W hatching were 14.65 ± 0.26 cm 16.6 0.8 g, respectively. Both wild, males showed...
Abstract The pyjama shark ( Poroderma africanum ) is a Scyliorhinid species endemic to South Africa. Even though it kept in captivity many aquaria, there lack of research on its growth and development. In this study, we investigated the fertilization rate eggs age at female sexual maturation captive sharks compared their that wild individuals. This first study compare catsharks as well male benefits from much larger sample size than has previously been collected. mean incubation (±standard...
a porcupinefish, Diodon nichthemerus Cuvier (Tetraodontiformes: Diodontidae), that had been imported from South Australia and raised at Osaka Aquarium Kaiyukan in Osaka, Japan, was found to harbour parasitic lesion the upper part of subcutaneous tissue surrounding left eye D. (Fig. 1A).After anesthetising fish by 2-phenoxyethanol, three nematodes were surgically extracted lesion; they milky colour, flexible, slender string-shaped 1B).These then fixed 70% ethanol stained with lactophenol...
Abstract Karyotyping is indispensable for validating genome assemblies whose sequence lengths can be scaled up to chromosome sizes using modern methods and traditionally performed cytogenetic techniques. Karyotype reports of chondrichthyans are scarce, mainly because their unique osmoregulatory mechanism, which hinders cell culture. Here, we focused on carpet shark species the culture conditions fibroblasts lymphocytes. Using this method, high-fidelity characterization karyotypes, namely 2n...