A5040030161- Evolution and Genetic Dynamics
- DNA Repair Mechanisms
- Aquatic Ecosystems and Phytoplankton Dynamics
- Algal biology and biofuel production
- Environmental Toxicology and Ecotoxicology
- Plant Virus Research Studies
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Biocrusts and Microbial Ecology
- Bacteriophages and microbial interactions
- Heavy metals in environment
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Pesticide and Herbicide Environmental Studies
- Parasitic Infections and Diagnostics
- Protein Structure and Dynamics
- Bacillus and Francisella bacterial research
- Bacterial biofilms and quorum sensing
- Diatoms and Algae Research
- Misinformation and Its Impacts
- Cancer Genomics and Diagnostics
- Toxoplasma gondii Research Studies
- Animal Ecology and Behavior Studies
- Water Quality Monitoring and Analysis
- Pharmaceutical and Antibiotic Environmental Impacts
National Institute of Environmental Health Sciences
2010-2024
Baylor College of Medicine
2019-2023
Dan L Duncan Comprehensive Cancer Center
2018
National Institutes of Health
2013
Universidad Complutense de Madrid
2001-2006
Evolution of antibiotic resistance is a world health crisis, fueled by new mutations. Drugs to slow mutagenesis could, as cotherapies, prolong the shelf-life antibiotics, yet evolution-slowing drugs and drug targets have been underexplored ineffective. Here, we used network-based strategy identify that block hubs fluoroquinolone antibiotic-induced mutagenesis. We U.S. Food Drug Administration– European Medicines Agency–approved drug, dequalinium chloride (DEQ), inhibits activation...
Neospora caninum, a recently recognized protozoan parasite of animals, is considered to be major cause bovine abortion worldwide. Although its life cycle not completely known, recent studies suggest that the sexual stage occurs in dogs. The prevalence reproduction N. however, unknown. We investigated ability 3 caninum isolates (NC-1, NC-SweB1, and NC-Liverpool) propagate asexually for approximately 250 generations cell line which they had been cultured previously. malthusian parameter...
Abstract Pseudomonas aeruginosa is a major cause of nosocomial infections and the leading chronic lung in cystic fibrosis obstructive pulmonary disease patients. Antibiotic treatment remains challenging because P. resistant to high concentrations antibiotics has remarkable ability acquire mutations conferring resistance multiple groups antimicrobial agents. Here we report that when plated on ciprofloxacin (cipro) plates, majority cipro-resistant (ciproR) colonies observed at after 48 h...
Residues contribute to water pollution and pose a challenge microalgal populations because numerous contaminants are toxic microalgae, even in the micromolar range. Adaptation of microalgae from herbicide sensitivity resistance was analysed by an ecological genetic approach, using unicellular alga Dictyosphaerium chlorelloides (Chlorophyceae) as experimental model. A dose–effect study showed that Malthusian parameter under conditions r selection uncrowded environment carrying capacity K...
The increasing rates of global extinction due to human activities necessitate studies the ability organisms adapt new environmental conditions resulting from disturbances. We investigated evolutionary adaptation a microalga sudden change exposure novel toxic chemical residues. A laboratory strain Dictyosphaerium chlorelloides (Naum.) Kom. and Perm. (Chlorophyceae) was exposed concentrations modern contaminant 2,4,6‐trinitrotoluene (TNT). When algal cultures were 30 mg·L − 1 TNT, massive...
Riboviruses (RNA viruses without DNA replication intermediates) are the most abundant pathogens infecting animals and plants. Only a few riboviral infections can be controlled with antiviral drugs, mainly because of rapid appearance resistance mutations. Little reliable information is available concerning i) kinds relative frequencies mutations (the mutational spectrum), ii) mode genome mutation accumulation, iii) rates spontaneous mutation. To illuminate these issues, we developed model in...
The toxic spill of acid wastes rich in heavy metals/metalloids (AWHM) from the Aznalcóllar mine April 1998, threatening Doñana National Park, is considered to be worst environmental disaster related acute pollution Spanish history. aim this work was study adaptation microalgae (which play an important role as primary producers) AWHM sensitivity resistance by using alga Scenedesmus intermedius experimental model. Malthusian parameter (m) and carrying capacity (K) were reduced mud soil samples...
Adaptation of Spirogyra insignis (Chlorophyceae) to growth and survival in an extreme natural environment (sulphureous waters from La Hedionda Spa, S. Spain) was analysed by using experimental model. Photosynthesis the alga were inhibited when it cultured Spa (LHW), but after further incubation for several weeks, culture survived due a variant that resistant LHW. A Luria-Delbruck fluctuation analysis carried out distinguish between filaments arising rare spontaneous mutations other...
Understanding virus evolution is key for improving ways to counteract virus-borne diseases. Results from comparative analyses have previously suggested a trade-off between fecundity and lifespan viruses that infect the bacterium Escherichia coli (i.e. coliphages), which, if confirmed, would define particular constraint on of fecundity. Here, occurrence such investigated through selection experiment using coliphage Qß. Selection was applied increased in three independent wild-type Qß...
V. López-Rodas, E. Costas, Bañares, L. García-Villada, M. Altamirano, Rico, C. Salgado and A. Flores-Moya. 2006. Analysis of polygenic traits Microcystis aeruginosa (Cyanobacteria) strains by restricted maximum likelihood (REML) procedures: 2. Microcystin net production, photosynthesis respiration. Phycologia 45: 243–248. DOI: 10.2216/04-31.1Phenotypic expression is the result complex interactions between genotype environment. A combination experiments statistics based on procedures was...
RESUMEN. Evolucion fenotípica en microalgas: un cambio morfológico espectacular Dictyosphaerium chlorelloides (Chlorophyta) tras la exposición a TNT. Un muy rápido tiene lugar de cepas salvajes al potente alguicida 2,4,6 trinitrotolueno (TNT). Las salvajes, sensibles TNT, tienen una morfología típica esférico-elipsoidal. Tras exponer dichas dosis letales solamente sobreviven células resistentes tóxico cuya silueta es fusiforme. Estos fusiformes aparecen como consecuencia mutaciones...
SUMMARY Antibiotics can induce mutations that cause antibiotic resistance. Yet, despite their importance, mechanisms of antibiotic-promoted mutagenesis remain elusive. We report the fluoroquinolone ciprofloxacin (cipro) induces drug resistance by triggering differentiation a mutant-generating cell subpopulation, using reactive oxygen species (ROS) to signal sigma-S (σ S ) general-stress response. Cipro-generated DNA breaks activate SOS DNA-damage response and error-prone polymerases in all...
SUMMARY Antibiotics can induce mutations that cause antibiotic resistance, triggered by stress responses. The identities of the stress-response activators reveal environmental cues elicit mutagenesis, and are weak links in mutagenesis networks, inhibition which could slow evolution resistance during therapies. Despite pivotal importance, few fewer functions responses clear. Here, we identify stringent starvation response fluoroquinolone-antibiotic ciprofloxacin (stress)-induced mutagenesis....