Elisa Zago
A5038226483- Acute Myeloid Leukemia Research
- Horticultural and Viticultural Research
- Plant responses to water stress
- Cancer Genomics and Diagnostics
- Plant Reproductive Biology
- Fermentation and Sensory Analysis
- Mast cells and histamine
- Mitochondrial Function and Pathology
- Hemoglobin structure and function
- Legume Nitrogen Fixing Symbiosis
- Multiple Myeloma Research and Treatments
- Parkinson's Disease Mechanisms and Treatments
- Plant Diversity and Evolution
- Plant Physiology and Cultivation Studies
- Plant biochemistry and biosynthesis
- Plant-Microbe Interactions and Immunity
- Banana Cultivation and Research
- Nuts composition and effects
- Photosynthetic Processes and Mechanisms
- Chronic Myeloid Leukemia Treatments
- Plant Pathogenic Bacteria Studies
- Click Chemistry and Applications
- Plant Pathogens and Fungal Diseases
- Nuclear Receptors and Signaling
- RNA Interference and Gene Delivery
University of Verona
2004-2020
Ghent University
2006
Universidade Estadual de Campinas (UNICAMP)
2000
Abstract With approximately 450 species, spiny Solanum species constitute the largest monophyletic group in Solanaceae family, but a high-quality genome assembly from this is presently missing. We obtained chromosome-anchored of eggplant ( melongena ), containing 34,916 genes, confirming that diploid gene number around 35,000. Comparative genomic studies with tomato S . lycopersicum potato tuberosum ) and pepper Capsicum annuum highlighted rapid evolution miRNA:mRNA regulatory pairs R-type...
Nitric oxide (NO) is a widespread signaling molecule, and numerous targets of its action exist in plants. Whereas the activity NO erythrocytes, microorganisms, invertebrates has been shown to be regulated by several hemoglobins, function plant hemoglobins detoxification not yet elucidated. Here, we show that Arabidopsis thaliana nonsymbiotic hemoglobin AHb1 scavenges through production S-nitrosohemoglobin reduces emission under hypoxic stress, indicating role detoxification. However, does...
Nitric oxide (NO) and hydrogen peroxide (H(2)O(2)) are regulatory molecules in various developmental processes stress responses. Tobacco (Nicotiana tabacum) leaves exposed to moderate high light dramatically potentiated NO-mediated cell death catalase-deficient (CAT1AS) but not wild-type plants, providing genetic evidence for a partnership between NO H(2)O(2) during the induction of programmed death. With this experimental model system, specific impact on gene expression was characterized by...
The grapevine (Vitis vinifera) cultivar Tannat is cultivated mainly in Uruguay for the production of high-quality red wines. berries have unusually high levels polyphenolic compounds, producing wines with an intense purple color and remarkable antioxidant properties. We investigated genetic basis these important characteristics by sequencing genome Uruguayan clone UY11 using Illumina technology, followed a mixture de novo assembly iterative mapping onto PN40024 reference genome. RNA data...
Plants such as grapevine (Vitis spp.) display significant inter-cultivar genetic and phenotypic variation. The components underlying diversity in must be understood order to disentangle environmental factors.We have shown that cDNA sequencing by RNA-seq is a robust approach for the characterization of varietal between local cultivar (Corvina) PN40024 reference genome. We detected 15,161 known genes including 9463 with novel splice isoforms, identified 2321 potentially protein-coding...
Fruits stored at low temperature can exhibit different types of chilling injury. In apple, one the most serious physiological disorders is superficial scald, which characterized by discoloration and brown necrotic patches on fruit exocarp. Although this phenomenon widely ascribed to oxidation α-farnesene, its physiology not yet fully understood. To elucidate mechanism scald development possible means prevention, we performed an integrated metabolite screen, including analysis volatiles,...
Post-harvest withering of grape berries is used in the production dessert and fortified wines to alter must quality characteristics increase concentration simple sugars. The molecular processes that occur during are poorly understood, so a detailed transcriptomic analysis post-harvest was carried out by AFLP-transcriptional profiling analysis. This will help elucidate mechanisms berry provide an opportunity select markers can be follow drying process evaluate different techniques. AFLP-TP...
Abstract Advanced age represents one of the major risk factors for Parkinson’s Disease. Recent biomedical studies posit a role microRNAs, also known to be remodelled during ageing. However, relationship between microRNA remodelling and ageing in Disease, has not been fully elucidated. Therefore, aim present study is unravel relevance microRNAs as biomarkers Disease within framework. We employed Next Generation Sequencing profile serum from samples informative (recently diagnosed, drug-naïve)...
Abstract Parkinson’s disease (PD) is the neurological disorder showing greatest rise in prevalence from 1990 to 2016. Despite clinical definition criteria and a tremendous effort develop objective biomarkers, precise diagnosis of PD still unavailable at early stage. In recent years, an increasing number studies have used omic methods unveil molecular basis PD, providing detailed characterization potentially pathological alterations various biological specimens. Metabolomics could provide...
Non-small cell lung cancer (NSCLC) accounts for 81% of all cases and they are often fatal because 60% the patients diagnosed at an advanced stage. Besides need earlier diagnosis, there is a high additional effective therapies. In this work, we investigated feasibility progression mouse model, mimicking features human aggressive NSCLC, as biological reservoir potential therapeutic targets biomarkers. We performed RNA-seq profiling on total RNA extracted from lungs 30 week-old...
Acetoacetate, an NADH oxidant, stimulated the ruthenium red‐insensitive rat liver mitochondrial Ca 2+ efflux without significant release of state‐4 respiration, disruption membrane potential (Δ ψ ) or swelling. This process is compatible with opening currently designated low conductance state permeability transition pore (PTP) and, under our experimental conditions, was associated a partial oxidation pyridine nucleotides. In contrast, diamide, thiol induced fast Δ and large amplitude high...
Investigation of tomato genetic resources is a crucial issue for better straight evolution and studies as well breeding strategies. Traditional Vesuviano San Marzano varieties grown in Campania region (Southern Italy) are famous their remarkable fruit quality. Owing to economic social importance understand the basis unique traits. Here, we present draft genome sequences genome. A 40x coverage was obtained from hybrid Illumina paired-end reads assembling that combines de novo assembly with...
The molecular basis of advanced systemic mastocytosis (SM) is not fully understood and despite novel therapies the prognosis remains dismal. Exome sequencing an index-patient with mast cell leukemia (MCL) uncovered biallelic loss-of-function mutations in SETD2 histone methyltransferase gene. Copy-neutral loss-of-heterozygosity at 3p21.3 (where maps) was subsequently found SM patients prompted us to undertake in-depth analysis copy number, mutation status, transcript expression methylation...
Abstract Recent literature shows that loss of replicative ability and acquisition a proinflammatory secretory phenotype in senescent cells is coupled with the build-in nucleic acids cytoplasm. Its implication human age-related diseases under scrutiny. In endothelial (ECs), we assessed accumulation intracellular during vitro senescence after exposure to high glucose concentrations, which mimic an vivo condition hyperglycemia. We showed induces senescent-like features ECs, including telomere...
Olaparib is effective in metastatic triple negative breast cancer (TNBC) carrying germline mutations DNA damage repair (DDR) genes BRCA1/2 (gBRCA-mut). The OLTRE window-of-opportunity trial preliminarily investigated potential pathologic, radiometabolic and immune biomarkers of early-response to olaparib gBRCA-wild-type (wt) TNBC and, as proof-of-concept gBRCA-mut HER2-negative BC.Patients received for 3 weeks (3w) before standard neoadjuvant chemotherapy underwent multiple FDG18-PET/CT scan...
Advanced age is the major risk factor for idiopathic Parkinson's disease (PD), but to date biological relationship between PD and ageing remains elusive. Here we describe rationale design of H2020 funded project "PROPAG-AGEING", whose aim characterize contribution process development. We summarize current evidences that support existence a continuum justify use Geroscience approach study PD. focus in particular on role inflammaging, chronic, low-grade inflammation characteristic elderly...
L., known as the Jerusalem artichoke, is a hexaploid plant species, adapted to low-nutrient soils, that accumulates high levels of inulin in its tubers. Inulin fructose-based polysaccharide used either dietary fiber or for production bioethanol. Key enzymes involved biosynthesis are well known. However, gene networks underpinning tuber development and accumulation
Polyploidization as the consequence of 2n gamete formation is a prominent mechanism in plant evolution. Studying its effects on genome, and genome expression, has both basic applied interest. We crossed two diploid (2n = 2x 16) Medicago sativa plants, subsp. falcata seed parent, coerulea × pollen parent that form mixture n eggs pollen, respectively. Such cross produced full-sib tetraploid 4x 32) hybrids, latter being result bilateral sexual polyploidization (BSP). These unique materials...
Abstract A prodromal phase of Parkinson’s disease (PD) may precede motor manifestations by decades. PD patients’ siblings are at higher risk for PD, but the prevalence and distribution symptoms unknown. The study objectives were (1) to assess non-motor features estimating probability in recruited within European PROPAG-AGEING project; (2) compare well-established DeNoPa cohort. 340 from three sites (Bologna, Seville, Kassel/Goettingen) underwent clinical neurological evaluations markers....
Abstract Next Generation Sequencing (NGS) studies identified 9 functional categories of mutations in acute myeloid leukemia (AML), with >99% cases having at least one those (Ley et al. NEJM 2013). However, multiple genetic hits participate to AML pathogenesis, and metabolic dysregulations, as the induced by IDH1/2 mutations, play oncogenic functions (Ward Cancer Cell 2010). Aim study was define novel affecting relevant druggable biological processes, focus on determinants plasticity....