A5012066076- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Hepatitis B Virus Studies
- T-cell and B-cell Immunology
- Hepatitis C virus research
- Immune Response and Inflammation
- Medical Education and Admissions
- Innovations in Medical Education
- Diversity and Career in Medicine
- RNA Interference and Gene Delivery
- HIV Research and Treatment
- Healthcare professionals’ stress and burnout
- Phagocytosis and Immune Regulation
- vaccines and immunoinformatics approaches
- Liver Disease Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Outbreaks Research
- Immune responses and vaccinations
- Health, psychology, and well-being
- Complement system in diseases
- Orthopedic Surgery and Rehabilitation
- Cytomegalovirus and herpesvirus research
- COVID-19 and Mental Health
- Hepatitis Viruses Studies and Epidemiology
- Educational Strategies and Epistemologies
Erasmus MC
2016-2025
Erasmus University Rotterdam
2006-2023
Leiden University Medical Center
2000-2013
Queen Mary University of London
2011
Leiden University
1998-2005
Robert Koch Institute
2005
Mayo Clinic
2005
University of Bari Aldo Moro
2004
University Medical Center Utrecht
2003
Dendritic cells ( DC s) and monocyte‐derived macrophages (MΦs) are key components of intestinal immunity. However, the lack surface markers differentiating MΦs from s has hampered understanding their respective functions. Here, we demonstrate that, using CD 64 expression, can be distinguished in intestine both mice humans. On that basis, revisit phenotype absence contaminating delineate a developmental pathway healthy leads newly extravasated L y‐6 C hi monocytes to MΦs. We determine how...
Corticosteroids and the calcineurin inhibitors cyclosporin A (CsA) FK506 have been studied extensively regarding their effects on T lymphocytes, but dendritic cells (DC) are relatively unknown. Monocytes one of precursors DC that differentiate into CD14-CD1a+ immature upon culture with IL-4 GM-CSF. The presence CsA or during differentiation did not affect development. In contrast, corticosteroids, either dexamethasone (Dex) prednisolone (Pred), for as little first 48 h blocked generation DC....
Abstract Deficiencies of early components the classical complement pathway, particularly C1q, are strongly associated with susceptibility to systemic lupus erythematosus. Recent data link this predisposal autoimmunity an inappropriate clearance apoptotic cells, which could lead a loss self-tolerance. In present study, we demonstrate that opsonization cells C1q and mannose-binding lectin allows facilitates their uptake not only by macrophages but also human immature dendritic (DCs). Both...
Chronic hepatitis B virus (HBV) infection is the result of an inadequate immune response towards virus. Myeloid dendritic cells (mDC) patients with chronic HBV are impaired in their maturation and function, resulting more tolerogenic rather than immunogenic responses, which may contribute to viral persistence. The mechanism responsible for altered mDC function remains unclear. HBV-infected display large amounts particles proteins circulation, especially surface antigen HBsAg, allows multiple...
Chronic hepatitis B virus (HBV) infection is caused by inadequate anti-viral immunity. Activation of plasmacytoid dendritic cells (pDC) leading to IFNα production important for effective Hepatitis lacks induction in animal models and patients chronic HBV display impaired pDC. Therefore, HBV-derived proteins were examined their effect on human pDC vitro. In addition, the vitro findings compared function derived from ex vivo. contrast other viruses, did not activate Moreover, HBsAg abrogated...
Abstract. Renal allograft rejection is characterized by an influx of inflammatory cells. Interaction between infiltrating T cells and resident parenchymal might play important role in the ongoing response. The present study demonstrates that CD40L, a product activated cells, locally expressed kidneys undergoing rejection. Furthermore, during rejection, CD40 expression not only on most graft but also increased tubular epithelial (TEC). To obtain more detailed insight consequences cell/TEC...
Exhaled NO is increased in patients with asthma and may reflect disease severity. We examined whether the level of exhaled related to degree airway obstruction induced by direct indirect stimuli asthma. Therefore, we measured levels before during recovery from histamine hypertonic saline (HS) challenge (Protocol 1) or histamine, adenosine 5'-monophosphate (AMP), isotonic (IS) 2) 11 nine mild moderate asthma, respectively. The challenges were randomized a 2-d interval. FEV1 at 4, 10, 20, 30...
Abstract The IFN-stimulated response element (ISRE) is an important conserved cis-acting regulatory in the promoter of MHC class I genes, but displays considerable locus-specific nucleotide variation. In this report, putative ISREs classical and nonclassical HLA genes were investigated for their contribution to transactivation. It shown that IFN-γ induced transactivation through ISRE HLA-A, HLA-B, HLA-C, HLA-F. This congruent with binding IFN factor-1 these loci upon treatment. Sp1 was bind...
Background The glycosphingolipid α-galactosylceramide (α-GalCer) is known to stimulate invariant natural killer T-cells (iNKTs) and able induce powerful antiviral immune responses. present dose-escalating randomized placebo-controlled Phase I/II trial aimed investigate activity safety of α-GalCer as a novel class treatment for chronic hepatitis B patients. Methods Patients were randomly assigned 0.1 μg/kg ( n=8), 1 n=6) or 10 placebo n=7) treatment. Results Almost all α-GalCer-treated...
Background. Based on their localization, Kupffer cells (KCs) likely interact with hepatitis B virus (HBV). However, the role of KCs in inducing immunity toward HBV is poorly understood. Therefore, interaction surface antigen (HBsAg) and KCs, possible functional consequences, were assessed.
Immature dendritic cells (DC) reside in peripheral tissues, where they pick up and process incoming pathogens via scavenger receptors or FcR such as FcgammaR FcepsilonR. At mucosal surfaces, IgA is the main Ig to protect body from pathogens. In addition, DC are present high numbers at these sites. We detected expression of FcalphaR (CD89) on CD14+ population CD34+ progenitor-derived monocyte-derived (MoDC). However, CD89 was strongly decreased upon differentiation monocyte DC. found only...
Deficiency in complement proteins such as C1q is associated with the development of systemic lupus erythematosus (SLE). Here, we show that differentiation dendritic cells (DC) presence (C1qDC) gives rise to CD1a(+)/DC-SIGN(+) high phagocytic capacity and low expression CD80, CD83 CD86. Further, when C1qDC were exposed LPS, a significant reduction production IL-6, TNF-alpha IL-10 occurred limited up-regulation In addition, less responsive activation by CD40L terms IL-12p70 secretion CD86...