A5005405528- Plant Pathogens and Fungal Diseases
- Forest Insect Ecology and Management
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Resistance
- Cocoa and Sweet Potato Agronomy
- Plant Virus Research Studies
- Mycorrhizal Fungi and Plant Interactions
- Forest Ecology and Biodiversity Studies
- Legume Nitrogen Fixing Symbiosis
- Plant and Fungal Interactions Research
- Plant and animal studies
- Insect Pest Control Strategies
- Entomopathogenic Microorganisms in Pest Control
- Coffee research and impacts
- Ecology and Vegetation Dynamics Studies
- Postharvest Quality and Shelf Life Management
- Nematode management and characterization studies
- Insect symbiosis and bacterial influences
- Banana Cultivation and Research
- Plant Disease Resistance and Genetics
- Plant and Fungal Species Descriptions
- Algal biology and biofuel production
- Nephrotoxicity and Medicinal Plants
Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center
2015-2025
Indian Institute of Technology Guwahati
2024
Weatherford College
2024
Universidade Federal de Viçosa
2024
Agricultural Research Service
2015-2024
United States Department of Agriculture
2005-2024
University of Hawaiʻi at Mānoa
2011-2018
University of Hawaii at Hilo
2011
University of Hawaii System
2011
Oklahoma State University
1999-2006
The native 'ōhi'a lehua (Metrosideros polymorpha) has cultural, biological and ecological significance to Hawai'i, but it is seriously threatened by a disease commonly referred as rapid death (ROD). Preliminary investigations showed that Ceratocystis species similar C. fimbriata s.lat. was the cause of disease. In this study, we used combination phylogenetic, morphological concepts, well pathogenicity tests microsatellite analyses, characterise isolates collected from diseased trees across...
Guava is one of the most widely grown plants in tropics; however, it affected by many fruit rot diseases. Fruit diseases decrease marketability fresh and for processing. A survey scab disease was conducted at USDA/ARS Tropical Plant Genetic Resource Management Unit Hilo, HI, where more than 50 accessions guava are grown. Symptoms observed were gray/light brown lesions surrounded dark borders on leaves brown, raised, corky, necrotic exocarp which progressed as fruits matured. Seventeen...
Blueberry (Vaccinium spp.) has great economic importance in Chile, which currently about 8,500 ha being cultivated. Recently, the presence of canker and dieback symptoms been observed along productive blueberry zone Chile. Species Pestalotiopsis Truncatella were consistently isolated from diseased samples 22 different locations. Therefore, objective this study was to identify characterize species associated with twig on blueberry. Forty-nine isolates obtained acidified potato dextrose agar...
Distribution Endemic to the six largest Hawaiian islands.Size Greatly variable, typically reaches 20-24 m (66-79 ft) in height; may be much smaller on lava rock or boggy soils.Habitat Wide range, 1-2500 (1-8200 elevation with rainfall of 400-10,000 mm (16-400 in).Largest component lowland and montane wet mesic forests, dry subalpine shrublands, new flows.Vegetation Associated dozens other native species.Soils Very widely adapted, occurring medium heavy clay uplands; tolerates rocky soils...
ABSTRACT Pseudomonas aeruginosa and the phytopathogen P. syringae produce exopolysaccharide alginate, which is a copolymer of d -mannuronic l -guluronic acids. One key regulatory genes controlling alginate biosynthesis in algT , encodes alternate sigma factor, ς 22 . In present study, gene product from pv. showed 90% amino acid identity with its counterpart, sequence analysis region flanking revealed presence nadB mucA mucB an arrangement virtually identical to that An mutant was defective...
Albizia biomass is a forestry waste that has recently been studied for biogas production by solid-state anaerobic digestion (SS-AD). However, albizia chips showed low methane yields because of their recalcitrant structure. In this study, were pretreated Ceriporiopsis subvermispora, white-rot fungus, enhanced SS-AD. After 48 days fungal pretreatment, degradation lignin in was found to be around 24%, which about 2 times cellulose and hemicellulose. Fungal pretreatment resulted more than 4-fold...
Hawaii has long been one of the last coffee-producing regions world free coffee leaf rust (CLR) disease, which is caused by biotrophic fungus Hemileia vastatrix. However, CLR was detected in farms and feral on island Maui February 2020 subsequently other islands Hawaiian archipelago. The source outbreak not known, could have entered from more than 50 nations that harbor pathogen. To determine source(s) inoculum, we analyzed a set eleven simple sequence repeat markers (SSRs) generated...
Pathogenic invasions are a major source of change in both agricultural and natural ecosystems. In forests, fungal pathogens can kill habitat-generating plant species such as canopy trees, but methods for remote detection, mapping monitoring outbreaks poorly developed. Two novel the genus Ceratocystis have spread rapidly across humid mesic forests Hawaiʻi Island, causing widespread mortality keystone endemic tree species, Metrosideros polymorpha (common name: ʻōhiʻa). The process, known Rapid...
Extensive mortality of Metrosideros polymorpha (`ōhi`a) trees has been associated with Ceratocystis spp. on Hawai`i Island and was named rapid `ōhi`a death (ROD). Both C. lukuohia huliohia have ROD, although appears to be the more important pathogen. Crown observations dissections forest either wound-inoculated with, or naturally infected by, were conducted confirm pathogenicity document patterns host colonization. In trials, one three two inoculated fungus in February August, respectively,...
Pseudomonas syringae produces the exopolysaccharide alginate, a copolymer of mannuronic and guluronic acid. Although alginate has been isolated from plants infected by P. syringae, signals timing gene expression in planta have not described. In this study, an algD : uidA transcriptional fusion, designated pDCalgDP, was constructed used to monitor host non-host inoculated with pv. tomato DC3000. When leaves susceptible collard were spray-inoculated DC3000(pDCalgDP), activated within 72 h...
Ceratocystis lukuohia and C. huliohia are recently described fungal species that cause rapid ‘ōhi‘a death (ROD) of Metrosideros polymorpha, Hawaii’s most abundant ecologically important native species. Although the pathogens now widespread on Hawaiʻi Island, a major effort is underway to study manage affected forests, particularly prevent disease from spreading other islands in State or throughout Pacific. Rapid accurate detection critical. Molecular diagnostic real-time PCR protocols were...
Abstract Anthurium amnicola is in the monocot family Araceae, subfamily Pothoideae and a contributing species Hawaii floriculture industry hybrids. To support future genetic improvements to this commodity, we sequenced assembled A. genome chromosome-scale using PacBio HiFi short-read Hi-C sequencing. A total of 98.51% 4.79 Gb anchored into 15 chromosomes, with 99.2% gene completeness high LTR assembly index (LAI) score 21.73, indicative complete, high-quality assembly. Annotation reveals...
Cacao mild mosaic virus (CaMMV), a member of the Badnavirus genus (family: Caulimoviridae) causes an emerging disease on Theobroma cacao. It is associated with branch dieback and has been reported to reduce yield. As there no treatment for infected plants, preventing transmission most effective strategy. However, understand what inoculum reservoirs exist in environment it necessary determine host range virus. Previously, this was only To whether other species could serve as hosts, plants...
Abstract Rapid ʻŌhiʻa Death ( ROD ) is a fungal disease of ʻōhiʻa lehua (Myrtaceae: Metrosideros polymorpha caused by Ceratocystis lukuohia and C. huliohia . the aetiological agent widespread mortality this important tree on Hawaiʻi Island, but its epidemiology remains unclear. We investigated prevalence viability in ambrosia beetle frass ‐affected trees. A total 200 traps were placed onto ‐infected at four locations east side Island. Frass was collected screened for presence DNA using...
Effective forest management, particularly during disturbance events, requires timely and accurate monitoring information at appropriate spatial scales. In Hawai‘i, widespread ‘ōhi‘a (Metrosideros polymorpha Gaud.) mortality associated with introduced fungal pathogens affects stands across the archipelago, further impacting native ecosystems already under threat from invasive species. Here, we share results an integrated program based on high resolution (<5 cm) aerial imagery, field...
During the 2022-2023 season, harvested coffee crop in Hawai'i (
Edible ginger is a popular spice crop that grown in Hawaii primarily for the fresh market, and as such, rhizome quality of paramount importance. In our studies, Gram-negative, facultative anaerobic, rod-shaped bacterium was consistently isolated from decayed well symptomless rhizomes. The identified Enterobacter cloacae by biochemical assays 16S rDNA sequence analysis. Rot symptoms, which usually occurred central cylinder rhizome, were characterized yellowish-brown to brown discolored tissue...
Bacterial diseases of orchids continue to be serious problems. strains were isolated from orchid plants exhibiting disease symptoms in Hawaii. Small large leaf spots with or without water-soaking soft rots observed on various genera, including Dendrobium, Oncidium, and Miltonia spp. hybrids. Bacteria cultured the lesions tentatively identified using analytical profile index (API) strips standard physiological biochemical tests, confirmed by species-specific polymerase chain reaction...
Abstract Anthurium amnicola Dressler possesses a number of desirable and novel ornamental traits such as purple-colored upright spathe, profuse flowering, floral scent, some which have been introgressed into modern cultivars. As first step in identifying genes associated with these traits, the transcriptome from root, leaf, spadix an accession A . was assembled, resulting 28,019 putative transcripts representing 19,458 unigenes. Genes involved pigmentation, including those for metabolism...