A5085543071- biodegradable polymer synthesis and properties
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Insect and Arachnid Ecology and Behavior
- Microplastics and Plastic Pollution
- Carbon dioxide utilization in catalysis
- Insect and Pesticide Research
- Catalysis for Biomass Conversion
- Electrospun Nanofibers in Biomedical Applications
- Anaerobic Digestion and Biogas Production
- Entomopathogenic Microorganisms in Pest Control
- Viral Infectious Diseases and Gene Expression in Insects
- Biochemical and biochemical processes
- Advanced Polymer Synthesis and Characterization
- Microbial Inactivation Methods
- Analytical Chemistry and Sensors
- Microfluidic and Capillary Electrophoresis Applications
- Agriculture Sustainability and Environmental Impact
- Enzyme Catalysis and Immobilization
- thermodynamics and calorimetric analyses
- Insect symbiosis and bacterial influences
- Glycosylation and Glycoproteins Research
- 3D Printing in Biomedical Research
- Microbial bioremediation and biosurfactants
University of Manitoba
2013-2024
National Research Council Canada
2022-2023
A recombinant of Pseudomonas putida LS461 (deletion the phaC1phaZphaC2 genes) was constructed by introducing cosmid JC123 carrying a novel phaC116 gene from metagenomic clone. The resulting strain, P. LS46123, able to synthesize polyhydroxyalkanoate (PHA) polymers with monomer compositions when cultured on glucose or free fatty acids, and accumulated PHAs 9.24% 27.09% cell dry weight. synthesized LS46123 contained up 50 mol % short chain length subunits (3-hydroxybutyrate 3-hydroxyvalerate),...
Medium chain-length polyhydroxyalkanoates (mcl-PHA) were produced by Pseudomonas putida LS46 cultured with a variety of carbohydrate and fatty acid substrates. The monomer compositions molecular weights the polymers varied greatly was dependent on whether substrate metabolized via degradation or de novo synthesis pathways. highest obtained from medium acids, whereas low longer more unsaturated acids carbohydrates. differences in due to choice did not affect polymer thermal point. glass...
Bioplastics represent promising alternatives to petroleum-based plastics, yet their biodegradation remains insufficiently understood. Identifying bacteria capable of degrading bioplastics extracellularly could enhance end-of-life management practices. To investigate Burkholderia's capacities for the degradation medium-chain-length polyhydroxyalkanoate (mcl-PHA), we screened a panel Burkholderia strains and identified such capacity in some B. gladioli, multivorans, vietnamiensis. elucidate...
Clostridium termitidis CT1112 is an anaerobic, Gram-positive, mesophilic, spore-forming, cellulolytic bacterium, originally isolated from the gut of a wood feeding termite Nasusitermes lujae. It has ability to hydrolyze both cellulose and hemicellulose, ferment degradation products acetate, formate, ethanol, lactate, H2, CO2. therefore ges in gene product expression during growth C. on cellobiose, xylose, xylan, α–cellulose. Correlation transcriptome proteome data with fermentation profiles...
A passive embeddable coupled coil sensor for remote bioprocess pH monitoring is described. The sterilizable, able to operate in a fluid medium, and small enough fit inside bioreactor or test tube. It consists of planar spiral inductor connected parallel varactor forming an LC resonant circuit. combination electrode made iridium/iridium oxide sensing silver/silver chloride reference the varactor. potential difference change across electrodes due variation medium changes voltage-dependent...
High cell density (HCD) fed-batch cultures are widely perceived as a requisite for high-productivity polyhydroxyalkanoate (PHA) cultivation processes. In this work, reactive pulse feed strategy (based on real-time CO2 or dissolved oxygen (DO) measurements feedback variables) was used to control an oxygen-limited process improved productivity of medium chain length (mcl-) PHAs synthesized by Pseudomonas putida LS46. Despite the onset limitation half-way through (14 h post inoculation), 28.8 ±...
Glucose and xylose are fermentable sugars readily available from lignocellulosic biomass, a sustainable carbon substrate supporting industrial biotechnology. Three strains were assessed in this work – Paraburkholderia sacchari, Hydrogenophaga pseudoflava, Bacillus megaterium for their ability to uptake both C5 C6 contained hardwood hydrolysate produced via thermomechanical pulping-based process with concomitant production of poly(3-hydroxyalkanoate) (PHA) biopolymers. In batch conditions, B....
A wireless passive pH sensor for continuous remote bioprocess monitoring is presented. The small enough to fit inside a bioreactor or test tube. It consists of planar spiral inductor connected in parallel varactor forming LC resonant circuit. combination electrode made an iridium/iridium oxide sensing and silver/silver chloride reference electrode, the varactor. As medium changes, voltage across electrodes varies, shifting frequency sensor. For in-fluid hermetically sealed encapsulate,...
Abstract Medium chain‐length polyhydroxyalkanoates (mcl‐PHAs) are efficiently synthesized from fatty acids. It was hypothesized that under non‐axenic conditions, the anti‐microbial properties of acids would reduce growth microbial competitors and allow Pseudomonas putida LS46 to dominate population resulting in non‐sterile mcl‐PHA production. Non‐sterile batch fed‐batch cultures produced similar final content, monomer composition, productivity during 24–27 h cultivations when compared...
The rheology of high-cell density (HCD) cultures is an important parameter for its impact on mixing and sparging, process scale-up, downstream unit operations in bioprocess development. In this work, time-dependent rheological properties HCD
<abstract> <bold>Abstract.</bold> Conventional off-line analytical techniques have been used for understanding microbial physiology in many bioprocesses, such as the synthesis of biofuels. Perturbation studies are useful changes metabolism and reaction mechanisms. It is, however, challenging to monitor metabolic pathway perturbations using techniques. This study an on-line âtitrimetric off-gas analysisâ system provide real time information on end-product during fermentation. Clostridium...
<abstract> <b><sc>Abstract.</sc></b> In fermentation processes, it is typically assumed, but seldom justified, that the off-gas composition an adequate indicator of dissolved gas concentrations to which microbes are exposed. The objective this study was measure and assess impact elevated H<sub>2(aq)</sub> CO<sub>2(aq)</sub> (due mass transfer limitations) on carbon balances, electron metabolic flux during thermophlilic with . A membrane inlet spectrometer (MIMS) used for direct on-line...
<abstract> <b>Abstract.</b> Off-gas analysis is a common method for assessing the performance of many bioprocesses, but often little regard given to dissolved gas concentrations which microbes are actually exposed. In this work, membrane-inlet mass spectrometer (MIMS) was used quantitatively assess (H<sub>2</sub> and CO<sub>2</sub>) during thermophilic fermentation 2 g L<sup>-1</sup> cellobiose with Clostridium thermocellum in N<sub>2</sub>-sparged bioreactor. During baseline operating...