A5083366824- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Airway Management and Intubation Techniques
- Neuropeptides and Animal Physiology
- Ion channel regulation and function
- Anesthesia and Sedative Agents
- Cardiac Arrest and Resuscitation
- Cellular transport and secretion
- Nicotinic Acetylcholine Receptors Study
- Ovarian cancer diagnosis and treatment
- Cardiac, Anesthesia and Surgical Outcomes
- Protein Kinase Regulation and GTPase Signaling
- Hereditary Neurological Disorders
- Tracheal and airway disorders
- Emergency and Acute Care Studies
- Respiratory Support and Mechanisms
- BRCA gene mutations in cancer
- Intracerebral and Subarachnoid Hemorrhage Research
- Enzyme function and inhibition
- Pneumothorax, Barotrauma, Emphysema
- Photosynthetic Processes and Mechanisms
- Cassava research and cyanide
- Pharmacological Effects and Assays
- Protein Tyrosine Phosphatases
- Infection Control in Healthcare
Stanford University
2012-2025
Stanford Medicine
2003-2024
Yale University
2017
Medical University of South Carolina
2017
Max Planck Institute of Biochemistry
1998
University of Michigan
1991-1996
Ludwig-Maximilians-Universität München
1996
Mental Health Research Institute
1995
Corning (United States)
1987
Receptor expression enhancing proteins (REEPs) were identified by their ability to enhance cell surface of a subset G protein-coupled receptors (GPCRs), specifically GPCRs that have proven difficult express in heterologous systems. Further analysis revealed they belong the Yip (Ypt-interacting protein) family and some REEP subtypes affect ER structure. comparisons established other potential roles for REEPs, including regulation ER-Golgi transport processing/neuronal localization cargo...
The type I cGMP-dependent protein kinases (cGK Iα and Iβ) form homodimers (subunit Mr ∼ 76,000), presumably through conserved, amino-terminal leucine zipper motifs. Type II kinase II) has been reported to be monomeric (Mr 86,000), but recent cloning sequencing of mouse brain cGK cDNA revealed a motif near its amino terminus. In the present study, recombinant was expressed, purified, characterized. Sucrose gradient centrifugation gel filtration chromatography were used determine values for...
Abstract Background Extended stays in the postanesthesia care unit (PACU) pose challenges high-volume endoscopies. This study investigates impact of intraoperative fentanyl use on PACU duration, postoperative pain, and financial implications outpatient endoscopy. Method A retrospective analysis upper/lower endoscopies at our facility (2020–2022) was conducted, focusing relationship between use, pain scales. Financial impacts were also assessed. Results Among 11,488 patients, 5787 (50.4%)...
Five different splice variants of mouse α2/δ subunit isoforms (α2/a‐e), which arose from various combinations three alternatively spliced regions, were cloned with a combination cDNA library screening and RT‐PCR. Expression patterns relative abundance the in tissues determined an RNAse protection assay. Skeletal muscle brain expressed single isoforms, α2a α2b, respectively; however, cardiovascular system all five isoforms. Heart mainly α2c α2d while, contrast to other species, aorta...
The gamma-aminobutyric acidA (GABA)A receptor (GABAR) beta 1 and gamma 2L subtypes have been shown to be phosphorylated in vitro by protein kinase C (PKC) [J. Biol. Chem. 267:14470-14476 (1992); Neuron 12:1081-1095 (1994)]. To determine the physiological consequences of phosphorylation GABAR isoforms containing subtypes, specific serine residues PKC (beta S409, S327 S343) were changed alanines through site-directed mutagenesis. Wild-type (alpha GABARs) three mutant [alpha 2L(S327A, S343A),...
Although ligand-selective regulation of G protein-coupled receptor-mediated signaling and trafficking are well documented, little is known about whether effects occur on endogenous receptors or such modify the response in physiologically relevant cells. Using a gene targeting approach, we generated knock-in mouse line, which N-terminal hemagglutinin epitope-tagged α2A-adrenergic receptor (AR) expression was driven by α2AAR locus. Exploiting this evaluated α2AAR-mediated inhibition Ca2+...
alpha 1, beta and gamma 2S gamma-aminobutyric acid (GABA) type A receptor (GABAR) subunit cDNAs were transiently expressed in derivative cell lines of mouse L929 fibroblasts, which possessed different levels the catalytic cAMP-dependent protein kinase (PKA). These included (intermediate kinase), C 12 (elevated RAB10 (low kinase) cells. Pharmacological analysis GABA-evoked whole-cell currents revealed that, compared with expression cells, 1 GABARs cells produced a selective enhancement single...
Biochemical, pharmacological and electrophysiological techniques have been used to characterize GABAA receptor (GABAR) channels in mammalian brain demonstrated the existence of a population similar, yet distinct, isoforms. Application molecular biological has revealed multiplicity GABAR subunits subunit subtypes that must assemble form different functional Additionally, sequencing numerous genes for structural motifs which are potentially important regulation. This review summarizes recent...
The number of dual-specificity protein tyrosine phosphatases has grown considerably in the last few years, and thus it would be helpful to organize these novel enzymes. simple term "VHl-like" or "dual-specificity" phosphatase does not adequately reflect different subclasses within this new important subfamily. In article, we review salient features propose a classification scheme enzymes based on their structural motifs. Classification motifs should serve as useful organizational framework...
Single-channel recordings from excised outside-out patches were obtained Chinese hamster ovary cells stably transfected with plasmids containing bovine gamma-aminobutyric acid (GABA) type A (GABAA) receptor channel alpha 1 and beta subunit cDNAs. The predominant or main conductance level recorded had a 17-pS chord conductance. There minor contributions made 25-pS 11-pS levels. Average open duration, burst openings/burst did not change as the GABA concentration was increased 5 to 25 microM....
Receptor expression enhancing proteins (REEPs) were identified by their ability to enhance cell surface of a subset G protein-coupled receptors (GPCRs), specifically GPCRs that have proven difficult express in heterologous systems.Further analysis revealed they belong the Yip (Ypt-interacting protein) family and some REEP subtypes affect ER structure.Yip comparisons established other potential roles for REEPs, including regulation ER-Golgi transport processing/neuronal localization cargo...
This study seeks to evaluate the impact of breast cancer (BRCA) gene status on tumor dissemination pattern, surgical outcome and survival in a multicenter cohort paired primary ovarian (pOC) recurrent (rOC).
Clevidipine-induced pulmonary shunting is a little-reported adverse effect, manifesting as refractory hypoxemia, which may cause significant patient harm. We present the case of mechanically ventilated admitted to intensive care unit following neurosurgical procedure. He was treated postoperatively with clevidipine for blood pressure management, and within 16 hours, he developed profound requiring increased ventilatory support. A workup other causes negative. The hypoxemia recovered 1 hour...