A5080072437- Erythrocyte Function and Pathophysiology
- Cancer-related Molecular Pathways
- Cellular transport and secretion
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Calcium signaling and nucleotide metabolism
University of California, San Diego
2022-2025
La Jolla Bioengineering Institute
2022
AMP-activated protein kinase (AMPK) is a master regulator of cellular energetics which coordinates metabolism by phosphorylating plethora substrates throughout the cell. But how AMPK activity regulated at different subcellular locations for precise spatiotemporal control over unclear. Here we present sensitive, single-fluorophore reporter (ExRai AMPKAR), reveals distinct kinetic profiles mitochondria, lysosome, and cytoplasm. Genetic deletion canonical upstream liver B1 (LKB1) results in...
3-Phosphoinositides (3-PIs), phosphatidylinositol (3,4) bisphosphate [PI(3,4)P 2 ] and (3,4,5) trisphosphate (PIP 3 ), are important lipid second messengers in the Phosphoinositide 3-Kinase (PI3K)/Akt signaling pathway, which is crucial to cell growth frequently dysregulated cancer. Emerging evidence suggests these may be present membranes beyond plasma membrane, yet their spatial regulation within other membrane compartments not well understood. To dissect of specific 3-PI species, we...