Raman spectroscopy is a vibrational spectroscopic technique that can be used to optically probe the molecular changes associated with diseased tissues. The objective of our study was explore near-infrared (NIR) for distinguishing tumor from normal bronchial tissue. Bronchial tissue specimens (12 normal, 10 squamous cell carcinoma (SCC) and 6 adenocarcinoma) were obtained patients known or suspected malignancies lung. A rapid-acquisition dispersive-type NIR system studies at 785 nm...

Neosalanx taihuensis (N. taihuensis) is an important freshwater fish species, rich in nutrients and proteins. However, myofibrillar proteins (MPs), as the major component of muscle, are prone to oxidative denaturation. Tea polyphenols (TPs), natural antioxidants, have broad applications area aquatic product manufacturing. The research examined impacts three typical catechins, namely epicatechin (EC), epigallocatechin (EGC), gallate (EGCG), on structure gel characteristics MP from N. under...

Raman spectroscopy is a molecular vibrational spectroscopic technique that capable of optically probing the biomolecular changes associated with diseased transformation. The purpose this study was to explore near-infrared (NIR) for identifying dysplasia from normal gastric mucosa tissue. A rapid-acquisition dispersive-type NIR system utilised tissue measurements at 785 nm laser excitation. total 76 samples obtained 44 patients who underwent endoscopy investigation or gastrectomy operation...

We successfully acquire the in vivo Raman spectrum of melanin from human skin using a rapid near-infrared (NIR) spectrometer. The signals cutaneous are similar to those observed natural and synthetic eumelanins. is dominated by two intense broad peaks at about 1580 1380 cm–1, which can be interpreted as originating in-plane stretching aromatic rings linear C-C bonds within rings, along with some contributions C-H vibrations methyl methylene groups. Variations peak frequencies bandwidths...

A rapid dispersive-type near-infrared (NIR) Raman spectroscopy system and a probe were developed to facilitate real-time, noninvasive, in vivo human skin measurements. Spectrograph image aberration was corrected by parabolic-line fiber array, permitting complete CCD vertical binning, thereby yielding 3.3-16-fold improvement signal-to-noise ratio. Good quality cutaneous NIR spectra free of interference from fluorescence silica scattering can be acquired less than 1 s, which greatly...

The ability of combining near-infrared (NIR) Raman spectroscopy with support vector machines (SVM) for improving multi-class classification between different histopathological groups in tissues was evaluated this study. A total 105 colonic tissue specimens from 59 patients including 41 normal, 18 hyperplastic polyps and 46 adenocarcinomas were used purpose. rapid-acquisition dispersive-type NIR system utilized spectroscopic measurements at 785-nm laser excitation. 817 spectra acquired...

The aim of this work was to evaluate the biochemical foundation and clinical merit multimodal image-guided Raman endoscopy technique for real-time in vivo diagnosis cancer esophagus during endoscopic examinations. A novel fiber-optic system utilized esophageal measurements at 785 nm laser excitation within 0.5 second under wide-field imaging (white light reflectance (WLR) imaging, narrow-band (NBI) autofluorescence (AFI) guidance. total 75 tissue sites from 27 patients were measured, which...

Optical spectroscopic techniques including reflectance, fluorescence and Raman spectroscopy have shown promising potential for in vivo precancer cancer diagnostics a variety of organs. However, data-analysis has mostly been limited to post-processing off-line algorithm development. In this work, we develop fully automated on-line spectral framework integrated with multimodal image-guided technique real-time detection at endoscopy. A total 2748 gastric tissue spectra (2465 normal 283 cancer)...

Raman spectroscopy is a vibrational spectroscopic technique capable of nondestructively probing endogenous biomolecules and their changes associated with dysplastic transformation in the tissue. The main objectives this study are (i) to develop simultaneous fingerprint (FP) high-wavenumber (HW) confocal (ii) investigate its diagnostic utility for improving vivo diagnosis cervical precancer (dysplasia). We have successfully developed an integrated FP/HW system ball-lens probe acquistion...

Strong field enhancement and confinement in plasmonic nanostructures provide suitable conditions for nonlinear optics ultracompact dimensions. Despite these enhancements, second-harmonic generation (SHG) is still inefficient due to the centrosymmetric crystal structure of bulk metals used, e.g., Au Ag. Taking advantage symmetry breaking at metal surface, one could greatly enhance SHG by engineering surfaces regions where strong electric fields are localized. Here, we combine top-down...

Colorectal cancer can be prevented if detected early (e.g., precancerous polyps‐adenoma). Endoscopic differential diagnosis of hyperplastic polyps (that have little or no risk malignant transformation) and adenomas prominent latency) remains an unambiguous clinical challenge. Raman spectroscopy is optical vibrational technique capable probing biomolecular changes tissue associated with neoplastic transformation. This work aims to apply a fiber‐optic simultaneous fingerprint (FP) high...

Raman spectroscopy (RS), which can detect molecular changes associated with cancer, was explored as a means of distinguishing normal and cancerous nasopharyngeal tissue.Tissue from six patients biopsies studied using rapid acquisition spectrometer.Spectra were obtainable within 5 seconds. Consistent differences noted between cancer tissue in three bands 1,290-1,320 cm(-1) (P = 0.005), 1,420-1,470 0.006), 1,530-1,580 0.002).Spectral appear to exist tissue. The ability obtain spectra rapidly...

Raman spectroscopy is a vibrational spectroscopic technique capable of optically probing the biomolecular changes associated with neoplastic transformation. The purpose this study was to apply near-infrared (NIR) in high wavenumber (HW) region (2800-3700 cm(-1)) for vivo detection cervical dysplasia. A rapid-acquisition NIR system ball-lens fiber-optic probe developed measurements at 785 nm excitation. total 92 HW spectra (46 normal, 46 dysplasia) were acquired from patients Pap smear...

The diagnostic ability of optical spectroscopy techniques, including near-infrared (NIR) Raman spectroscopy, NIR autofluorescence and the composite for in vivo detection malignant tumors was evaluated this study. A murine tumor model, which BALB/c mice were implanted with Meth-A fibrosarcoma cells into subcutaneous region lower back, used purpose. rapid-acquisition dispersive-type system employed tissue spectroscopic measurements at 785-nm laser excitation. High-quality spectra associated an...

We report an integrated Raman spectroscopy and trimodal (white-light reflectance, autofluorescence, narrow-band) imaging techniques for real-time in vivo tissue measurements at endoscopy. A special 1.8 mm endoscopic probe with filtering modules is developed, permitting effective elimination of interference fluorescence background silica fibers while maximizing collections. demonstrate that high-quality spectra upper gastrointestinal tract can be acquired within 1 s or subseconds under the...

Under ultraviolet and visible light excitation, melanin is essentially a nonfluorescent substance. This work reports our study on near-infrared (NIR) fluorescence properties of melanins, explores potential applications NIR techniques for evaluating skin disorders involving melanin. The spectrum obtained using fiber optic spectrometer under 785-nm laser excitation. In vitro measurements are performed synthetic dihydroxyphenylalanine (DOPA) melanin, extracted from Sepia ink sacs, human hair,...

Raman spectroscopy is an optical vibrational technology capable of probing biomolecular changes tissue associated with cancer transformation. This study aimed to characterize in vivo spectroscopic properties tissues belonging different anatomical regions the upper gastrointestinal (GI) tract and explore implications for early detection neoplastic lesions during clinical gastroscopy. A novel fiber-optic endoscopy technique was utilized real-time measurements normal esophageal (distal, middle,...

This study aims to evaluate the clinical utility of image-guided Raman endoscopy for in vivo diagnosis neoplastic lesions stomach at gastroscopy. A rapid-acquisition system with 785-nm excitation has been developed acquire gastric tissue spectra within 0.5 sec during gastroscopic examinations. total 1,063 were acquired from 238 sites 67 patients, which 934 normal whereas 129 tissue. The swarm intelligence-based algorithm (i.e., ant colony optimization (ACO) integrated linear discriminant...

Abstract Intestinal‐type gastric carcinogenesis is a complex multi‐step disease, and early precursors (e.g. intestinal metaplasia (IM), dysplasia) can be very challenging to identify using conventional white‐light endoscopic imaging. This study aims assess the capability of Raman spectroscopy for multi‐class elucidation intestinal‐type sequence in vivo improving precancer detection at endoscopy. We employ novel image‐guided endoscopy technique developed tissue measurement within 0.5 s during...

The aim of this study was to evaluate the feasibility using near-infrared (NIR) Raman spectroscopy for early diagnosis and typing intestinal diffuse adenocarcinoma stomach.A dispersive-type NIR system used tissue measurements. One hundred gastric samples from 62 patients who underwent endoscopy or gastrectomy were (70 normal specimens 30 adenocarcinomas). Principal components analysis (PCA) multinomial logistic regression (MNLR) develop diagnostic algorithms classification.High-quality...

We evaluate the feasibility of applying polarized Raman spectroscopy in probing early biochemical compositions and orientation changes impacted porcine cartilage explants. divide 100 fresh tibial explants into four groups: control (unimpacted) 3 groups single impact at 15, 20, 25 MPa. Each group is examined for using microscopy, cell viability confocal fluorescence histological modified Mankin score. For 15-MPa group, score (p>0.05, n=15) test n=5) reveal no significant when compared to...