A5055183789- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Lipid metabolism and biosynthesis
- Plant biochemistry and biosynthesis
- Fungal and yeast genetics research
- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Microbial Natural Products and Biosynthesis
- Photosynthetic Processes and Mechanisms
- Forest Ecology and Biodiversity Studies
- Biochemical Acid Research Studies
- Bacterial Genetics and Biotechnology
- Bioeconomy and Sustainability Development
- Genomics and Phylogenetic Studies
Acies Bio (Slovenia)
2020-2024
University of Graz
2015-2018
University of Ljubljana
2016
BioTechMed-Graz
2015
Yarrowia lipolytica is a non-conventional yeast that extensively investigated for its ability to excrete citrate or accumulate large amounts of storage lipids, which great significance single cell oil production. Both traits are thus interest basic research as well biotechnological applications but they typically occur simultaneously lowering the respective yields. Therefore, engineering strains with high lipid content relies on novel concepts such computational simulation better understand...
Triacylglycerol (TAG) and glycogen are the two major metabolites for carbon storage in most eukaryotic organisms. We investigated metabolism of oleaginous Yarrowia lipolytica found that this yeast accumulates up to 16% its biomass. Assuming elimination synthesis would result an improvement lipid accumulation, we characterized deleted single gene coding synthase, YlGSY1. The mutant was grown under lipogenic conditions with glucose glycerol as substrates obtained 60% TAG accumulation compared...
Oleaginous microorganisms are characterized by their ability to store high amounts of triacylglycerol (TAG) in intracellular lipid droplets (LDs). In this work, we a protein the oleaginous yeast Yarrowia lipolytica that is associated with LD and plays role regulation TAG storage. This required for phenotype Y. because deletion coding gene results strongly reduced content mutant. Therefore, named it Oleaginicity Inducing protein, Oil1. Furthermore, mutant overexpressing OIL1 accumulates more...
Zeaxanthin, a vital dietary carotenoid, is naturally synthesized by plants, microalgae, and certain microorganisms. Large-scale zeaxanthin production can be achieved through plant extraction, chemical synthesis, or microbial fermentation. The environmental health implications of the first two methods have made fermentation an appealing alternative for natural despite challenges in scaling up bioprocess. An intermediate between β-carotene zeaxanthin, β-cryptoxanthin, found only specific...
Abstract Yarrowia lipolytica is an oleaginous yeast with ever growing popularity in the metabolic engineering circles. It well known for its ability to accommodate a high carbon flux through acetyl-CoA and being extensively studied production of chemicals derived from it. We investigated effects modifying upstream metabolism leading on beta-carotene production, including titer, yield, content. examined pyruvate phosphoketolase bypass, both which are stoichiometrically favorable...