A5026527299- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- 3D Printing in Biomedical Research
- CAR-T cell therapy research
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Advanced Fluorescence Microscopy Techniques
- Cancer Immunotherapy and Biomarkers
- Adenosine and Purinergic Signaling
- Nanoplatforms for cancer theranostics
- T-cell and B-cell Immunology
- ATP Synthase and ATPases Research
- Microtubule and mitosis dynamics
- Protease and Inhibitor Mechanisms
- Photoacoustic and Ultrasonic Imaging
- Angiogenesis and VEGF in Cancer
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- Viral Infectious Diseases and Gene Expression in Insects
- Cell Image Analysis Techniques
- Mitochondrial Function and Pathology
- Medical Imaging Techniques and Applications
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
Radboud University Nijmegen
2016-2025
Radboud University Medical Center
2016-2025
University Medical Center
2016-2025
The University of Texas MD Anderson Cancer Center
2015-2024
Radboud Institute for Molecular Life Sciences
2014-2023
Cancer Genomics Centre
2014-2023
Utrecht University
2022
University of Würzburg
2006-2020
University Medical Center Utrecht
2014-2019
Spanish Oncology Genitourinary Group
2004-2019
Invasive tumor dissemination in vitro and vivo involves the proteolytic degradation of ECM barriers. This process, however, is only incompletely attenuated by protease inhibitor–based treatment, suggesting existence migratory compensation strategies. In three-dimensional collagen matrices, spindle-shaped proteolytically potent HT-1080 fibrosarcoma MDA-MB-231 carcinoma cells exhibited a constitutive mesenchymal-type movement including coclustering β1 integrins MT1–matrix metalloproteinase...
Cell migration through 3D tissue depends on a physicochemical balance between cell deformability and physical constraints. Migration rates are further governed by the capacity to degrade ECM proteolytic enzymes, particularly matrix metalloproteinases (MMPs), integrin- actomyosin-mediated mechanocoupling. Yet, how these parameters cooperate when space is confined remains unclear. Using MMP-degradable collagen lattices or nondegradable substrates of varying porosity, we quantitatively identify...
Repairing tears in the nuclear envelope The segregates genomic DNA from cytoplasm and regulates protein trafficking between cytosol nucleus. Maintaining integrity during interphase is considered crucial. However, Raab et al. Denais show that migrating immune cancer cells experience frequent transitory ruptures when they move through tight spaces (see Perspective by Burke). reseals rapidly interphase, assisted components of ESCRT III membrane-remodeling machinery. Science , this issue pp. 359...
I can see clearly now: Bicyclo[6.1.0]non-4-yne, an easily prepared, symmetrical cycloalkyne, displays excellent reaction kinetics in strain-promoted cycloaddition reactions with azides and nitrones (see scheme). Highly specific protein modifications are demonstrated vitro subcellular-resolved imaging of glycan expression was achieved metastatic melanoma cells during invasive migration into three-dimensional collagen lattices.
Cancer cell invasion is an adaptive process based on cell-intrinsic properties to migrate individually or collectively, and their adaptation encountered tissue structure acting as barrier providing guidance. Whereas molecular physical mechanisms of cancer are well-studied in 3D vitro models, topographic relevance, classification validation toward interstitial organization vivo remain incomplete. Using combined intravital third second harmonic generation (THG, SHG), three-channel fluorescence...