A5087618256- Plant Stress Responses and Tolerance
- Receptor Mechanisms and Signaling
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Neurobiology and Insect Physiology Research
- RNA and protein synthesis mechanisms
- Amino Acid Enzymes and Metabolism
- Photosynthetic Processes and Mechanisms
- Epigenetics and DNA Methylation
- Toxoplasma gondii Research Studies
- ATP Synthase and ATPases Research
- Cancer-related Molecular Pathways
- Adenosine and Purinergic Signaling
- Career Development and Diversity
- Plant responses to elevated CO2
- Evolution and Genetic Dynamics
- Advanced Proteomics Techniques and Applications
- Microtubule and mitosis dynamics
- Erythrocyte Function and Pathophysiology
- Hedgehog Signaling Pathway Studies
- Cell death mechanisms and regulation
- Gene expression and cancer classification
- Cell Adhesion Molecules Research
- Hematopoietic Stem Cell Transplantation
- Plant Water Relations and Carbon Dynamics
Michigan State University
2023-2024
Michigan United
2024
Washington University in St. Louis
2021-2022
Northwestern University
2015-2019
Directed evolution of membrane receptors is challenging as the evolved receptor must not only accommodate a non-native ligand, but also maintain ability to transduce detection new ligand any associated intracellular components. The G-protein coupled (GPCR) superfamily largest group receptors. As members GPCR family detect wide range ligands, GPCRs are an incredibly useful starting point for directed user-defined analytical tools and diagnostics. aim this study was determine if yeast Ste2p...
Rising temperatures due to the current climate crisis will soon have devastating impacts on crop performance and resilience. In particular, CO2 assimilation is dramatically limited at high temperatures. accomplished by rubisco, which inhibited binding of inhibitory sugar phosphates its active site. Plants therefore utilize essential chaperone rubisco activase (RCA) remove these inhibitors enable continued fixation. However, RCA does not function moderately (42oC), resulting in impaired...
The promiscuity of G-protein-coupled receptors (GPCRs) has broad implications in disease, pharmacology and biosensing. Promiscuity is a particularly crucial consideration for protein engineering, where the ability to modulate model essential developing desirable proteins. Here, we present methodologies (i) modifying GPCR using directed evolution (ii) predicting receptor response identifying important peptide features quantitative structure-activity relationship models grouping-exhaustive...
During the COVID-19 pandemic, existing Student Inquiry and Research program at Illinois Mathematics Science Academy transitioned to a remote-only format. I describe my experience of mentoring 12 high school juniors seniors in yearlong remote research projects involving plant biology protein engineering. Working groups two or three, students read scientific articles proposed experiments. An experienced researcher then carried out experiments gave data. Students analyzed data, drew conclusions...
Rising temperatures due to the current climate crisis will have devastating impacts on crop performance and resilience in near future. One key step that limits plant photosynthetic under higher is activity of thermolabile enzyme rubisco activase (RCA). RCA highly conserved organisms, including C4 crops such as Zea mays (maize) Sorghum bicolor (sorghum) which are crucial components global food supply bioenergy sector. While most abundant protein earth responsible for carbon fixation, an...
P-type ATPase are ubiquitous transport proteins across all kingdoms of life. These share a common mechanism involving phosphorylation an invariant aspartate to facilitate movement substrates from protons phospholipids cellular membranes. In this issue the Biochemical Journal, Welle et al. identify conserved cysteine near functionally critical plasma membrane H+-ATPases that protects protein reactive oxygen species.
Abstract C 4 crops such as Zea mays (maize) and Sorghum bicolor (sorghum) are crucial producers of food bioenergy. In these crops, carbon assimilation is limited at higher temperatures because the enzyme rubisco activase (RCA), which responsible for proper function CO 2 fixing protein rubisco, has been shown to be thermolabile. We present a holistic approach understand RCA in maize, sorghum, model grass Setaria viridis (setaria) during heat stress acclimation. Gas exchange data confirms that...
C crops such as Zea mays (maize) and Sorghum bicolor (sorghum) are important in both food bioenergy systems. In these crops, carbon assimilation is limited at high heat by the thermolabile protein rubisco activase (RCA). We present a comparative study of RCA function grasses maize, sorghum, model grass Setaria viridis (setaria) during 48 hour stress acclimation. Western blots biochemical assays show that each species uses different strategy to acclimate heat. All shift predominant ꞵ...