A5050347786- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Transgenic Plants and Applications
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Nanoplatforms for cancer theranostics
- Advanced Biosensing Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Respiratory viral infections research
- Virology and Viral Diseases
- Biosensors and Analytical Detection
- Veterinary Oncology Research
- Lanthanide and Transition Metal Complexes
- SARS-CoV-2 detection and testing
- Cancer Research and Treatments
- Molecular Junctions and Nanostructures
- Protein purification and stability
- Microfluidic and Bio-sensing Technologies
- Immune Cell Function and Interaction
University of California, San Diego
2010-2025
Moores Cancer Center
2018-2025
La Jolla Bioengineering Institute
2019-2025
University of California System
2022-2025
University of California San Diego Medical Center
2023
Case Western Reserve University
2013-2022
University School
2012-2022
Dartmouth–Hitchcock Medical Center
2022
UC San Diego Health System
2020-2021
Bioengineering Center
2021
The copper-catalyzed azide−alkyne cycloaddition (CuAAC) reaction, optimized for biological molecules in aqueous buffers, has been shown to rapidly label mammalian cells culture with no loss cell viability. Metabolic uptake and display of the azide derivative N-acetylmannosamine developed by Bertozzi, followed CuAAC ligation using sodium ascorbate ligand tris(hydroxypropyltriazolyl)methylamine (THPTA), gave rise abundant covalent attachment dye−alkyne reactants. THPTA serves both accelerate...
The use of microneedles has facilitated the painless localized delivery drugs across skin. However, their efficacy been limited by slow diffusion molecules and often requires external triggers. Herein, an autonomous degradable, active microneedle platform is introduced, employing magnesium microparticles loaded within patch, as built-in engine for deeper faster intradermal payload delivery. particles react with interstitial fluid, leading to explosive-like rapid production H2 bubbles,...
The size and shape of nanocarriers can affect their fate in vivo, but little is known about the effect nanocarrier aspect ratio on biodistribution setting cancer imaging drug delivery. production nanoscale anisotropic materials a technical challenge. A unique biotemplating approach based rod‐shaped nucleoprotein nanoparticles with predetermined ratios (AR 3.5, 7, 16.5) used. These rigid, soft‐matter nanoassemblies are derived from tobacco mosaic virus (TMV) components. role nanoparticle...
Phenanthriplatin, cis-[Pt(NH3)2Cl(phenanthridine)](NO3), is a cationic monofunctional DNA-binding platinum(II) anticancer drug candidate with unusual potency and cellular response profiles. Its in vivo efficacy has not yet been demonstrated, highlighting the need for delivery system. Here we report tobacco mosaic virus (TMV) as system phenanthriplatin. TMV forms hollow nanotubes polyanionic interior surface; capitalizing on this native structure, developed one-step phenanthriplatin loading...
The underlying cause of major cardiovascular events, such as myocardial infarctions and strokes, is atherosclerosis. For accurate diagnosis this inflammatory disease, molecular imaging required. Toward goal, we sought to develop a nanoparticle-based, high aspect ratio, molecularly targeted magnetic resonance (MR) contrast agent. Specifically, engineered the plant viral nanoparticle platform tobacco mosaic virus (TMV) target vascular cell adhesion molecule (VCAM)-1, which highly expressed on...
Multivalent nanoparticle platforms are attractive for biomedical applications because of their improved target specificity, sensitivity, and solubility. However, controlled assembly remains a considerable challenge. An efficient hydrazone ligation chemistry was applied to the Cowpea mosaic virus (CPMV) nanoparticles with individually tunable levels VEGFR-1 ligand fluorescent PEGylated peptide. The recognized on endothelial cell lines VEGFR1-expressing tumor xenografts in mice, validating...
Nanomaterials with elongated architectures have been shown to possess differential tumor homing properties compared their spherical counterparts. Here, we investigate whether this phenomenon is mirrored by plant viral nanoparticles that are filamentous (Potato virus X) or (Cowpea mosaic virus). Our studies demonstrate Potato X (PVX) and Cowpea (CPMV) show distinct biodistribution profiles differ in penetration efficiency. Analogous what seen inorganic nanomaterials, PVX shows enhanced tissue...
Quartz crystal microbalance (QCM) is widely used for studying soft interfaces in liquid environment. Many of these are heterogeneous nature, the sense that they composed discrete, isolated entities adsorbed at a surface. When characterizing such interfaces, one interested determining parameters as surface coverage and size surface-adsorbed entities. The current strategy to obtain this information by fitting QCM data—shifts resonance frequency, ΔF, bandwidth, ΔΓ—with model derived smooth,...
The plant virus, Cowpea mosaic virus (CPMV), is developed as a carrier of the chemotherapeutic drug doxorubicin (DOX). CPMV-DOX conjugate, in which eighty DOX molecules are covalently bound to external surface carboxylates viral nanoparticle (VNP), shows greater cytotoxicity than free toward HeLa cells when administered at low dosage. At higher concentrations, time-delayed. CPMV conjugate targeted endolysosomal compartment cells, proteinaceous degraded and released. This study first...
Quartz crystal microbalance with dissipation monitoring (QCM-D) has become a popular tool to investigate biomolecular adsorption phenomena at surfaces. In contrast optical mass-sensitive techniques, which commonly detect the adsorbed nonhydrated mass, mechanically coupled mass measured by QCM-D includes significant amount of water. A mechanistic and quantitative picture how surrounding liquid couples deposited solutes so far been elusive for apparently simple like random nanometer-sized...
PEGylation is an effective strategy for reducing biospecific interactions pharmaceuticals. The plant virus Cowpea mosaic (CPMV) has been studied potential nanobiomedical applications by virtue of its natural with mammalian endothelial cells. To investigate the degree required to retarget CPMV-based formulations other destinations, two CPMV-PEG formulations, CPMV-PEG1000 (P1) and CPMV-PEG2000 (P2) were tested. Modeling suggested that PEG chains displayed as flattened mushrooms on particle...