A5056901131- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Fermentation and Sensory Analysis
- Insect symbiosis and bacterial influences
- Insect Utilization and Effects
- Wastewater Treatment and Nitrogen Removal
- Coastal wetland ecosystem dynamics
- Genomics and Phylogenetic Studies
- Insect and Pesticide Research
- Cassava research and cyanide
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Insect-Plant Interactions and Control
- Biochemical and biochemical processes
- Horticultural and Viticultural Research
- Animal and Plant Science Education
- Agronomic Practices and Intercropping Systems
- Wine Industry and Tourism
- Bacterial biofilms and quorum sensing
- Infections and bacterial resistance
- Yeasts and Rust Fungi Studies
- Banana Cultivation and Research
- Plant Parasitism and Resistance
- Meat and Animal Product Quality
- Listeria monocytogenes in Food Safety
KU Leuven
2015-2025
Ecologie Microbienne Lyon
2018
Janssen (Belgium)
1987-1998
Swarming motility is suggested to be a social phenomenon that enables groups of bacteria coordinately and rapidly move atop solid surfaces. This multicellular behavior, during which the apparently organized bacterial populations are embedded in an extracellular slime layer, has previously been linked with biofilm formation virulence. Many population density-controlled activities involve activation complex signaling pathways using small diffusible molecules, also known as autoinducers. In...
<i>Rhizobium etli</i> CNPAF512 produces an autoinducer that inhibits growth of leguminosarum</i> bv. <i>viciae</i> 248 and activates the<i>Agrobacterium tumefaciens tra</i> reporter system. Production this compound in <i>R. is dependent on two genes, named <i>cinR</i> <i>cinI</i>, postulated to code for a transcriptional regulator synthase, respectively. NMR analysis the purified molecule indicates produced by CinI saturated long chain 3-hydroxy-acyl-homoserine lactone, abbreviated as...
High soil temperatures in tropical areas limit nodulation and dinitrogen fixation by strains of Rhizobium. Several heat-tolerant bean-nodulating Rhizobium have been isolated previously. However, the basis their resistance to heat remains unknown. In this study, we compared effects on symbiotic nitrogen fixation, cell survival, amino acid uptake, protein synthesis a (CIAT899) heat-sensitive (CNPAF512) strain. Acetylene reduction activity nodulated roots excised from unstressed plants was...
In this study, the microbiota during industrial rearing, processing, and storage of edible tropical house cricket, Gryllodessigillatus, was investigated. To end, we analyzed samples from cricket feed, obtained before feeding as well cages, crickets after harvest, processing into frozen, oven-dried, smoked oven-dried (smoked/dried) end products. Although feed contained lower microbial numbers than crickets, both were dominated by same species-level operational taxonomic units, determined...
Autoinduction plays an important role in intercellular communication among symbiotic and pathogenic gram-negative bacteria. We report here that a nitrogen-fixing symbiont of Phaseolus vulgaris, Rhizobium etli CNPAF512, produces at least seven different autoinducer molecules. One them exhibits growth-inhibitory effect like the bacteriocin small [N-(3R-hydroxy-7-cis-tetradecanoyl)-L-homoserine lactone]. At two other autoinducers are synthesized by LuxI-homologous synthase. The corresponding...
Gut microbial communities are critical for the health of many insect species. However, little is known about how gut respond to anthropogenic changes and such affect host-pathogen interactions. In this study, we used deep sequencing investigate compare composition within midgut ileum (both bacteria fungi) in Bombus terrestris queens collected from natural (forest) urbanized habitats. Additionally, investigated whether variation under each habitat affected prevalence two important bumblebee...
Most bacteria recovered so far from sugar thick juice during storage represent strains of the species Tetragenococcus halophilus. Recently, several Gram-positive, non-motile, non-spore-forming cocci with other physiological and genetic traits were isolated samples different origins. In this study, representative isolates investigated using a polyphasic taxonomic approach. The 16S rRNA gene sequence similarity between these their closest relative, muriaticus, was 97.4%. level DNA-DNA...
Brettanomyces (Dekkera) bruxellensis is an ascomycetous yeast of major importance in the food, beverage and biofuel industry. It has been isolated from various man-made ecological niches that are typically characterized by harsh environmental conditions such as wine, beer, soft drink, etc. Recent comparative genomics studies revealed immense intraspecific diversity, but it still unclear whether this genetic diversity also leads to systematic differences fermentation performance (off-)flavor...
Currently, one of the most important challenges is to provide sufficient and affordable food energy for a fast-growing world population, alongside preserving natural habitats maintaining biodiversity. About 35% global production depends on animals pollination. In recent years, an alarming worldwide decline in pollinators has been reported, putting our under additional pressure. Therefore, there urgent need find sustainable ways ensure this crucial ecosystem service. Recent studies have shown...
ABSTRACT The Rhizobium etli rpoN1 gene, encoding the alternative sigma factor ς 54 (RpoN), was recently characterized and shown to be involved in assimilation of several nitrogen carbon sources during free-living aerobic growth (J. Michiels, T. Van Soom, I. D’hooghe, B. Dombrecht, Benhassine, P. de Wilde, J. Vanderleyden, Bacteriol. 180:1729–1740, 1998). We identified a second rpoN gene copy R. , rpoN2 54.0-kDa protein which displays 59% amino acid identity with RpoN1 protein. is...
Rhizobium tropici strains are able to nodulate a wide range of host plants: Phaseolus vulgaris, Leucaena spp., and Macroptilium atropurpureum. We studied the nodD regulatory gene for nodulation two R. strains: CIAT899, reference type IIb strain, BR816, heat-tolerant strain isolated from leucocephala. A survey revealed several nodD-hybridizing DNA regions in both five distinct CIAT899 four BR816. Induction experiments nodABC-uidA fusion combination with different fragments presence root...
Summary In general, oxidative stress, the consequence of an aerobic lifestyle, induces bacterial antioxidant defence enzymes. Here we report on a peroxiredoxin Rhizobium etli , prxS strongly expressed under microaerobic conditions and during symbiotic interaction with Phaseolus vulgaris . The induction prxS‐rpoN 2 operon is mediated by alternative sigma factor RpoN enhancer‐binding protein NifA. RpoN‐dependent promoter also active low‐nitrogen through NtrC. An additional symbiosis‐specific...
ABSTRACT The symbiotic interaction between Rhizobium etli and Phaseolus vulgaris , the common bean plant, ultimately results in formation of nitrogen-fixing nodules. Many aspects intermediate late stages this are still poorly understood. R. relA gene was identified through a genome-wide screening for mutants. RelA has pivotal role cellular physiology, as it catalyzes synthesis (p)ppGpp, which mediates stringent response bacteria. ppGpp abolished an mutant strain under conditions amino acid...
In this study, six commercial meat starters, each consisting of a pure strain lactic acid-fermenting bacterium (including Lactococcus lactis, Lactobacillus curvatus, L. farciminis, plantarum, sakei, and Pediococcus acidilactici), were tested for their ability to ferment paste produced from the yellow mealworm (Tenebrio molitor). During fermentation, microbial counts, pH, bacterial community composition determined. addition, UPLC-MS was applied monitor consumption glucose production glutamic...
Abstract Background The microbiome of many insects consists a diverse community microorganisms that can play critical roles in the functioning and overall health their hosts. Although microbial communities have been studied thoroughly over past decade, little is still known about how biotic interactions affect structure on bodies insects. In are attacked by parasites or parasitoids, it be expected host insect affected presence these parasitic organisms develop close association with host....
M ichiels , K., V erreth C. & anderleyden J. 1990. Azospirillum lipoferum and brasilense surface polysaccharide mutants that are affected in flocculation. Journal of Applied Bacteriology 69 705–711. Surface production by is demonstrated fluorescence colonies grown on media containing the fluorescent dye Calcofluor, which binds to β‐linked polysaccharides. Mutants showing decreased increased levels obtained from strain Sp59b chemical mutagenesis, A. 7030 Tn5 mutagenesis. The produce...