A5082646842- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Response and Inflammation
- Genomics and Chromatin Dynamics
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Tryptophan and brain disorders
- Anesthesia and Neurotoxicity Research
- Neurogenesis and neuroplasticity mechanisms
- Alcohol Consumption and Health Effects
- Microtubule and mitosis dynamics
- Peroxisome Proliferator-Activated Receptors
- DNA Repair Mechanisms
University of North Carolina at Chapel Hill
2017-2021
Alcohol abuse and binge drinking are common during adolescence, a developmental period characterized by heightened neuroplasticity. Animal studies reveal that adolescent ethanol exposure decreases hippocampal neurogenesis persists into adulthood, but the mechanism remains to be fully elucidated. Using rodent model of intermittent (AIE; 5.0 g/kg, i.g., 2-days on/2-days off from postnatal day [P]25 P55), we tested hypothesis AIE-induced upregulation neuroimmune signaling contributes loss in...
Ethanol (EtOH) consumption leads to an increase of proinflammatory signaling via activation Toll-like receptors (TLRs) such as TLR3 and TLR4 that kinase (ERK1/2, p38, TBK1), transcription factor (NFκB, IRF3), increased cytokines TNF-α, IL-1β, IL-6. This immune cascade is thought play a role in neurodegeneration alcohol use disorders. While microglia are considered be the primary macrophage brain, it unclear what if any neurons EtOH-induced signaling.Microglia-like BV2 retinoic...
Abstract The nucleolus is the site of ribosome biosynthesis encompassing ribosomal DNA (rDNA) locus in a phase separated state within nucleus. In budding yeast, we find rDNA and Cdc14, protein phosphatase that co-localizes with rDNA, behave like condensate formed by polymer–polymer separation, while ribonucleoproteins liquid-liquid separation. compaction Cdc14’s nucleolar distribution are dependent on concentration cross-linkers. contrast, ribonucleoprotein independent cross-linkers...
Alcohol use disorder (AUD) involves dysregulation of innate immune signaling in brain. Toll-like receptor 3 (TLR3), an that is upregulated post-mortem human alcoholics, leads to induction interferon (IFN) signaling. IFNs have been linked depressive-like symptoms and therefore may play a role addiction pathology. Astrocyte-neuronal contribute maladaptation neuronal circuits.In this manuscript, we examine ethanol (EtOH) IFN neuronal, astrocyte, microglial cell lines assess astrocyte-neuronal...
Innate immune signaling molecules, such as Toll-like receptors (TLRs), cytokines and transcription factor NFκB, are increased in post-mortem human alcoholic brain may play roles alcohol dependence neurodegeneration. involves microglia -neuronal which while poorly understood, impact learning memory. To investigate mechanisms of ethanol induction innate within between cells, we studied immortalized BV2 SH-SY5Y neuroblastoma to model microglial neuronal signaling. Cells were treated alone or...
DNA double-strand breaks arise in vivo when a dicentric chromosome (two centromeres on one chromosome) goes through mitosis with the two attached to opposite spindle pole bodies. Repair of DSBs generates phenotypic diversity due range monocentric derivative chromosomes that arise. To explore whether may be differentially repaired as function their spatial position chromosome, we have examined structure from cells containing suite which distance between ranges 6.5 kb 57.7 kb. Two major...