A5017126066- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- RNA Research and Splicing
- DNA Repair Mechanisms
- Design Education and Practice
- Fungal and yeast genetics research
- Advanced Optical Imaging Technologies
- Manufacturing Process and Optimization
- 3D Shape Modeling and Analysis
- Muscle Physiology and Disorders
- Computer Graphics and Visualization Techniques
- Virtual Reality Applications and Impacts
- Tactile and Sensory Interactions
- Image Processing and 3D Reconstruction
- Mechatronics Education and Applications
- Educational Robotics and Engineering
- Cellular transport and secretion
- Artificial Intelligence in Games
- Chromosomal and Genetic Variations
- Creativity in Education and Neuroscience
- Product Development and Customization
- Intelligent Tutoring Systems and Adaptive Learning
- Ion channel regulation and function
- Cognitive Science and Mapping
- Innovation and Knowledge Management
HTW Berlin - University of Applied Sciences
2015-2018
Rockefeller University
2007-2017
Friedrich Miescher Institute
2005-2012
Fraunhofer Institute for Telecommunications, Heinrich Hertz Institute
2008-2011
Fraunhofer Society
2009
University of Geneva
2002-2005
University of Basel
2001
We present a new, robust, computational procedure for tracking fluorescent markers in time-lapse microscopy. The algorithm is optimized finding the time-trajectory of single particles very noisy dynamic (two- or three-dimensional) image sequences. It proceeds three steps. First, images are aligned to compensate movement biological structure under investigation. Second, particle's signature enhanced by applying Mexican hat filter, which we show be optimal detector Gaussian-like spot 1/omega2...
A long-standing biological question is how a eukaryotic cell controls the size of its nucleus. We report here that in fission yeast, nuclear proportional to over 35-fold range, and use mutants show 16-fold change DNA content does not influence relative Multi-nucleated cells with unevenly distributed nuclei reveal surrounded by greater volume cytoplasm grow more rapidly. During interphase cycle growth growth, during mitosis there rapid expansion envelope. When nuclear/cell (N/C) ratio...
Chromatin in the interphase nucleus moves a constrained random walk. Despite extensive study, molecular causes of such movement and its impact on DNA-based reactions are unclear. Using high-precision live fluorescence microscopy budding yeast, we quantified tagged chromosomal loci to which transcriptional activators or nucleosome remodeling complexes were targeted. We found that local binding activator VP16, but not Gal4 acidic domain, enhances chromatin mobility. The increase did correlate...
How cells control the overall size and growth of membrane-bound organelles is an important unanswered question cell biology. Fission yeast maintain a nuclear proportional to cellular size, resulting in constant ratio between volumes (N/C ratio). We have conducted genome-wide visual screen fission gene deletion collection for viable mutants altered their N/C ratio, found that defects both nucleocytoplasmic mRNA transport lipid synthesis alter ratio. Perturbing export results accumulation...
Abstract— The development of a multi‐user stereoscopic display that does not require the use special glasses (autostereoscopic), and enables large degree freedom viewer movement requires only minimum amount information (a stereo pair) for displays described. optics comprise an RGB holographic laser projector is controlled by output multi‐target head‐position head tracker, optical assembly converts into steerable exit pupils, screen comprising single liquid‐crystal (LCD) image multiplexing...
The construction and operation of two laser-based glasses-free 3D (autostereoscopic) displays that have been carried out within the European Union-funded projects MUTED HELIUM3D is described in this paper. Both use a multi-user head tracker to direct regions viewer's referred as exit pupils eyes. employs direct-view LCD whose backlight comprises novel steering optics image information supplied by horizontally-scanned fast light valve output controlled spatial modulator (SLM). principle...
The positioning of chromosomal domains within interphase nuclei is thought to contribute establishment and maintenance epigenetic control. Using GFP-tagged domains, LexA-fusion targeting live microscopy, we investigated mechanisms through which chromatin can be anchored the nuclear envelope (NE). We find that a subdomain silencing information regulator Sir4 (Sir4(PAD)) yKu80 are sufficient tether region periphery, independently their function. Sir4(PAD) interacts with Esc1 (Establishes...
Es wird ein neu entwickeltes geometrisches Normal zur Prüfung von Röntgen-Mikro-Computertomografiemesssystemen (μCT-Messsystemen) sowie Verfahren Herstellung und Kalibrierung beschrieben. Das ermöglicht die μCT-Messsystemen mit den der klassischen Koordinatenmesstechnik nach ISO 10360 bzw. VDI/VDE 2617 dem des Soll-Ist-Vergleiches Referenz- μCT-Messdaten.
Abstract— The Multi‐User 3‐D Television Display (MUTED), designed to provide three‐dimensional television (3‐D TV) by the display of autostereoscopic imagery multiple viewers, each whom should enjoy freedom movement, is described. Such an system, which allows viewers simultaneously use head tracking, was previously demonstrated for TV applications in ATTEST project. However, requirement a dynamically addressable, steerable backlight presented several problems illumination source. MUTED...
The development of tracked 3D displays is based on real-time technologies providing precise and robust eye location detection. paper focuses tracking approaches which have been developed within the European HELIUM3D project. A first system uses multiple common video cameras a second approach combining high resolution depth camera technology. Flexible scalable software has supporting such different hardware implementations. Beside descriptions will focus performance evaluations accomplished...
During product development, shape-based similarity search helps the engineering teams to discover parts and assemblies already existing in their company's component databases that match ones required for new product. Therefore, constitutes an important means keep overall number of components designed, managed maintained by a company at lowest possible level. This reducing efforts design, manufacturing planning, managing master data, consequently lowers costs. In this paper, we present...