Novel silver/poly-1-vinyl-1,2,4-triazole nanocomposite materials-possessing antimicrobial activity against Gram-positive and Gram-negative bacteria-have been synthesized characterized in the solid state aqueous solution by complex of modern physical-chemical biologic methods. TEM-monitoring has revealed main stages microbial cell (E. coli) destruction novel nanocomposite. The concept direct polarized microbes nanosilver proposed authors allows relationship between physicochemical properties...

A bright far-red emitting unsymmetrical meso-CF3-BODIPY fluorescent dye with phenyl and pyrazolyl substituents was synthesized by condensation of trifluoropyrrolylethanol pyrazolyl-pyrrole, subsequent oxidation complexation the formed dipyrromethane. This BODIPY exhibits optical absorption at λab ≈ 610-620 nm emission λem 640-650 nm. The studied on Ehrlich carcinoma cells as a lysosome-specific that allows intravital staining cell structures real-time monitoring changes occurring in cells....

In recent decades, the methods based on cell technologies have become more relevant for medical and pharmaceutical research. this paper, weconsidered BioStation CT using continuous in vivo observation of Ehrlich ascitic carcinoma culture under influence two active substances different concentrations (from 1.25 to 20 mg/l). As a result cells condition monitoring 4.5 days it was shown that substance No 2 does not affect viability while 1 causes death, effect is dose-dependent....

и травматологии»

Severe injuries of the musculoskeletal system with damage bone and cartilaginous tissue are associated necessity to find ways for replacement defects. Current methods treatment including usage autografts modern synthetic materials often not effective enough. The article deals prospects developing new areas in traumatological pathology based on cell technologies application. Both medical ethical aspects technology utilizing various sources material transplantation discussed detail. Special...

A new original copper nanocomposite based on poly-N-vinylimidazole was synthesized and characterized by a complex of modern physicochemical biological methods. The low cytotoxicity the in relation to cultured hepatocyte cells found. possibility involve from functioning copper-dependent enzyme systems evaluated during incubation culture with this introduced nutrient medium. water-soluble copper-containing is promising for biotechnological biomedical research as non-toxic hydrophilic...

The review is devoted to the compilation of data on toxicity and safety using nanosized forms selenium. It shown that in nature selenium exists mainly form selenate (Se6+), selenite (Se4+), selenide (Se2–) elemen-tal (Se0), while latter insoluble aqueous media, less toxic biologically inert. Elemental particles not only biocompatible, but also has antitumor antimicrobial activity. was elemental nanoparticles can modulate activity antioxidant detoxification systems. A dose-dependent effect...

Background . Copper ions are necessary for maintaining basic physiological processes in the mammalian organism. However, their excessive absorption or accumulation cells can lead to development exacerbation of various pathological processes. The cytotoxic and genotoxic effects high concentrations copper compounds currently well studied cell cultures, whereas effect non-toxic amounts on cells, including during cultivation, has been extremely poorly studied. aim study To investigate changes...

Background: Mitochondrial oxidative phosphorylation (OxPhos) accounts for more than 90% of the cellular ATP production, plays role in reactive oxygen species (ROS) generation and programmed cell death.In addition, it contributes to such processes as proliferation, differentiation aging.Currently, several signaling systems are known participate regulation OxPhos activity cytochrome c-oxidase (CcO), terminal enzyme mitochondrial electron transport chain.However, data on mechanisms key units...

Background: The aim of this study was to assess the effect blockade p38 mitogen-activated protein kinase (MAPK) on expression genes encoding metalloproteinases (MMPs) during formation adhesions in abdominal cavity. Methods and Results: experiments were carried out male Wistar rats (n=75). studies two groups: Group 1 (control, n=35) – modelling adhesive process; 2 (experimental, process with intraperitoneal administration Seroguard®—a prolonged form MAPK inhibitor. MMP1a, MMP2, MMP7, MMP9,...

The article describes a biochemical method for characterizing the hepatocyte culture under action of CA. Isolated primary was exposed to CA in different concentrations, and then activity ALT, AST, ALP, amount glucose, medium determined. result showed that level cell membrane damage release ALT AST into extracellular space depend on concentration acting substance, and, addition, found be more sensitive toxic effects However, ALP proved an indicator copper ions' enzyme but not destructive...

Hydrogels are a class of dimensional hydrophylic polymer networks capable absorbing and retaining large amounts water. Natural synthetic components can serve as material for the hydrogel production. have unique physico-chemical properties, which determined by composition concentration, its density, crosslinking methods, production approaches. This review article describes natural materials used hydrogels having different properties. The collagen, elastin, gelatin, chitosan, dextran,...

In recent decades, a large number of different nanocomposite materials have appeared, which found wide application in almost all areas life, including medicine.However, to date, the properties these not been completely studied.The evaluation toxicity and biocompatibility is particularly relevant.In this regard, study aimed investigate effect surface treatment method light-cured material on its toxic for normal fibroblasts.For purpose, nanohybrid composite Herculite XRV Ultra form 10×5 mm...

АБДОМИНАЛЬНАЯ ХИРУРГИЯ ■Энтеральная недостаточность оказывает важное влияние на течение острого перитонита; от ее своевременной диагностики и лечения зависит исход заболевания.Цель -разработать способ прогнозирования энтеральной недостаточности при остром перитоните.Материал методы.Исследование выполнено с использованием 18 крыс-самцов породы Wistar в возрасте 6 мес, массой тела 250-300 г.Животным выполняли моделирование послеоперационного разлитого перитонита по разработанному нами...

ИзУЧЕНИЕ КУЛЬТУРы ФИБРОБЛАСТОВ ПОД ВОзДЕЙСТВИЕМ РОСТОВОгО ФАКТОРАТрухан И.С., Дремина Н

Lysosomes are multifunctional cell organelles that not only provide degradation of macromolecules by lumenal acid hydrolases, but also contribute in the regulation metabolism, ion homeostasis maintenance, and programmed death induction. The study this compartment various pathological conditions, including oncological diseases different origins is particular interest. This article discusses lysosome involvement process malignant transformation, as well role these tumor metastasis mediated...

Injuries and degenerative changes of tendons are common damages the musculoskeletal system. Due to its hypovascular character tendon has a limited natural ability recover. For typical surgical treatment, integrity is restored, but in most cases, there occurs formation connective tissue scar resulting structural mechanical functionality disruption. The insufficient effectiveness traditional therapy methods requires search for alternative ways restore damaged tissues. This article discusses...