A5034988192- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Immune Response and Inflammation
- Respiratory viral infections research
- T-cell and Retrovirus Studies
- Probiotics and Fermented Foods
- interferon and immune responses
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Microbial infections and disease research
- Cytomegalovirus and herpesvirus research
- Antimicrobial Peptides and Activities
- Toxin Mechanisms and Immunotoxins
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Milk Quality and Mastitis in Dairy Cows
- Dermatology and Skin Diseases
- Infant Nutrition and Health
- vaccines and immunoinformatics approaches
- Insect symbiosis and bacterial influences
Yangzhou University
2016-2025
ABSTRACT PA-X is a newly discovered protein that decreases the virulence of 1918 H1N1 virus in mouse model. However, role pathogenesis highly pathogenic avian influenza viruses (HPAIV) H5N1 subtype species totally unknown. By generating two PA-X-deficient and evaluating their different animal models, we show here diminishes HPAIV strain A/Chicken/Jiangsu/k0402/2010 (CK10) mice, chickens, ducks. Expression dampens polymerase activity replication both vitro vivo . Using microarray analysis,...
African swine fever (ASF) caused by the virus (ASFV) is a fatal and highly contagious disease of domestic pigs characterized rapid progression death within 2 weeks. How immune cells respond to acute ASFV infection contribute immunopathogenesis has not been completely understood. In this study, we examined activation, apoptosis, functional changes distinct in following with CADC_HN09 strain using multicolor flow cytometry. We found that induced broad apoptosis DCs, monocytes, neutrophils,...
Marek's disease virus (MDV) is a highly oncogenic alphaherpesvirus that causes deadly T-cell lymphomas and serves as natural virus-induced tumor model in chickens. The most efficacious vaccine, CVI988/Rispens (CVI988), against MD has been used for several decades. However, the mechanisms leading to protective immunity following vaccination are not fully understood. In this study, employing multi-parameter flow cytometry, we performed comprehensive analysis of T cell responses...
The emerging H5 clade 2.3.4.6 viruses of different NA subtypes have been detected in domestic poultry China. We evaluated the receptor binding property and transmissibility four novel subtype highly pathogenic avian influenza viruses. results show that these bound to both avian-type (α-2,3) human-type (α-2,6) receptors. Furthermore, we found one viruses, GS/EC/1112/11, not only replicated but also transmitted efficiently guinea pigs. Therefore, such potential a pandemic threat.
Avian influenza virus (AIV) emerged and has continued to re-emerge, continuously posing great threats animal human health. The detection of hemagglutination inhibition (HI) or neutralization antibodies (NA) is essential for assessing immune protection against AIV. However, the HI/NA-independent constantly observed in vaccines’ development H7N9 subtype AIV other subtypes chickens mammals, necessitating analysis cellular response. Here, we established a multi-parameter flow cytometry examine...
Pseudomonas aeruginosa is a ubiquitous environmental bacterium that causes variety of infections in humans and animals. Although antibiotic resistance livestock has been extensively documented, continuous surveillance remains crucial for tracking emerging trends assessing control measures. During 2017 2018, 234 strains P. were identified from 1063 pathogenic nonpathogenic bacteria isolated raw milk healthy mastitis cows. In this study, 132 convenience isolates recovered tested antimicrobial...
<title>Abstract</title> Infection with genotype VII Newcastle disease virus (NDV) poses a significant threat to poultry health, characterized by severe damage immune organs such as lymphocyte depletion. However, the precise mechanisms underlying this phenomenon remain unclear. Our present investigation focused on dynamic changes of macrophages, T cells, and B cells in spleen chickens infected NDV using multicolor flow cytometry. We found that selectively chicken splenic macrophages...
Wolbachia are maternally transmitted intracellular bacteria that can naturally and artificially infect arthropods nematodes. Recently, they were applied to control the spread of mosquito-borne pathogens by causing cytoplasmic incompatibility (CI) between germ cells females males. The ability induce CI is based on prevalence polymorphism in natural populations mosquitoes. In this study, we screened infection level diversity field-collected mosquitoes from 25 provinces China partial sequence...
The molecular bases of adaptation and pathogenicity H9N2 influenza virus in mammals are largely unknown. Here, we show that a mouse-adapted PB2 gene with phenylalanine-to-leucine mutation (F404L) mainly contributes to enhanced polymerase activity, replication, mice also increases the virulence H5N1 2009 pandemic H1N1 viruses. Therefore, defined novel pathogenic determinant, providing further insights into pathogenesis viruses mammals.
H9N2 avian influenza virus (AIV) of genotype S frequently donate internal genes to facilitate the generation novel reassortants such as H7N9, H10N8, H5N2 and H5N6 AIVs, posing an enormous threat both human health poultry industry. However, pathogenicity transmission reassortant H5 viruses with gene cassette H9N2-origin in chickens mice remain unknown. In this study, four carrying HA NA from different clades remaining predominant were generated by reverse genetics. We found that all showed...
The CD69 molecule, as an early activation marker of lymphocytes, is often used to assess the cellular immunity. However, for pigs, anti-pig antibody not yet available this purpose after infection or vaccination. In study, a monoclonal (mAb) against pig was produced by peptide immunization and hybridoma technique. One mAb (5F12) showed good reactivity with that expressed in transfected-HEK-293T cells on mitogen-activated porcine peripheral blood mononuclear (PBMCs) indirect immunofluorescence...
Bovine leukemia virus (BLV) causes enzootic bovine leucosis and is widely spread worldwide, except several European countries, Australia New Zealand. Although BLV highly prevalent in China, information about the genetic diversity evolutionary dynamics of among Chinese dairy herds still lacking. To determine variability BLV, 219 cows from four cities Ningxia province China were screened for infection by fluorescence resonance energy transfer (FRET)-PCR sequencing, 16 selected positive samples...
Genotype S H9N2 avian influenza virus, which has been predominant in China since 2010, contributed its entire internal gene cassette to the genesis of novel reassortant viruses, including H5Nx, H7N9 and H10N8 viruses that pose great threat poultry humans. A key feature genotype virus is substitution G1-like M PB2 genes for earlier F/98-like H virus. However, how this influenced viral adaptability emerging mammals remains unclear. We report here H5Nx with displayed enhanced replication...
Highly pathogenic (HP) H7N9 avian influenza virus (AIV) emerged in China 2016. HP AIV caused at least 33 human infections and has been circulating poultry farms continuously since wave 5. The genetic divergence, geographic patterns, hemagglutinin adaptive parallel molecular evolution of 2017 are still unclear. Here, 10 new strains AIVs from October 2019 to April 2021 were sequenced. We found that was primarily Northern China, particularly the provinces surrounding Bohai Sea (Liaoning, Hebei,...
Bovine leukemia virus (BLV) is widespread in global cattle populations, but the effects of its infection on milk quantity and quality have not been clearly elucidated animal models. In this study, 30 healthy first-lactation cows were selected from ≈2,988 a BLV-free farm with same criteria parity, age, lactation number, as well yield, SCS, composition (fat, protein, lactose). Subsequently, these randomly assigned to intervention (n = 15) or control group, reared different cowsheds. Cows group...
Reassortment with the H9N2 virus gave rise to zoonotic H7N9 avian influenza (AIV), which caused more than five outbreak waves in humans, high mortality. The frequent exchange of genomic segments between and has been well-documented. However, reassortment patterns have not described are yet fully understood. Here, we used phylogenetic analyses investigate intersubtype intrasubtype/intralineage across eight viral segments. its progeny frequently exchanged internal genes but rarely other AIV...
The use of antibiotics is the preferred therapy for bacterial diseases. However, overusing has led to development antibiotic resistance in bacteria, which now a major public health concern. Therefore, this study, performance lysozyme (LYZ)/tracheal antimicrobial peptide (TAP)-based tissue-specific expression plasmids (TSEAP) have been evaluated treatment mastitis mice. results show that LYZ/ and TAP-based TSEAP could effectively reduce clinical symptoms caused by Staphylococcus sciuri,...