A5052136942- Insect symbiosis and bacterial influences
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Invertebrate Immune Response Mechanisms
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Tuberculosis Research and Epidemiology
- Genomics and Phylogenetic Studies
- Mosquito-borne diseases and control
- Historical Medical Research and Treatments
- Bacterial biofilms and quorum sensing
- Insect-Plant Interactions and Control
- Plant and Fungal Interactions Research
- Monoclonal and Polyclonal Antibodies Research
- Antibiotic Resistance in Bacteria
- Plant Virus Research Studies
- Evolution and Genetic Dynamics
- Pneumocystis jirovecii pneumonia detection and treatment
- Malaria Research and Control
- Antifungal resistance and susceptibility
- Plant Disease Management Techniques
- Hepatitis B Virus Studies
The Pirbright Institute
2016-2024
Inserm
2024
Hebrew University of Jerusalem
2024
Imperial College London
2024
Forest Research
2023-2024
The University of Texas at Arlington
2009
University of Notre Dame
2002-2005
Gdańsk Medical University
1999-2005
The molecular pathways controlling gender are highly variable and have been identified in only a few nonmammalian model species. In many insects, maleness is conferred by Y chromosome-linked M factor of unknown nature. We isolated characterized gene, Yob, for the malaria mosquito Anopheles gambiae activated at beginning zygotic transcription expressed throughout male's life, controls male-specific splicing doublesex gene. Silencing embryonic Yob expression male-lethal, whereas ectopic...
Abstract Engineered sex ratio distorters (SRDs) have been proposed as a powerful component of genetic control strategies designed to suppress harmful insect pests. Two types CRISPR-based SRD mechanisms proposed: X-shredding, which eliminates X-bearing sperm, and X-poisoning, females inheriting disrupted X-chromosomes. These differences can profound impact on the population dynamics SRDs when linked Y-chromosome: an X-shredder is invasive, constituting classical meiotic Y-drive, whereas...
The insect sex determination and the intimately linked dosage compensation pathways represent a challenging evolutionary puzzle that has been solved only in Drosophila melanogaster. Analyses of orthologs genes identified non-drosophilid taxa1,2 revealed evolution is consistent with bottom-up mode,3 where terminal within pathway are well conserved. doublesex (dsx), occupying bottom-most position encoding sex-specific proteins orchestrating downstream sexual differentiation processes, an...
Dosage compensation is the fundamental process by which gene expression from male monosomic X chromosome and diploid set of autosomes equalized. Various molecular mechanisms have evolved in different organisms to achieve this task. In Drosophila , genes on are upregulated levels two chromosomes females. To test whether a similar mechanism operating immature stages Anopheles mosquitoes, we analyzed global gambiae fourth instar larvae pupae using high-coverage RNA-seq data. both sexes, median...
Acquisition of genetic information through horizontal gene transfer (HGT) is an important evolutionary process by which micro-organisms gain novel phenotypic characteristics. In pathogenic bacteria, for example, it facilitates maintenance and enhancement virulence spread drug resistance. the genus Mycobacterium , to several primary human pathogens belong, HGT has not been clearly demonstrated. The few existing reports suggesting this are based on circumstantial evidence similarity sequences...
Dosage compensation (DC), a process countering chromosomal imbalance in individuals with heteromorphic sex chromosomes, has been molecularly characterized only mammals, Caenorhabditis elegans, and fruit flies.1 In Drosophila melanogaster males, it is achieved by an approximately 2-fold hypertranscription of the monosomic X chromosome mediated MSL complex.2,3 The complex not assembled on female chromosomes because production its key protein MSL-2 prevented due to intron retention inhibition...
Summary Mycobacterium avium is a major opportunistic pathogen of AIDS patients in the United States. The understanding M. pathogenesis has been hampered by inability to create gene knockouts homologous recombination, an important mechanism for defining and characterizing virulence factors. In this study functional methyltransferase D ( mtfD ) was deleted allelic replacement strain 104. Methyltransferase involved methylation glycopeptidolipids (GPLs); highly antigenic glycolipids found...
Mosquito-borne diseases, such as malaria, are controlled primarily by suppressing mosquito vector populations using insecticides. The current control programmes seriously threatened the emergence and rapid spread of resistance to approved Genetic approaches proposed complement existing efforts may be a more sustainable solution control. All would rely on releases modified male mosquitoes, because released females contribute biting pathogen transmission. However, no sufficiently large-scale...
Male mosquitoes do not feed on blood and are involved in delivery of pathogens to humans. Consequently, they seldom the subjects research, which results a very poor understanding their biology. To gain insights into male developmental processes we sought identify genes transcribed exclusively reproductive tissues Anopheles gambiae pupae. Using cDNA subtraction strategy, five male-specifically or highly male-biased expressed were isolated, four remain unannotated An. genome. Spatial temporal...
<title>Abstract</title> Engineered sex ratio distorters (SRDs) have been proposed as a powerful component of genetic control strategies designed to suppress harmful insect pests. Two types CRISPR-based SRD mechanisms proposed: X-shredding eliminates X-bearing sperm, while X-poisoning daughters inheriting disrupted X-chromosomes. These differences can profound impact on the population dynamics SRDs when linked Y-chromosome: an X-shredder is invasive constituting classical meiotic Y-drive,...
The Mycobacterium avium complex (MAC) encompasses two species, M. and intracellulare , which are opportunistic pathogens of humans animals. standard method MAC strain differentiation is serotyping based on a variation in the antigenic glycopeptidolipid (GPL) composition. To elucidate relationships among serotypes phylogenetic analysis 13 reference clinical strains from 8 was performed using as markers genomic regions (890 bp gtfB gene 2150 spanning rtfA – mtfC genes) associated with strains'...
Abstract Engineered sex ratio distorters (SRDs) have been proposed as a powerful component of genetic control strategies designed to suppress harmful insect pests. Two types CRISPR-based SRD mechanisms proposed: X-shredding eliminates X-bearing sperm, while X-poisoning daughters inheriting disrupted X-chromosomes. These differences can profound impact on the population dynamics SRDs when linked Y-chromosome: an X-shredder is invasive constituting classical meiotic Y-drive, whereas...
In England, Cryphonectria parasitica was detected for the first time in 2011 a nursery and 2016 wider environment. Surveys between 2017 2020 identified disease at different sites Berkshire, Buckinghamshire, Cornwall, Derbyshire, Devon, Dorset, London, West Sussex, island of Jersey, while present study comprises results 2020-2023 survey with findings Kent, Nottinghamshire, Herefordshire, Leicestershire, islands Jersey Guernsey. A total 226 suspected samples were collected from 72 surveyed...
Cell lines allow studying various biological processes that may not be easily tractable in whole organisms. Here, we have established the first male-specific cell line from African malaria mosquito, Anopheles gambiae. The cells, named AgMM and derived sex-sorted neonate larvae, were able to undergo spontaneous contractions for a number of passages following establishment, indicating their myoblast origin. Comparison transcriptome an A. gambiae-derived Sua5.1 hemocyte cells revealed...
Abstract Cell lines allow studying various biological processes that may not be easily tractable in whole organisms. Here, we have established the first male-specific cell line from African malaria mosquito, Anopheles gambiae . The cells, named AgMM and derived sex-sorted neonate larvae, were able to undergo spontaneous contractions for a number of passages following establishment, indicating their myoblast origin. Comparison transcriptome an A. -derived Sua5.1 hemocyte cells revealed...