The responses of 14 potato cultivars to five Potato virus Y (PVY) isolates belonging four strains (ordinary [PVYO], tobacco veinal necrosis [PVYN], N:O group [PVYN:O], and nonrecombinant tuber necrotic [PVYNTN]) were studied in primary secondary infections. For the infection experiments, foliage symptoms monitored daily after mechanical inoculation with a PVY isolate until harvest; and, for regularly from plant emergence harvest. Tuber (namely, ringspots) checked at harvest monthly...

(1996). Nucleotide sequence and genome organization of a Canadian isolate the common strain potato virus Y (PVY°) Journal Plant Pathology: Vol. 18, No. 3, pp. 209-224.

Previous studies have identified a recombinant isolate group of the Potato virus Y (PVY), PVYN:O, which exhibits PVYO serotype and PVYN pathotype. Molecular fingerprinting indicated that isolates possess European (Eu)–PVYN/NTN-like P1 gene one PVYN-PVYO joint at HC/Pro-P3 site. In this study, complete genomic RNA sequence two PVYN:O was determined. The overall identities polyprotein level between other ranged from 94.1% to 97.2%. However, varied 70.9% 100% when individual mature proteins...

Abstract BACKGROUND: The objectives of this work were to determine if the control potato virus Y (PVY, genus Potyvirus , family Potyviridae ) in seed could be improved by combining border crops and mineral oil sprays, crop acts as a barrier or sink. RESULTS: Field tests over 3 years confirmed that oils alone are an effective PVY, showed borders act PVY Combining familiar more recent methods was almost twice reducing incidence either one used alone. combination provided consistently high...

Over 40 isolates of potato spindle tuber viroid (PSTVd) have been reported from potato, other Solanum species and greenhouse tomato. These sequence similarities in the range 95-99%. A which caused chlorotic leaves severe dwarfing plants tomato crops was detected. The found to hybridize readily with PSTVd probes. It migrated faster than return-polyacrylamide gel electrophoresis not amplified RT-PCR by a primer pair based on lower strand central conserved region PSTVd. Nucleotide sequencing...

Surveys of commercial and seed potato fields for virus diseases (1998 to 2002) in Manitoba established that Potato Y (PVY) is concern production. To determine the prevalence PVY strains, PVY-infected tubers identified by reverse transcription-polymerase chain reaction (RT-PCR) from surveys (2000 2001) were grown symptom expression strain characterization strain-specific RT-PCR, bioassays, serological assays. Of samples collected tested 4.0% contained PVY. Further analysis PVY-positive a...

Potato virus Y (PVY) exists as several strains with distinct symptomology and tuber yield effects in different potato varieties. Recently, new recombinant have proliferated dominated local populations around the world. In this study, PVYO, PVYN:O, PVYN-Wi, PVYNTN were tracked across Canada from 2014 to 2017, showing rapid evolution of away traditionally dominant PVYO recombinants PVYN-Wi (western Canada) (eastern Canada). Simultaneously, 30 varieties inoculated controlled greenhouse...

The current-season spread of Potato virus Y (PVY) was monitored in 19 fields under various management practices New Brunswick, Canada, through the 2011 and 2012 growing seasons. focus this study to evaluate role seedborne PVY inoculum, aphid vector abundance, numbers, timing, types insecticide mineral oil sprays, confirm reliability forecasting capacity midseason testing. In each field, 100 110 virus-free plants were identified shortly after emergence assessed four times from early July...

A field isolate of Potato virus Y (PVY) was collected in New Brunswick, Canada 2007 due to unusual symptoms observed on different potato cultivars. To unveil the PVY strain identity, tobacco and bioassays, PVYO PVYN-specific antibody-based enzyme-linked immunosorbent assays, reverse-transcription polymerase chain reaction (PCR)-based genotyping were carried out. All assays demonstrated that isolate, designated as PVYO-FL this study, belonged group. Greenhouse tests with cvs. FL 1533 Jemseg...

The current-season spread of Potato virus Y (PVY) was investigated in New Brunswick, Canada, 11 potato fields planted with six different cultivars 2009 and 2010. In all, 100 plants selected from each field were monitored for PVY infections using enzyme-linked immunosorbent assay (ELISA) real-time reverse-transcription polymerase chain reaction (RT-PCR) assay. Average incidence increased 0.6% 2% 2010 the leaves to 20.3% 21.9% tubers at time harvest. individual fields, reached as high 37% 39%...

Sequence analysis of the chromosome region harboring sequence-tagged site (STS) markers YES3-3A and YES3-3B for Ry sto , a gene responsible extreme resistance to Potato virus Y (PVY) in potato, was performed tetraploid potato ‘Barbara’ (Rrrr) ‘AC Chaleur’ (rrrr) as well their progeny selections. Three two sequence variants were identified Barbara resistant (R) selections AC Chaleur susceptible (S) selections, respectively. Further indicates that variant with 21-nucleotide (nt) deletion is...

Aphids are viral vectors in potatoes, most importantly of Potato virus Y (PVY), and insecticides frequently used to reduce spread during the crop season. collected from potato belt New Brunswick, Canada, 2015 2016 were surveyed for known novel mutations Na-channel (para) gene, coding target synthetic pyrethroid insecticides. Specific genetic confer resistance (kdr skdr) found great abundance Myzus persicae (Sulzer) (Hemiptera: Aphididae), which rose 76% 96% 2016. other than M. showed lower...