A5080522760- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Circadian rhythm and melatonin
- RNA Research and Splicing
- Evolution and Genetic Dynamics
- Neuroendocrine regulation and behavior
- Renal and related cancers
- Spaceflight effects on biology
- RNA modifications and cancer
- Light effects on plants
- RNA and protein synthesis mechanisms
- Cell Image Analysis Techniques
- Diet and metabolism studies
- Neurobiology and Insect Physiology Research
- Immunotherapy and Immune Responses
- Rabbits: Nutrition, Reproduction, Health
- Social and Educational Sciences
- Research in Social Sciences
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Identity, Memory, and Therapy
- Advanced biosensing and bioanalysis techniques
- Anesthesia and Neurotoxicity Research
- Agriculture Sustainability and Environmental Impact
Howard Hughes Medical Institute
2022-2024
Columbia University
2022-2024
Tel Aviv University
2015-2022
It is unknown whether the activity of nervous system can be inherited. In Caenorhabditis elegans nematodes, parental responses transmit heritable small RNAs that regulate gene expression transgenerationally. this study, we show a neuronal process impact next generations. Neurons-specific synthesis RDE-4-dependent regulates germline amplified endogenous interfering (siRNAs) and for multiple Further, production in neurons controls chemotaxis behavior progeny at least three generations via...
Homeobox genes are prominent regulators of neuronal identity, but the extent to which their function has been probed in animal nervous systems remains limited. In nematode Caenorhabditis elegans, each individual neuron class is defined by expression unique combinations homeobox genes, prompting question whether indeed requires a gene for its proper identity specification. We present here progress addressing this extending previous mutant analysis family members and describing multiple...
Despite progress in systemic small interfering RNA (siRNA) delivery to the liver and solid tumors, siRNA leukocytes remains challenging. The ability silence gene expression has great potential for identifying drug targets RNAi-based therapy leukocyte diseases. However, both normal malignant are among most difficult as they resistant conventional transfection reagents dispersed body. We used mantle cell lymphoma (MCL) a prototypic blood cancer validating novel strategy. MCL is an aggressive...
It is unknown whether transient transgenerational epigenetic responses to environmental challenges affect the process of evolution, which typically unfolds over many generations. Here, we show that in C. elegans, inherited small RNAs control genetic variation by regulating crucial decision self-fertilize or outcross. We found under stressful temperatures, younger hermaphrodites secrete a male-attracting pheromone. Attractiveness transmits transgenerationally unstressed progeny via heritable...
Mapping neurotransmitter identities to neurons is key understanding information flow in a nervous system. It also provides valuable entry points for studying the development and plasticity of neuronal identity features. In Caenorhabditis elegans system, have been largely assigned by expression pattern analysis pathway genes that encode biosynthetic enzymes or transporters. However, many these assignments relied on multicopy reporter transgenes may lack relevant cis -regulatory therefore not...
Mapping neurotransmitter identities to neurons is key understanding information flow in a nervous system. It also provides valuable entry points for studying the development and plasticity of neuronal identity features. In Caenorhabditis elegans system, have been largely assigned by expression pattern analysis pathway genes that encode biosynthetic enzymes or transporters. However, many these assignments relied on multicopy reporter transgenes may lack relevant cis -regulatory therefore not...
Caenorhabditis elegans nematodes are powerful model organisms, yet quantification of visible phenotypes is still often labor-intensive, biased, and error-prone. We developed WorMachine, a three-step MATLAB-based image analysis software that allows (1) automated identification C. worms, (2) extraction morphological features fluorescent signals, (3) machine learning techniques for high-level analysis.
One avenue to better understand brain evolution is map molecular patterns of evolutionary changes in neuronal cell types across entire nervous systems distantly related species. Generating whole-animal single-cell transcriptomes three nematode species from the Caenorhabditis genus, we observed a remarkable stability neuronal-cell-type identities over more than 45 million years evolution. Conserved combinatorial expression homeodomain transcription factors are among best classifiers...
Assigning neurotransmitter identity to neurons is key understanding information flow in a nervous system. It also provides valuable entry points for studying the development and plasticity of neuronal features. Neurotransmitter identities have been assigned most C. elegans system through expression pattern analysis pathway genes that encode biosynthetic enzymes or transporters. However, many these assignments relied on multicopy reporter transgenes may lack relevant cis -regulatory therefore...
One avenue to better understand brain evolution is map molecular patterns of evolutionary changes in neuronal cell types across entire nervous systems distantly related species. Generating whole-animal single-cell transcriptomes three nematode species from the
Mapping neurotransmitter identities to neurons is key understanding information flow in a nervous system. It also provides valuable entry points for studying the development and plasticity of neuronal identity features. In C. elegans system, have been largely assigned by expression pattern analysis pathway genes that encode biosynthetic enzymes or transporters. However, many these assignments relied on multicopy reporter transgenes may lack relevant cis -regulatory therefore not provide an...
Mapping neurotransmitter identities to neurons is key understanding information flow in a nervous system. It also provides valuable entry points for studying the development and plasticity of neuronal identity features. In C. elegans system, have been largely assigned by expression pattern analysis pathway genes that encode biosynthetic enzymes or transporters. However, many these assignments relied on multicopy reporter transgenes may lack relevant cis-regulatory therefore not provide an...
Abstract While Caenorhabditis elegans nematodes are powerful model organisms, quantification of visible phenotypes is still often labor-intensive, biased, and error-prone. We developed “WorMachine”, a three-step MATLAB-based image analysis software that allows automated identification C. worms, extraction morphological features, fluorescent signals. The program offers machine learning techniques which should aid in studying large variety research questions. demonstrate the power WorMachine...
Abstract In C. elegans nematodes, components of liquid-like germ granules were shown to be required for transgenerational small RNA inheritance. Surprisingly, we show here that mutants with defective ( pptr-1 , meg-3/4 pgl-1 ) can nevertheless inherit potent RNA-based silencing responses, but some the lose this ability after many generations homozygosity. Animals mutated in which is stabilization P early embryo, display extremely strong heritable RNAi last tens generations, long responses...
Abstract It is unknown whether transient transgenerational epigenetic responses to environmental challenges affect the process of evolution, which typically unfolds over many generations. Here we show that in C. elegans , inherited small RNAs shape hard-wired genome and control genetic variation by regulating decision self-fertilize or outcross. We found under stressful temperatures younger hermaphrodites secrete a male-attracting pheromone. Attractiveness transmits transgenerationally...
ABSTRACT Homeobox genes are prominent regulators of neuronal identity, but the extent to which their function has been probed in animal nervous systems remains limited. In nematode Caenorhabditis elegans , each individual neuron class is defined by expression unique combinations homeobox genes, prompting question whether indeed requires a gene for its proper identity specification. We present here progress addressing this extending previous mutant analysis family members and describing...
The maturation of genome editing techniques dramatically broadens the range organisms amenable to mechanistic investigation.