A5014415722- CRISPR and Genetic Engineering
- Machine Learning in Bioinformatics
- Pluripotent Stem Cells Research
- Chemical Synthesis and Analysis
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- vaccines and immunoinformatics approaches
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Cancer-related gene regulation
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Reproductive Biology and Fertility
Duke University
2022-2023
Massachusetts Institute of Technology
2022-2023
Human Media
2022-2023
MIT-Harvard Center for Ultracold Atoms
2022
Abstract CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking guide RNA-programmed target site, limiting their sequence accessibility for robust genome editing applications. In this study, we recombine the PAM-interacting domain of SpRY, broad-targeting Cas9 possessing an NRN > NYN (R = A or G, Y C T) PAM preference, with N-terminus Sc + +, simultaneously broad, efficient, and accurate NNG capabilities, to generate chimeric enzyme highly flexible preference: SpRYc....
A bstract Therapeutic modalities targeting pathogenic proteins are the gold standard of treatment for multiple disease indications. Unfortunately, a significant portion these considered “undruggable” by small molecule-based approaches, largely due to their disordered nature and instability. Designing functional peptides undruggable targets, either as standalone binders or fusions effector domains, thus presents unique opportunity therapeutic intervention. In this work, we adapt recent models...
CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking guide RNA-programmed target site, limiting their sequence accessibility for robust genome editing applications. In this study, we recombine the PAM-interacting domain of SpRY, broad-targeting Cas9 possessing an NRN > NYN PAM preference, with N-terminus Sc++, simultaneously broad, efficient, and accurate NNG capabilities, to generate chimeric enzyme highly flexible preference: SpRYc. We demonstrate that SpRYc leverages...
Abstract Germ cells are the vehicle of human reproduction, arising early in embryonic development and developing throughout adult life until menopause onset women. Primordial germ common precursors germline both sexes, undergoing sexual specification into oogonia or gonocytes which further develop oocytes spermatocytes during development. Methods for recapitulation primordial cell formation have been developed extensively recent decades, but fundamental technical limitations their...
Abstract Here, we integrate fine-tuned protein language models and protein-protein interaction databases to develop a Structure-agnostic Language Transformer & Peptide Prioritization module that efficiently selects peptides from interfaces, without the need for structural information. We experimentally fuse SaLT&PepPr-derived “guide” E3 ubiquitin ligase domains reliably identify candidates induce robust intracellular degradation of clinically-relevant targets, exhibit high binding...