A5113160125- Spine and Intervertebral Disc Pathology
- Spinal Fractures and Fixation Techniques
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Nerve injury and regeneration
- Spinal Cord Injury Research
- Cervical and Thoracic Myelopathy
- Graphene and Nanomaterials Applications
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Silk-based biomaterials and applications
- Diabetes and associated disorders
- Reproductive System and Pregnancy
- 3D Printing in Biomedical Research
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Bone Metabolism and Diseases
- Crystallization and Solubility Studies
- Diabetes Management and Research
- RNA modifications and cancer
- Pharmacological Effects of Natural Compounds
- Tissue Engineering and Regenerative Medicine
- Traditional Chinese Medicine Studies
- Plant-based Medicinal Research
- Inflammatory Bowel Disease
Soochow University
2016-2025
Nanjing Medical University
2023-2025
First Affiliated Hospital of Soochow University
2016-2025
Nanjing University of Chinese Medicine
2022-2025
Jiangsu Province Hospital
2023-2025
Central South University
2024
Hainan Provincial Hospital of Traditional Chinese Medicine
2019-2024
Macao Polytechnic University
2024
CM Hospital
2023-2024
Zhejiang Chinese Medical University
2022-2024
Caspases are essential components of the mammalian cell death machinery. Here we test hypothesis that Caspase 9 (Casp9) is a critical upstream activator caspases through gene targeting in mice. The majority Casp9 knockout mice die perinatally with markedly enlarged and malformed cerebrum caused by reduced apoptosis during brain development. deletion prevents activation Casp3 embryonic brains vivo, Casp9-deficient thymocytes show resistance to subset apoptotic stimuli, including absence...
The interleukin-1β (IL-1β) converting enzyme (ICE) processes the inactive IL-1β precursor to proinflammatory cytokine. ICE was also shown cleave of interferon-γ inducing factor (IGIF) at authentic processing site with high efficiency, thereby activating IGIF and facilitating its export. Lipopolysaccharide-activated ICE-deficient (ICE −/− ) Kupffer cells synthesized but failed process it into active form. Interferon-γ were diminished in sera mice exposed Propionibacterium acnes...
Abstract Fully simulating the components and microstructures of soft tissue is a challenge for its functional regeneration. A new aligned hydrogel microfiber scaffold spinal cord regeneration constructed with photocrosslinked gelatin methacryloyl (GelMA) electrospinning technology. The directional porous fibrous consistent nerve axons vital to guide cell migration axon extension. GelMA electrospun fibers soak up water more than six times their weight, lower Young's modulus, providing...
The strategies concerning modification of the complex immune pathological inflammatory environment during acute spinal cord injury remain oversimplified and superficial. Inspired by acidic microenvironment at sites, a functional pH-responsive immunoregulation-assisted neural regeneration strategy was constructed. With capability directly responding to focal areas followed triggered release IL-4 plasmid-loaded liposomes within few hours suppress cytokines promote differentiation mesenchymal...
Although injectable hydrogel microsphere has demonstrated tremendous promise in clinical applications, local overactive inflammation degenerative diseases could jeopardize biomaterial implantation's therapeutic efficacy. Herein, an "peptide-cell-hydrogel" was constructed by covalently coupling of APETx2 and further loading nucleus pulposus cells, which inhibit inflammatory cytokine storms to regulate the metabolic balance ECM vitro. The covalent preserved biocompatibility microspheres...
We have analyzed the cell cycle regulation of human cyclin-dependent kinase 2 (CDK2), a protein closely related to cycle-regulatory CDC2. find that CDK2 activity, like CDC2, oscillates during in cultured mammalian fibroblasts. Unlike CDC2 activity (which peaks mitosis), rises late G1 or early S phase and declines mitosis. Active S-phase migrates multiple large complexes on gel filtration, one these is associated with cyclin A. These findings suggest association distinct cyclins, regulate...
Interleukin-1β-converting enzyme (ICE) is a novel cysteine protease responsible for the cleavage of pre-interleukin-1β (pre-IL-1β) to mature cytokine and member family related proteases (the caspases) that includes Caenorhabditis elegans cell death gene product, CED-3. In addition their sequence homology, these display an unusual substrate specificity peptidyl sequences with P1 aspartate residue. We have examined kinetics processing pre-IL-1β form by ICE three its homologs, TX, CPP-32,...
We have analyzed the activation of human cyclin-dependent kinases in a cell-free system. Human CDC2, kinase 2 (CDK2), cyclin A, and B1 were produced insect cells by infection with recombinant baculoviruses. CDC2 or CDK2 monomers lysates infected could be activated addition containing A B1. B1, as well cyclins was accompanied formation high molecular weight complexes. In contrast, did not bind effectively to A. studied detail found phosphorylation on Threonine 161. The binding also occurred...
We have identified and characterized a novel cysteine protease named CMH-1 that is new member of the interleukin 1 beta converting enzyme (ICE) family proteases with substrate specificity for Asp-X. has highest similarity to CPP32 (52% amino acid identity) MCH2 (31% identical). shares conserved residues form core structure ICE as well those involved in catalysis P1 aspartate binding. Overexpression COS cells resulted processing induction apoptosis transfected cells. Coexpression...
Recently, human interleukin 18 (hIL-18) cDNA was cloned, and the recombinant protein with a tentatively assigned NH2-terminal amino acid sequence generated. However, natural hIL-18 has not yet been isolated, its cellular processing is therefore still unclear. To clarify this, we purified from cytosolic extract of monocytic THP.1 cells. Natural exhibited molecular mass 18.2 kDa, Tyr37. Biological activities were identical to those respect enhancement killer cell cytotoxicity interferon-γ...
Adipose tissue from patients with HIV-related lipodystrophy presents a state of chronic inflammation. Altered expression cytokines/adipokines and macrophage infiltration could be involved in patients' insulin resistance lipoatrophy. We tested whether antiretrovirals affected adipokine release by human subcutaneous adipocytes cytokine/chemokine production macrophages examined reactive oxygen species (ROS) hyperproduction was related to the effect antiretrovirals.Differentiated PMA-THP-1 were...
// Amy B. Hall 1,* , Dave Newsome Yuxin Wang Diane M. Boucher 1 Brenda Eustace Yong Gu Brian Hare Mac A. Johnson Howard Li Sean Milton Cheryl E. Murphy Darin Takemoto Crystal Tolman Mark Wood Peter Charlton 2 Jean-Damien Charrier Brinley Furey Julian Golec Philip Reaper and John R. Pollard Vertex Pharmaceuticals Inc, Boston, MA, USA (Europe) Ltd, Park, Abingdon, Oxfordshire, UK * These authors contributed equally to this work Correspondence: R email: Keywords : ATR, DNA damage response,...
Periosteum plays the pivotal role in neomineralization, vascularization and protection during bone tissue regeneration. However, many artificial periosteum focused only on lacked of osteogenesis angiogenesis functional capacity. In this study, we developed a novelty inorganic strengthened gelatin hydrogel membrane via organic co-cross-linked double network as for enhancing durable reconstruction. Mesoporous bioactive glass nanoparticles (MBGNs) chemically modified with photo-cross-linkable...
Current homogeneous bioscaffolds could hardly recapture the regenerative microenvironment of extracellular matrix. Inspired by peculiar nature dura matter, we developed an matrix-mimicking scaffold with biomimetic heterogeneous features so as to fit multiple needs in mater repairing. The inner surface endowed anisotropic topology and optimized chemical cues orchestrate elongation bipolarization fibroblasts preserve quiescent phenotype indicated down-regulated α-smooth muscle actin...
Recombinant human bone morphogenetic protein-2 (rhBMP-2) and bioceramic are the widely used bioactive factors in treatment of defects, but these easily cause side effects because uncontrollable local concentration. In this study, rhBMP-2 was grafted on surface mesoporous bioglass nanoparticles (MBGNs) with an amide bond then photo-cross-linked together methacrylate gelatin (GelMA); way, a GelMA/MBGNs-rhBMP-2 hydrogel membrane fabricated to release controllable program during early...
Abstract Antagonist therapy represents a potential treatment for extracellular matrix (ECM) metabolic imbalance via the specific binding of inflammatory factors resulting from inflammation. However, short half‐life antagonist bioactivity creates challenges their clinical application. Herein, bovine serum albumin nanoparticles (BNP) encapsulating recombinant human soluble tumor necrosis factor (TNF) receptor type II (rhsTNFRII) are grafted onto microfluidic poly( l ‐lactic acid) (PLLA) porous...
The inflammatory cascade after spinal cord injury (SCI) causes necrotizing apoptosis of local stem cells, which limits nerve regeneration. Therefore, coordinating the immune response and neural cell (NSC) functions is key to promoting recovery central nervous system function. In this study, a hydrogel "perfusion" electrospinning technology are integrated, "concrete" composite support for repair injuries built. hydrogel's hydrophilic properties activate macrophage integrin receptors mediate...
Objectives: To analyze and describe the spatiotemporal trends of Low back pain (LBP) burdens from 1990 to 2019 anticipate following decade’s incidence. Methods: Using data Global Burden Disease (GBD) Study, we described net drifts, local age effects, period cohort effects in incidence forecasted rates cases by sex 2020 2029 using Nordpred R package. Results: LBP remained leading cause musculoskeletal disease burden globally across all socio-demographic index (SDI) regions. China is top...
Targeting macrophages can facilitate the site-specific repair of critical bone defects. Herein, a composite hydrogel, gelatin-Bletilla striata polysaccharide-mesoporous bioactive glass hydrogel (GBMgel), is constructed via self-assembly mesoporous on polysaccharide structures, through Schiff base reaction. GBMgel efficiently capture and drive recruitment seed stem cells vascular budding required for regeneration in early stages injury, observed sustained release inorganic silicon ions...