A5068915286- Bacteriophages and microbial interactions
- Ruminant Nutrition and Digestive Physiology
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Gut microbiota and health
- Aquaculture disease management and microbiota
- Bacterial biofilms and quorum sensing
- Monoclonal and Polyclonal Antibodies Research
- Anaerobic Digestion and Biogas Production
- Erythrocyte Function and Pathophysiology
- Plant and fungal interactions
- Escherichia coli research studies
- Glycosylation and Glycoproteins Research
- Biofuel production and bioconversion
- Biochemical and Structural Characterization
- Food composition and properties
- Phagocytosis and Immune Regulation
- Phytase and its Applications
- Probiotics and Fermented Foods
- Water Treatment and Disinfection
- Gout, Hyperuricemia, Uric Acid
- Microbial Community Ecology and Physiology
- Meat and Animal Product Quality
- Porphyrin Metabolism and Disorders
Massey University
2010-2024
AgResearch
2014-2016
Abstract Ruminant livestock are important sources of human food and global greenhouse gas emissions. Feed degradation methane formation by ruminants rely on metabolic interactions between rumen microbes affect ruminant productivity. Rumen camelid foregut microbial community composition was determined in 742 samples from 32 animal species 35 countries, to estimate if this influenced diet, host species, or geography. Similar bacteria archaea dominated nearly all samples, while protozoal...
Ruminant livestock represent the single largest anthropogenic source of potent greenhouse gas methane, which is generated by methanogenic archaea residing in ruminant digestive tracts. While differences between individual animals same breed amount methane produced have been observed, basis for this variation remains to be elucidated. To explore mechanistic production, we measured yields from 22 sheep, revealed that are a reproducible, quantitative trait. Deep metagenomic and...
As global warming increases the geographical range and frequency of Vibrio parahaemolyticus infections, its formation biofilms providing bacteria greater resistance to stress contributing persistence pathogens, is threatening seafood industry. V. has a number advantages leading biofilm formation. This study reviews recent advances in understanding on biotic abiotic surfaces, discusses research gaps mechanism examines promising control strategies overcome current limitations chemical...
Symbiotic associations are ubiquitous in the microbial world and have a major role shaping evolution of both partners. One most interesting mutualistic relationships exists between protozoa methanogenic archaea fermentative forestomach (rumen) ruminant animals. Methanogens reside within on surface as symbionts, interspecies hydrogen transfer is speculated to be main driver for physical observed two groups. In silico analyses several rumen methanogen genomes previously shown that up 5% genes...
Vibrio parahaemolyticus is a marine oriented pathogen; and biofilm formation enables its survival persistence on seafood processing plant, complicating the hygienic practice. The objectives of this study are to assess ability V. isolated from related environments form biofilms, determine effective sodium hypochlorite concentrations required inactivate planktonic cells, evaluate genetic diversity for strong formation. Among nine isolates, PFR30J09 PFR34B02 isolates were identified as forming...
Vibrio parahaemolyticus biofilms on the seafood processing plant surfaces are a potential source of contamination and subsequent food poisoning. Strains differ in their ability to form biofilm, but little is known about genetic characteristics responsible for biofilm development. In this study, pangenome comparative genome analysis V. strains reveals attributes gene repertoire that contribute robust formation. The study identified 136 accessory genes were exclusively present strong forming...
Secretins are a family of large bacterial outer membrane channels that serve as exit ports for folded proteins, filamentous phage and surface structures. Despite the size their substrates, secretins do not compromise barrier function membrane, implying gating mechanism. The region in primary structure forms putative gate has previously been determined any secretin. To identify residues involved pIV secretin bacteriophage f1, we used random mutagenesis gene followed by positive selection...
Abstract Proteins are the most diverse structures on bacterial surfaces; hence, they candidates for species‐ and strain‐specific interactions of bacteria with host, environment, other microorganisms. Genomics has decoded thousands surface secreted proteins, yet function cannot be predicted because enormous variability a lack experimental data that would allow deduction through homology. Here, we used phage display to identify pair interacting extracellular proteins in probiotic bacterium L...
In silico, secretome proteins can be predicted from completely sequenced genomes using various available algorithms that identify membrane-targeting sequences. For metasecretome (collection of surface, secreted and transmembrane environmental microbial communities) this approach is impractical, considering the open reading frames (ORFs) comprise only 10% to 30% total metagenome, are poorly represented in dataset due overall low coverage metagenomic gene pool, even large-scale projects. By...
Abstract Complex microbial communities typically contain a large number of low abundance species, which collectively, comprise considerable proportion the community. This ‘rare biosphere’ has been speculated to keystone species and act as repository genomic diversity facilitate community adaptation. Many environmental microbes are currently resistant cultivation, can only be accessed via culture‐independent approaches. To enhance our understanding role rare biosphere, we aimed improve their...
Two strains of Gram-negative, anaerobic, rod-shaped bacteria, from an abundant but uncharacterized rumen bacterial group the order 'Christensenellales', were phylogenetically and phenotypically characterized. These strains, designated R-7T WTE2008T, shared 98.6-99.0 % sequence identity between their 16S rRNA gene sequences. WTE2008T clustered together on a distinct branch other Christensenellaceae had <88.1 to closest type-strain Luoshenia tenuis NSJ-44T. The genome sequences 83.6 average...
The formation of biofilms is a survival strategy employed by bacteria to help protect them from changing or unfavourable environments. In this research, 319 genes which govern biofilm in V. parahaemolyticus, as reported 1,625 publications, were analysed using protein-protein-interaction (PPI) network analysis. CsrA was identified motility-sessility switch and regulator. Through robust rank aggregation (RRA) analysis GSE65340, the generation viable but non-culturable (VBNC) cells that may...
Members of the Clostridiales R-7 group are abundant bacterial residents rumen microbiome; however, they poorly characterized. We report complete genome sequences three members group, FE2010, FE2011, and XBB3002, isolated from ruminal contents pasture-grazed dairy cows in New Zealand.
The potential of using commercial peroxyacetic acid (PAA) for Vibrio parahaemolyticus sanitization was evaluated. Commercial PAA 0.005 % (v/v, PAA: 2.24 mg/L, hydrogen peroxide: 11.79 mg/L) resulted in a planktonic cell reduction >7.00 log10 CFU/mL when initial V. cells averaged 7.64 CFU/mL. For on stainless steel coupons, treatment 0.02 8.96 47.16 achieved >5.00 CFU/cm2 reductions biofilm eight strains but not the two strongest formers. 0.05 22.39 117.91 required to inactivate from mussel...
ABSTRACT Xylanibacter ruminicola is an abundant rumen bacterium that produces propionate in a cobalamin (vitamin B 12 )-dependent manner via the succinate pathway. However, extent to which this occurs across ruminal and closely related bacteria, effect of supplementation on expression pathway genes enzymes has yet be investigated. To assess this, we screened 14 strains found almost all produced when supplemented with cobalamin. X. KHP1 was selected for further study, including complete...