Fourier transform infrared (FTIR) and Raman (FT-Raman) methods were used for rapid characterization classification of selected irradiated starch samples. Biochemical changes due to irradiation detected using the two vibrational spectroscopic techniques, canonical variate analysis (CVA) was applied spectral data discriminating samples based on extent irradiation. The O-H (3000-3600 cm(-1)) stretch, C-H (2800-3000 skeletal mode vibration glycosidic linkage (900-950 in both spectra, band water...

Magnetic nanoparticles (Fe3O4) were synthesized by thermal co-precipitation of ferric and ferrous chlorides. The sizes structure the particles characterized using transmission electron microscopy (TEM). size was in range between 9.7 56.4 nm. Cholesterol oxidase (CHO) successfully bound to via carbodiimide activation. FTIR spectroscopy used confirm binding CHO particles. efficiency 98 100% irrespective amount used. Kinetic studies free revealed that stability activity enzyme significantly...

Gold nanorods (GNRs) with different aspect ratios were fabricated through seed-mediated growth and surface activation by alkanethiols for the attachment of antibodies to yield gold nanorod molecular probes (GNrMPs). Multiplex sensing was demonstrated distinct response plasmon spectra GNrMPs binding events three targets (goat anti-human IgG1 Fab, rabbit anti-mouse anti-sheep IgG (H+L)). Plasmonic sensors are highly specific sensitive can be used monitor refractive index changes caused...

ABSTRACT: Infrared (IR) heating provides significant advantages over conventional heating, including reduced time, uniform quality losses, absence of solute migration in food material, versatile, simple, and compact equipment, energy saving. can be applied to various processing operations, namely, drying, baking, roasting, blanching, pasteurization, sterilization. Combinations IR with microwave other common conductive convective modes have been gaining momentum because increased throughput....

Proteins have proven to be particularly attractive as effective ligands in the synthesis of nano- and subnanoscaled materials because their multiple chelating functional groups imparting unique functionalities. However, protein-directed fluorescent metal cluster is still a challenge but promising area research. Here, we report on new water-soluble, stable, Ag clusters via facile, green method using denatured bovine serum albumin (dBSA) stabilizing agent. The dBSA with its 35 free cysteine...

DNA hypermethylation at the promoter of tumour-suppressor genes is tightly correlated with their transcriptional repression and recognized as hallmark majority cancers. Epigenetic silencing tumour suppressor impairs cellular functions activates a cascade events driving cell transformation cancer progression. Here, we examine site-specific spatiotemporal alteration in methylation target region BRCA1 gene promoter, model gene. We have developed programmable CRISPR-Cas9 based demethylase tool...

Recent advances in fluorescent metal nanoclusters have spurred tremendous interest nanomedicine due to the ease of fabrication, excellent biocompatibility, and, more importantly, wavelength-dependent tunability. Herein, we report our findings on BSA-protected gold (AuNCs), ∼2 nm size conjugated with Herceptin (AuNCs-Her), for specific targeting and nuclear localization ErbB2 over-expressing breast cancer cells tumor tissue as a novel agent simultaneous imaging therapy. More interestingly,...

Significance Plasmonic and metamaterial devices require high-performance material building blocks, both plasmonic dielectric, to be useful in any real-world application. Here, we develop dielectric materials that can grown epitaxially into ultrathin ultrasmooth layers with sharp interfaces. We show a superlattice consisting of titanium nitride as component behaves an optical hyperbolic exhibits extremely high photonic density states.

Abstract Wound healing is an obvious clinical concern that can be hindered by inadequate angiogenesis, inflammation, and chronic hypoxia. While exosomes derived from adipose tissue-derived stem cells have shown promise in accelerating carrying therapeutic growth factors microRNAs, intracellular cargo delivery compromised hypoxic tissues due to activated hypoxia-induced endocytic recycling. To address this challenge, we developed a strategy coat oxygen nanobubbles with incorporate them into...

Hypoxia, excessive reactive oxygen species (ROS), and impaired angiogenesis are prominent obstacles to wound healing following trauma surgical procedures, often leading the development of keloids hypertrophic scars. To address these challenges, a novel approach has been proposed, involving cascade enzymatic reaction-based nanocarriers-laden dressing. This advanced technology incorporates superoxide dismutase modified nanobubbles catalase within an alginate hydrogel matrix. The nano chamber...

Multiple pathogenic detection: A simple, sensitive, and inexpensive multiplex pathogen detection scheme, based on novel gold nanorod bioprobes, is developed to detect <102 cfu mL−1 of pathogens. This method sets the stage for first demonstration LSPR sensors using nanorods with different aspect ratios multiple pathogens, specifically sensitively.

Gold and pearls: Multifunctional nanoparticles, each composed of a single, amine-modified gold nanorod, decorated with multiple "pearls" Fe(3)O(4) nanoparticles capped carboxy groups, are prepared. Their effectiveness in simultaneous targeting, dual-mode imaging, photothermal ablation breast cancer cells is demonstrated.

Abstract The adulteration of extra virgin olive oil with cheaper oils is a major problem in the market. In this study, near‐infrared, mid‐infrared, and Raman spectroscopic techniques were used to quantify amount pomace oil. concentration was range between 0 100% 5% increments by weight. Of methods studied, Fourier transform‐Raman spectroscopy gave highest correlation coefficient 0.997 standard error prediction 1.72%. have potential become tool for rapid determination oil, because they are...

ABSTRACT: This study investigated the efficacy of pulsed UV‐light for continuous‐flow milk treatment inactivation Staphylococcus aureus , a pathogenic microorganism frequently associated with safety concerns. Pulsed UV light is an emerging technology, which can be used this pathogen in relatively short time. damages DNA bacteria by forming thymine dimers that lead to bacterial death. The effect sample distance from quartz window source, number passes, and flow rate was investigated. A...

In this study, we report the chemical synthesis and functionalization of magnetic gold-coated nanoparticles immobilization single-stranded biotinylated oligonucleotides onto these particles. Selected sequences specific to BRCA1 gene were used as a test platform. The binding particles was achieved through streptavidin-biotin bridge via carbodiimide activation protocol. Particle size oligonucleotide attachment confirmed by transmission electron microscopy; characterized Fourier transform...

Multifunctional nanoparticles possessing magnetization and near-infrared (NIR) absorption have warranted interest due to their significant applications in magnetic resonance imaging, diagnosis, bioseparation, target delivery, NIR photothermal ablation. Herein, the site-selective assembly of onto ends or sides gold nanorods with different aspect ratios (ARs) create multifunctional decorated varying numbers particles is described for first time. The resulting hybrid are designated as...